Anti-CDKN2A/p16INK4a antibody [2D9A12] ab54210 from Abcam

Product Specs
ItemAnti-CDKN2A/p16INK4a antibody [2D9A12]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab54210
Antibody TypePrimary
Clone2D9A12
IsotypeIgG2b
ClonalityMonoclonal
Quantity100 UL
HostMouse, Nude mouse
ApplicationsFlow Cytometry, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Conjugate/TagUnconjugated
Research AreaEpigenetics
Gene NameCDKN2A
ReactivityHuman, Rat
TargetCDKN2A/p16INK4a

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Clearance of defective muscle stem cells by senolytics restores myogenesis in myotonic dystrophy type 1Nature CommunicationsJuly 19, 2023Talita C. Conte, Gilberto Duran-Bishop, Zakaria Orfi, Inès Mokhtari, Alyson Deprez, Isabelle Côté, Thomas Molina, Tae-Yeon Kim, Lydia Tellier, Marie-Pier Roussel, et al.diluted in blocking solution: PAX7 (clone PAX7, 1:20; Developmental Studies Hybridoma Bank, DSHB), P16 (ab54210, 1:50; Abcam), KI67 (14-5698-82, clone SolA15, 1:500; Invitrogen), Myosin Heavy Chain (MF-20, 1:10;

(2) A novel multifunctional radioprotective strategy using P7C3 as a countermeasure against ionizing radiation-induced bone lossBone researchJune 29, 2023Wei F, Tuong ZK, Omer M, Ngo C, Asiatico J, Kinzel M, Pugazhendhi AS, Khaled AR, Ghosh R, Coathup M.Anti-p21/CIP1/CDKN1A antibody (NB1001941) was purchased from Novus Biologicals, USA. Anti-CDKN2A/p16INK4a antibody (ab54210) was purchased from Abcam, USA. IR and P7C3 treatment on cancer cell activity To investigate the effect

(3) Progression of irradiated mesenchymal stromal cells from early to late senescence: Changes in SASP composition and anti‐tumour propertiesCell ProliferationJune 1, 2023Nicola Alessio, Mustafa Burak Acar, Tiziana Squillaro, Domenico Aprile, Şerife Ayaz‐Güner, Giovanni Di Bernardo, Gianfranco Peluso, Servet Özcan, Umberto Galderisias P53 (sc‐126) and P21CIP1 (sc‐397) (Santa Cruz Biotechnology, Santa Cruz, CA, USA) and P16INK4A (ab54210) (Abcam, Cambridge, UK). Immunoreactive signals were detected with a horseradish peroxidase‐conjugated

(4) Comparison of the effects of oxidative and inflammatory stresses on rat chondrocyte senescenceScientific reportsMay 11, 2023Yagi M, Endo K, Komori K, Sekiya I.tesque, Kyoto, Japan) for 10 min and then incubated overnight with primary antibodies against p16 (1:200, ab54210; Abcam), p21 (1:200), MMP-13 (1:200), ADAMTS-5 (1:500), MCP-1 (1:200), IL-6 (1:200), type I collagen

(5) Dasatinib Attenuates Fibrosis in Keloids by Decreasing Senescent Cell BurdenActa Dermato-VenereologicaApril 6, 2023Claudia C. Darmawan, Keunyoung Hur, Novi Kusumaningrum, Jin Ho Chung, Si-Hyung Lee, Je-Ho Munin a high-pressure cooker for 30 min. The slides were then incubated with rabbit anti-p16 antibody (ab54210; Abcam). The primary antibody was washed with PBS and incubated with a rabbit secondary antibody for

(6) A stress-induced cilium-to-PML-NB route drives senescence initiationNature communicationsApril 3, 2023Ma X, Zhang Y, Zhang Y, Zhang X, Huang Y, He K, Chen C, Hao J, Zhao D, LeBrasseur NK, et al.Proteintech; SUMO-1 (BML-PW8330-0025; dilution 1:1000 for western blotting) from Enzo Life Sciences. p16INK4a (ab54210; dilution 1:200 for immunohistochemistry) from Abcam; p16INK4a (SPC-775D; dilution 1:500 for western blotting

