Fig 1: SPL enzyme is significantly upregulated in HD. a Western blot results indicate that SPL levels are significantly higher in STHdh Q111 cells compared to STHdh Q7 cells (p < 0.01, two-tailed t-test, 4 replicates). b Western blot experiments show a significant decrease in the Sgpl1-encoded protein after the knock-down experiments (p = 0.0015, two-tailed t-test, 3 replicates). The plot shows the levels of Sgpl-1 encoded protein. siRNA-mediated knock-down experiments were performed to validate the specificity of the SPL antibody (Abcam, ab56183): STHdh Q111 cells treated with siRNA against Sgpl1 (Sgpl1 KD) showed a drop in the signal compared to STHdh Q111 cells treated with negative control siRNA (Neg CTR). c Western blot analyses reveal that SPL enzyme levels are significantly upregulated (p < 0.001, two-tailed t-test, 8 control, and 12 HD samples) in postmortem cortical tissues of HD brain compared to controls. The boxplots represent the distribution of the ratios of SPL to actin levels, in which the first and the third quartiles of the data are displayed by the top and the bottoms of respective boxes and the median (second quartile value) is show as a horizontal line within the box