Fig 2: The expression levels of hub genes in GC. (A) The mRNA expression was correlated with pathological T stages. The asterisk represents the comparison between a specific stage and the normal group, or between different stages. * p < 0.05, ** p < 0.01, *** p < 0.001. (B) Representative western blotting for COL10A1, BGN, and FAP proteins in 4 paired gastric tumor (T) and adjacent non-tumor tissues (N) with progressive stages from T1 to T4.

Fig 3: Iterations of probe FAP2 to determine a sequence specific for FAP over PREP. (A) The structure of FAP2 probes and the different variations in the amino acid X; (B) Assessment of the probes (5µM) against rhFAP showing relative intensity and Talabostat (Tal) inhibiting cleavage. FAP2CD-Ser shows highest rate of cleavage; (C) Assessment of the probes (5µM) against rhPREP showing iteration FAP2AAla is cleaved by rhPREP; (D) Assessment of the probes against DPP-IV (5µM) showing none of the iterations are cleaved; (E) Comparison of the relative fluorescence intensity of each probe iteration at 60 minutes; (F) Comparison of FAP1Li-FAM with FAP2CD-Ser showing the signal intensity of FAP1 is higher within 90 minutes. Representative images, n=3, mean RFU plotted in bar graph and error bars show standard deviation.

Fig 4: FAP3 assessment against FAP-sP demonstrates FAP specificity. (A) The structure of FAP3; (B) The structure of FAP-sp; (C) Assessment of FAP3 and FAP-sP (all 5µM) when compared to prior iterations.; (D) rhPREP assessment demonstrating no rhPREP cleavage for FAP3 or FAP-sP; (E) DPPIV assessment demonstrating no exopeptidase activity. Representative plots, n=3 for each experiment run in triplicate.

Fig 5: High ATF4 levels correlate with increased COL1 expression on human melanoma and pancreatic tumours.a, Pearson correlation between the ISR target signature and COL1A2, FAP and PDGFRß in Skin Cutaneous Melanoma (SKCM) and Pancreatic adenocarcinoma (PAAD). The linear regression lines along with 95% confidence intervals (shaded regions) are shown. b, Pearson correlation between randomly selected genes and COL1A1 in Skin Cutaneous Melanoma (SKCM) and Pancreatic adenocarcinoma (PAAD). The linear regression lines along with 95% confidence intervals (shaded regions) are shown. c, Representative images from immunohistochemical staining for COL1 and ATF4 of human melanoma tissues d, Pearson correlation between the % ATF4 area and % COL1 area in primary group from the human melanoma tissue array. e, Pancreatic adenocarcinoma tissue array containing sections from 24 tumours were stained for COL1 (upper panel) and ATF4 (lower panel) proteins. f, Representative images from immunohistochemical staining for COL1 and ATF4 of human pancreatic adenocarcinoma tissues. g, Pearson correlation between the % ATF4 area and % COL1 area in all samples from e and h, in grade 2 (left) and grade 3 (right) groups. Source data