Anti-MAPRE1/EB1 antibody [KT51] ab53358 from Abcam

Product Specs
ItemAnti-MAPRE1/EB1 antibody [KT51]
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab53358
Antibody TypePrimary
IsotypeIgG2a
Quantity100 UG
ClonalityMonoclonal
ApplicationsFlow Cytometry, Western blot
CloneKT51
HostRat
Conjugate/TagUnconjugated
Concentration1 mg/ml
Gene NameMAPRE1
ReactivityHuman
TargetMAPRE1

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Kinetochore-microtubule attachment in human cells is regulated by the interaction of a conserved motif of Ska1 with EB1The Journal of biological chemistryFebruary 1, 2023Radhakrishnan RM, Kizhakkeduth ST, Nair VM, Ayyappan S, Lakshmi RB, Babu N, Prasannajith A, Umeda K, Vijayan V, Kodera N, et al.antibody of Ska1 (Cat # ab118586), Ska3 (Cat # ab186003), and rat monoclonal antibody of EB1 (Cat # ab53358) were obtained from Abcam. Rabbit polyclonal antibody for Ska1 (NBP1-72131) was obtained from Novus Biologicals

(2) Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammalsCell reportsApril 5, 2022Almeida AC, Soares-de-Oliveira J, Drpic D, Cheeseman LP, Damas J, Lewin HA, Larkin DM, Aguiar P, Pereira AJ, Maiato H.anti-CLIP-170,"Gift from N. Galjart (Molines et al., 2020)",N/A Rat monoclonal anti-EB1,Abcam,"Cat# ab53358, RRID:AB_881313" Rat monoclonal anti-EB3,"Gift from A. Akhmanova (Stepanova et al., 2003)",N/A Mouse

(3) Decreased tubulin-binding cofactor B was involved in the formation disorder of nascent astrocyte processes by regulating microtubule plus-end growth through binding with end-binding proteins 1 and 3 after chronic alcohol exposureFrontiers in cellular neuroscienceJanuary 1, 2022Zheng Y, Yang M, Chen X, Zhang G, Wan S, Zhang B, Huo J, Liu H.anti-TBCB, 1:250, sc-377139, Santa Cruz, USA; Anti-MAPRE1, 1:1,000, NHA2107, Novogene, China; Anti-MAPRE1, ab53358, Abcam, UK; Anti-MAPRE3, 1:1,000, #AP52003-100, Abcepta, China; anti-α-T, 1: 5,000, GTX628802, GeneTex,

(4) CLIP-170S is a microtubule +TIP variant that confers resistance to taxanes by impairing drug-target engagementDevelopmental cellDecember 6, 2021Thakkar PV, Kita K, Castillo UD, Galletti G, Madhukar N, Navarro EV, Barasoain I, Goodson HV, Sackett D, Díaz JF, et al.antibody (YL1/2 clone),Bio-rad,"Cat No: MCA77G, RRID:AB_325003" EB-1 antibody (KT51 clone),Abcam,"Cat No: ab53358, RRID:AB_881313" Mouse anti-Myc antibody (9E10 clone),Santacruz biotechnology,"Cat No: sc-40, RRID:AB_2857941" Bacterial

(5) Delayed KCNQ1/KCNE1 assembly on the cell surface helps I(Ks) fulfil its function as a repolarization reserve in the heartThe Journal of physiologyJuly 1, 2021Wilson ZT, Jiang M, Geng J, Kaur S, Workman SW, Hao J, Bernas T, Tseng GN.(ab5450, AbCam), GFP rabbit Ab (ab290, AbCam), GFP mouse Ab (MA5-15256, Thermo Fisher), and EB1 rat Ab (ab53358, AbCam). Alexa-conjugated secondary Abs and WGA were purchased from Molecular Probes. HRP-conjugated

