Anti-Cofilin antibody ab42824 from Abcam

Product Specs
ItemAnti-Cofilin antibody
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab42824
Antibody TypePrimary
ApplicationsImmunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
IsotypeIgG
ClonalityPolyclonal
Quantity100 UG
HostRabbit
Conjugate/TagUnconjugated
Concentration1 mg/ml
Gene NameCFL1
ReactivityHuman, Mouse, Rat
TargetCofilin

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) miR-137-LAPTM4B regulates cytoskeleton organization and cancer metastasis via the RhoA-LIMK-Cofilin pathway in osteosarcomaOncogenesisMay 6, 2023Yan R, Liu D, Wang J, Liu M, Guo H, Bai J, Yang S, Chang J, Yao Z, Yang Z, et al.(Cat#PRB-440P), Cofilin (Phospho S3) antibody from Abcam (Cat#ab12866), Cofilin antibody from Abcam (Cat#ab42824), Phospho-LIMK1(Thr508)/LIMK2(Thr505) antibody from Cell Signaling Technology (Cat#3841), Flag antibody

(2) High-content synaptic phenotyping in human cellular models reveals a role for BET proteins in synapse assemblyeLifeApril 21, 2023Berryer MH, Rizki G, Nathanson A, Klein JA, Trendafilova D, Susco SG, Lam D, Messana A, Holton KM, Karhohs KW, et al.,Thermo Fisher Scientific,Cat#PA5-30386 RRID: AB_2547860,WB (1:500) antibody,Anti-human COFILIN,Abcam,Cat#ab42824 RRID: AB_879739,WB (1:1000) recombinant DNA reagent,pAAVS1-iNGN2-Zeo (plasmid),This study,, sequence-based

(3) Identification of Cofilin‐1 as a novel biomarker of atopic dermatitis using iTRAQ quantitative proteomicsJournal of Clinical Laboratory AnalysisNovember 1, 2022Xiaotao Zhou, Bo Xiao, Jiajia Zeng, Liying Zhou, Xiaodong Wang, Shangqi Zhao, Xiaobo Li, Huiqiu Zhang, Yanjun Su, Zhenyu Zhao, et al.formaldehyde‐fixed and paraffin‐embedded samples were dewaxed and rehydrated. Rabbit anti‐human CFL1 antibody (ab42824; Abcam) was applied, followed by SP staining. IHC assay were performed as described previously. 15 2.6

(4) LMO3 promotes proliferation and metastasis of papillary thyroid carcinoma cells by regulating LIMK1-mediated cofilin and the β-catenin pathwayOpen Med (Wars)March 7, 2022Zeyi Ling, Xiaoli Long, Ying Wu, Jie Li, Mingliang FengAbcam); anti-cleaved caspase-3 (ab2302) and anti-cleaved PARP (ab4830) (1:3,500; Abcam); anti-cofilin (ab42824) and anti-p-cofilin (ab12866) (1:4,000; Abcam); anti-β-catenin (ab6302), anti-β-tubulin (ab6046), and

(5) Cervical Cancer Cells-Derived Extracellular Vesicles Containing microRNA-146a-5p Affect Actin Dynamics to Promote Cervical Cancer Metastasis by Activating the Hippo-YAP Signaling Pathway via WWC2Journal of oncologyJanuary 1, 2022Wang W, Wu L, Tian J, Yan W, Qi C, Liu W, Xuan S, Shang A.phosphorylated (p)-LATS1 (1 : 1000, #8654, CST), p-YAP (1 : 1000, #4911, CST), cofilin (1.0 μg/mL, ab42824, Abcam), p-cofilin (1 : 1000, ab12866, Abcam), F-actin (1 : 1000, ab205, Abcam), G-actin (1 : 1000,

(6) hnRNP Q and hnRNP A1 Regulate the Translation of Cofilin in Response to Transient Oxygen–Glucose Deprivation in Hippocampal NeuronsCellsDecember 17, 2021Sung Wook Kim, In Kyung Hong, Mingee Kim, Yun Seon Song, Kyong-Tai KimSigma-Aldrich, R5653), Anti-hnRNP A1 (1:1000; Santa Cruz, sc-32301), Anti-Cofilin (1:250; Abcam, ab54532, ab42824), Anti-14-3-3ζ (1:1000; Santa Cruz, sc-1657, sc-1019), Anti-GAPDH (1:1000; Bethyl, A300-641A), normal