(7) Phase Ib Study of Ulixertinib Plus Gemcitabine and Nab-Paclitaxel in Patients with Metastatic Pancreatic AdenocarcinomaThe OncologistFebruary 8, 2023Patrick M Grierson, Benjamin Tan, Katrina S Pedersen, Haeseong Park, Rama Suresh, Manik A Amin, Nikolaos A Trikalinos, Deborah Knoerzer, Brent Kreider, Anupama Reddy, et al.for 60 minutes); anti-MYC (Abcam, Cat# ab32072, 1:75 for 60 minutes); and anti-p16INK4a (Abcam, Cat# ab54210, 1:4000 for 15 minutes). The antibody binding signals were detected using the Mach 3 Rabbit HRP Polymer Detection

(8) Proteogenomics of diffuse gliomas reveal molecular subtypes associated with specific therapeutic targets and immune-evasion mechanismsNature communicationsJanuary 31, 2023Wang Y, Luo R, Zhang X, Xiang H, Yang B, Feng J, Deng M, Ran P, Sujie A, Zhang F, et al.ab33889, dilution 1:500), anti-MKI67 (Abcam ab16667, dilution 1:500), Anti-CDKN2A/p16INK4a antibody (Abcam ab54210, dilution 1:500), anti-CDK4 (Abcam ab108357, dilution 1:500), anti-EGFR (Abcam ab52894, dilution 1:500),

(9) Eldecalcitol prevented OVX-induced osteoporosis through inhibiting BMSCs senescence by regulating the SIRT1-Nrf2 signalFrontiers in pharmacologyJanuary 1, 2023Kou Y, Rong X, Tang R, Zhang Y, Yang P, Liu H, Ma W, Li M.diluted to the corresponding concentration. The Anti-Osterix antibody (ab209484), Anti-P16 antibody (ab54210) and secondary antibodies (ab6721, ab102448, ab150081, and ab150119) were purchased from Abcam (Cambridge,

(10) Characterization of cellular senescence in radiation ulcers and therapeutic effects of mesenchymal stem cell-derived conditioned mediumBurns & traumaJanuary 1, 2023Chen W, Wang Y, Zheng J, Chen Y, Zhang C, Yang W, Wu L, Yang Z, Wang Y, Shi C.blocking solution for 40 min at room temperature and then with primary antibodies against p16 (Abcam, ab54210), p21 (Santa Cruz, sc817), Ki67 (Abcam, ab15580), phosphorylated histone H2AX (γ-H2AX; Abcam, ab81299),

(11) Reduction in Lens Epithelial Cell Senescence Burden through Dasatinib plus Quercetin or Rapamycin Alleviates D-Galactose-Induced Cataract ProgressionJournal of Functional BiomaterialsDecember 21, 2022Yinhao Wang, Yulin Tseng, Keyu Chen, Xinglin Wang, Zebin Mao, Xuemin Liserum at room temperature (20–25 °C) for 20 min and incubated with primary anti-p16 (Abcam, Cat. No. ab54210, Cambridge, UK, 1:10,000) and anti-gamma H2A.X (γH2AX; Abcam, Cat. No. ab81299, Cambridge, UK, 1:250)

(12) Toll-like receptor 2 orchestrates a tumor suppressor response in non-small cell lung cancerCell reportsNovember 8, 2022Millar FR, Pennycuick A, Muir M, Quintanilla A, Hari P, Freyer E, Gautier P, Meynert A, Grimes G, Coll CS, et al.#556431; RRID:AB_396415 Rat anti-p19/Arf,Abcam,Cat #Ab174939; RRID:AB_2924683 Mouse anti-p16,Abcam,Cat #Ab54210; RRID:AB_881819 Goat anti-IL-1 α,R&D systems,Cat #AF-400; RRID:AB_354473 Rabbit anti-IL-1 β,Santa Cruz

(13) A BDNF-TrkB autocrine loop enhances senescent cell viabilityNature CommunicationsOctober 20, 2022Carlos Anerillas, Allison B. Herman, Rachel Munk, Amanda Garrido, Kwan-Wood Gabriel Lam, Matthew J. Payea, Martina Rossi, Dimitrios Tsitsipatis, Jennifer L. Martindale, Yulan Piao, et al.(EPR1292, Abcam, ab108319; 1:100 dilution in blocking solution) and CDKN2A/p16INK4a (2D9A12, Abcam, ab54210; 1:250 dilution in blocking solution). Fluorescent secondary antibodies (Invitrogen) were added in 10%

