Fig 1: Co-localization of iNOS, Iba-1, and GFAP in MCAO mouse brain section. The mice were sacrificed 24 h after MCAO. Immunofluorescence stain was used to test the co-localization of iNOS, Iba-1, and GFAP in the MCAO mouse brain section. The iNOS (green) was mainly expressed in the infarct area and not expressed in the healthy area in the contralateral hemisphere. The iNOS protein was co-localized with GFAP (blue), but not with Iba-1 (red). Antibody: mouse anti-iNOS (1:200, Cat#ab49999, Abcam), anti-mouse Alex 488 conjugated secondary antibody (1:200, Cat#ab150117, Abcam), goat anti-GFAP (1:200, Cat#ab53554, Abcam), anti-goat Alex 647 conjugated secondary antibody (1:200, Cat#ab150135, Abcam), rabbit anti-Iba-1 (1:400, Cat#016-20001, Wako), anti-rabbit Alex 594 conjugated secondary antibody (1:200, Cat#ab150064, Abcam)