(7) iTRAQ-based proteomic analysis of sperm reveals candidate proteins that affect the quality of spermatozoa from boars on plateausProteome ScienceDecember 1, 2021Yanling Zhao, Yaomei Wang, Feipeng Guo, Bo Lu, Jiale Sun, Jianzhou Wang, Zili RenBeyotime Biotechnology Co. Ltd, Shanghai, China), incubated with the appropriate primary antibody (1:1000, ab42824, ab231103, ab32419, ab231174 or ab8227, Abcam, Cambridge, UK) and gently shaken at room temperature

(8) Environmental enrichment combined with fasudil promotes motor function recovery and axonal regeneration after strokeNeural regeneration researchDecember 1, 2021Zhu YT, Zhang Q, Xie HY, Yu KW, Xu GJ, Li SY, Wu Y.rabbit anti-p-cofilin (phosphor S3; Cat# ab12866, 1:500, Abcam, Cambridge, MA, USA), anti-cofilin (Cat# ab42824, 1:2000, Abcam), anti-p-LIM domain kinase (LIMK)1 (phosphor T508; Cat# ab38508, 1:1000, Abcam), anti-LIMK1

(9) High stretch induces endothelial dysfunction accompanied by oxidative stress and actin remodeling in human saphenous vein endothelial cellsScientific ReportsDecember 1, 2021T. Girão-Silva, M. H. Fonseca-Alaniz, J. C. Ribeiro-Silva, J. Lee, N. P. Patil, L. A. Dallan, A. B. Baker, M. C. Harmsen, J. E. Krieger, A. A. Miyakawano. Staining dilution WB dilution MW (KDa) Calponin Millipore/04-589 1:100 1:500 34 Cofilin Abcam/ab42824 1:100 – 19 Collagen I Abcam/ab34710 1:500 – 115 Deoxyribonuclease I (G-actin) AF488 Life/D12371 1:200 – – GAPDH Abcam/ab9485 – 1:5000 37 PECAM1 Abcam/ab24590 1:100 1:1000 130 pFAK

(10) Vascular smooth muscle cell dysfunction contribute to neuroinflammation and Tau hyperphosphorylation in Alzheimer diseaseiScienceSeptember 24, 2021Aguilar-Pineda JA, Vera-Lopez KJ, Shrivastava P, Chávez-Fumagalli MA, Nieto-Montesinos R, Alvarez-Fernandez KL, Goyzueta Mamani LD, Davila Del-Carpio G, Gomez-Valdez B, Miller CL, et al.(Santa Cruz biotechnology, sc-73614), anti-VCL (Abcam, ab254410) and anti-p-Tau Y18 (GeneTex, GTX54658), Anti-CFL-1 (Abcam, ab42824). Immunoblot uncut membranes are provided in DATA S1. Computational details System building, structural

(11) Mirtronic miR-4646-5p promotes gastric cancer metastasis by regulating ABHD16A and metabolite lysophosphatidylserinesCell death and differentiationSeptember 1, 2021Yang L, Hou Y, Du YE, Li Q, Zhou F, Li Y, Zeng H, Jin T, Wan X, Guan S, et al.anti-LIMK1 (1:1000; ab119084, Abcam), anti-p-LIMK1 (1:1000; ab38508, Abcam), anti-cofilin (1:1000; ab42824, Abcam), anti-p-cofilin (1:1000; ab12866, Abcam) and anti-β-Actin (1:1000; Bioshap). Next, the membranes

(12) MiR-200c-3p Modulates Cisplatin Resistance in Biliary Tract Cancer by ZEB1-Independent MechanismsCancers (Basel)August 8, 2021Florian Posch et al.verify equal protein loading, membranes were stripped and re-probed for Cofilin (Anti-Cofilin antibody, ab42824, Abcam, Cambridge, UK; diluted 1:5000 in 2.5% M-TBS-T). Uncropped Western blot images can be found at

(13) The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviourCellular and molecular life sciences : CMLSApril 1, 2021Castroflorio E, den Hoed J, Svistunova D, Finelli MJ, Cebrian-Serrano A, Corrochano S, Bassett AR, Davies B, Oliver PL.anti-LAMP1 (1:1000 Abcam ab24170), anti-Rab7 (1:1000, Abcam ab137029), anti-Cofilin (1:1000, Abcam ab42824), anti-TBC1D24 (1:1000, Proteintech 25254-1-AP) and anti-OXR1 (1:1000, Proteintech 13514-1-AP). Proteins