(14) Captopril Alleviates Chondrocyte Senescence in DOCA-Salt Hypertensive Rats Associated with Gut Microbiome AlterationCellsOctober 10, 2022Lok Chun Chan, Yuqi Zhang, Xiaoqing Kuang, Mohamad Koohi-Moghadam, Haicui Wu, Theo Yu Chung Lam, Jiachi Chiou, Chunyi Wenwith the primary antibody overnight at 4 °C, where the primary antibodies used were p16 (1:500; Abcam, ab54210), p53, and MMP13 antibody (1:500; Abcam, ab39012). For 3,3’-Diaminobenzidine (DAB) staining, we used

(15) Establishment of In Vitro Models by Stress-Induced Premature Senescence for Characterizing the Stromal Vascular Niche in Human Adipose TissueLife (Basel)September 20, 2022Marlene Wahlmueller, Marie-Sophie Narzt, Karin Missfeldt, Verena Arminger, Anna Krasensky, Ingo LÃ¤mmermann, Barbara Schaedl, Mario Mairhofer, Susanne Suessner, Susanne Wolbank, et al.primary antibodies recombinant anti-p21 ab109520 (1:100; Abcam, Cambridge, UK), anti-CDKN2A/p16INK4a ab54210 (1:1000; Abcam), anti-Lamin B1 ab16048 (1:250; Abcam), anti-CD31 (1:20; Dako, M0823, Clone JC70A), anti-CD34

(16) mtDNA variability determines spontaneous joint aging damage in a conplastic mouse modelAging (Albany NY)August 15, 2022Morena Scotece, Carlos Vaamonde-García, Ana Victoria Lechuga-Vieco, Alberto Centeno Cortés, María Concepción Jiménez Gómez, Purificación Filgueira-Fernández, Ignacio Rego-Pérez, José Antonio Enríquez, Francisco J. Blancofor 1 h at room temperature, or Ki67 (ab 15580; Abcam; 1:400), P62 (MAB8028;R&D Systems; 1:200), p16 (ab54210; Abcam; 1:200) overnight at 4° C, followed by use of the Dako REAL™ EnVision™ Detection System, Peroxidase/DAB+

(17) Senolytic drugs relieve pain by reducing peripheral nociceptive signaling without modifying joint tissue damage in spontaneous osteoarthritisAging (Albany NY)August 15, 2022Tae-Hwan Gil, Haiyan Zheng, Hyo Gyeong Lee, Ji-Won Shin, Sun Wook Hwang, Ki-Mo Jang, Ok Hee JeonUSA) was used per the manufacturer’s protocol. Primary antibodies against p16INK4a (1:250, Abcam, #ab54210, USA) and HMGB1 (1:1000, Abcam, #ab18256, USA) were incubated overnight at 4° C, followed by incubation

(18) Single-cell transcriptomics reveals a senescence-associated IL-6/CCR6 axis driving radiodermatitisEMBO molecular medicineAugust 8, 2022Paldor M, Levkovitch-Siany O, Eidelshtein D, Adar R, Enk CD, Marmary Y, Elgavish S, Nevo Y, Benyamini H, Plaschkes I, et al.anti‐phospho‐ERK1/2 (M8159, 1:4,000; Sigma), PLAUR/uPAR antibody (orb13650, 1:300; Biorbyt Ltd.), anti‐p16INK4A (ab54210, 1:100; Abcam), and β‐actin antibody (A5316, 1:10,000; Sigma). Immunostainings and western blots were

(19) A motor neuron disease mouse model reveals a non-canonical profile of senescence biomarkersDisease models & mechanismsAugust 1, 2022Torres P, Anerillas C, Ramírez-Núñez O, Fernàndez A, Encinas M, Povedano M, Andrés-Benito P, Ferrer I, Ayala V, Pamplona R, et al.blocked with 5% bovine serum albumin in PBS for 1 h at room temperature. The primary antibodies anti-p16 (ab54210, Abcam; 1:100) and anti-GFAP (ab7260, Abcam; 1:200) or anti-Iba1 (019-19741, FUJIFILM Wako; 1:200) were

(20) Validation of a Patient-Derived Xenograft Model for Cervical Cancer Based on Genomic and Phenotypic CharacterizationCancers (Basel)June 16, 2022Shunsuke Miyamoto et al.were prepared using standard procedures and stained with H&E. For immunohistochemical analysis, P16 (ab54210, 1:100 dilution, Abcam, Cambridge, MA, USA) and Ki-67 (M7240, 1:100 dilution, Dako Japan, Tokyo, Japan)

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Anti-CDKN2A/p16INK4a antibody [2D9A12] from Abcam

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Anti-CDKN2A/p16INK4a antibody [2D9A12] from Abcam

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