(14) The common YAP activation mediates corneal epithelial regeneration and repair with different-sized woundsNPJ Regenerative medicineMarch 26, 2021Li Y, Ge L, Chen X, Mao Y, Gu X, Ren B, Zeng Y, Chen M, Chen S, Liu J, et al.Ki67 (Abcam, ab15580; 1:800), rabbit p-Cofilin (Ser3; Abcam, ab12866; 1:2000), rabbit Cofilin (Abcam, ab42824; 1:2500), rabbit p-LIMK (LIMK1 Thr 508, LIMK2 Thr 505; CST, 3841S; 1:1000), rabbit LIMK (CST, 3842S;

(15) Cerebral Ischemia-Reperfusion Is Associated With Upregulation of Cofilin-1 in the Motor CortexFrontiers in Cell and Developmental BiologyMarch 11, 2021Ming-Shu Xu, Lei-Miao Yin, Ai-Fang Cheng, Ying-Jie Zhang, Di Zhang, Miao-Miao Tao, Yun-Yi Deng, Lin-Bao Ge, Chun-Lei Shantemperature for 30 min, the excess liquid was removed. The primary antibody (anti-cofilin-1 primary antibody, ab42824, Abcam Inc., 1:100; anti drebrin primary antibody, ab11068, Abcam Inc., 1:50; AntiARPC2 antibody, HPA008352,

(16) Differential proteomic analysis of children infected with respiratory syncytial virusBrazilian Journal of Medical and Biological ResearchFebruary 26, 2021Gen-Quan Yin et al.2 (PRDX2; ab109367, Abcam), bisphosphoglycerate mutase (BPGM; ab97497, Abcam), and cofilin 1 (CFL1; ab42824, Abcam) at 4°C overnight. The next day, membranes were washed three times in TBST and incubated with

(17) Neuroligin1 Contributes to Neuropathic Pain by Promoting Phosphorylation of Cofilin in Excitatory NeuronsFrontiers in Molecular NeuroscienceFebruary 25, 2021Junlin Ouyang, Xiaping Chen, Shanchun Su, Xiaohui Li, Xueqin Xu, Xinhua Yu, Changbin Ke, Xiaohu Zhurabbit anti-phospho-cofilin (1:1000, 3313, Cell Signaling Technology), mouse anti-cofilin (1:1000, ab42824, Abcam), rabbit antiGluR1 (1:1000, AB1504, Millipore), rabbit anti-GAPDH (1:1000, 2118, Cell Signaling Technology

(18) Unraveling the Molecular Tumor-Promoting Regulation of Cofilin-1 in Pancreatic CancerCancers (Basel)February 10, 2021Silke D. Werle et al.4–6 h at 4 ◦ C in 1×TBS, 0.1% Tween 20 (TBS-T) and 5% powdered milk. Primary antibodies (anti-CFL1 #ab42824, Abcam, Cambridge, UK; anti-Caspase-3 #9662, Cell Signaling Technology, Danvers, MA, USA; anti-Cleaved

(19) Diacylglycerol kinase and phospholipase D inhibitors alter the cellular lipidome and endosomal sorting towards the Golgi apparatusCellular and molecular life sciences : CMLSFebruary 1, 2021Lingelem ABD, Kavaliauskiene S, Halsne R, Klokk TI, Surma MA, Klose C, Skotland T, Sandvig K.rabbit anti-Phospho-(Ser/Thr) PKD substrate (4381, Cell Signaling Technology), rabbit anti-cofilin (ab42824, Abcam), goat anti-Vps35 (ab10099, Abcam), mouse anti-SNX2 (611308, BD Transduction Laboratories). Secondary

(20) Environmental enrichment combined with fasudil treatment inhibits neuronal death in the hippocampal CA1 region and ameliorates memory deficitsNeural Regeneration ResearchJanuary 12, 2021Gao-Jing Xie, Qun Zhang, Gao-Jing Xu, Yi-Tong Zhang, Si-Yue Li, Chuan-Jie Wang, Hong-Yu Xie, Chuan-Jie Wangincubated overnight at 4°C with the following primary antibodies: anti-cofilin antibody (1:1000; rabbit; ab42824, Abcam, Cambridge, MA, USA), anti-cofilin (phospho S3) antibody (1:1000; rabbit, ab12866, Abcam), and

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Anti-Cofilin antibody from Abcam

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Anti-Cofilin antibody from Abcam

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