(6) Nonredundant roles of DIAPHs in primary ciliogenesisThe Journal of biological chemistryJanuary 1, 2021Palander O, Lam A, Collins RF, Moraes TJ, Trimble WS.(ab214317), Polycystin1 (ab235963 or ab74115), GFP (ab13970), Arf4 (ab171746), EB3 (ab53360), and EB1 (ab53358) were obtained from Abcam, Cambridge, Massachusetts, United States. BBS1 (21118-1-AP), Arl13B (1711-1-AP),

(7) Distinct retrograde microtubule motor sets drive early and late endosome transportThe EMBO journalDecember 15, 2020Villari G, Enrico Bena C, Del Giudice M, Gioelli N, Sandri C, Camillo C, Fiorio Pla A, Bosia C, Serini G.anti‐KIF5B ab167429 [EPR10276], and monoclonal mouse Ab anti‐mCherry ab125096 [1C51] and rat anti‐EB1 ab53358 [KT51] and mouse polyclonal Ab anti‐HOOK3 ab173388 as well as the rabbit polyclonal anti‐FLAG ab1162

(8) MiR-93 is related to poor prognosis in pancreatic cancer and promotes tumor progression by targeting microtubule dynamicsOncogenesisMay 4, 2020Elena Vila-Navarro et al.anti-CDK1 (1:10,000; ab133327, Abcam), anti-CHMP4B (1:250; HPA041401, Sigma-Aldrich), anti-MAPRE1 (1:5000; ab53358, Abcam), anti-MAD2L1 (1:1000; ab97777, Abcam), anti-YES1 (1:5000; ab109265, Abcam), anti-Ki67 (1:5000;

(9) A Novel Microtubule-Tau Association Enhancer and Neuroprotective Drug Candidate: Ac-SKIPFrontiers in Cellular NeuroscienceOctober 1, 2019Yanina Ivashko-Pachima, Illana Gozesmanufacture protocol (Pierce, Rockford, IL, United States). Briefly, 10 µg of antibodies of interest (EB1, ab53358, Abcam, Cambridge, United Kingdom; and total Tau antibody, AT-5004, MBL, Billerica, MA, United States) were

(10) The Golgi Outpost Protein TPPP Nucleates Microtubules and Is Critical for MyelinationCellSeptember 19, 2019Fu MM, McAlear TS, Nguyen H, Oses-Prieto JA, Valenzuela A, Shi RD, Perrino JJ, Huang TT, Burlingame AL, Bechstedt S, et al.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies "Anti-EB1 (Clone KT51), rat monoclonal",Abcam,Cat#ab53358 "Anti-GFP (Clone JL-8), mouse monoclonal",Takara Clontech,Cat#632381 "Anti-GM130 (Clone 35), mouse

(11) LRRC45 contributes to early steps of axoneme extensionJournal of cell scienceSeptember 20, 2018Kurtulmus B, Yuan C, Schuy J, Neuner A, Hata S, Kalamakis G, Martin-Villalba A, Pereira G.polyclonal anti-FBF1 (1:500, Sigma Aldrich, #HPA023677); rabbit polyclonal anti-MAPRE1 (EB1) (1:300, Abcam, #ab53358); rabbit polyclonal anti-CP110 (1:300, Bethyl Laboratories, #A301-343A). RNA interference Transfections

(12) EB1 binding restricts STIM1 translocation to ER–PM junctions and regulates store-operated Ca2+ entryJournal of Cell BiologyJune 4, 2018Chi-Lun Chang, Yu-Ju Chen, Carlo Giovanni Quintanilla, Ting-Sung Hsieh, Jen LiouAP20187 was purchased from Takara Bio Inc. BHQ was obtained from EMD Millipore. Anti-EB1 antibody (ab53358) and anti–β actin antibody (ab8227) were obtained from Abcam. Anti-GAPDH antibody (G-8795) was purchased

(13) Sodium Channel Remodeling in Subcellular Microdomains of Murine Failing CardiomyocytesJournal of the American Heart AssociationDecember 8, 2017Mathilde R. Rivaud, Esperanza Agullo‐Pascual, Xianming Lin, Alejandra Leo‐Macias, Mingliang Zhang, Eli Rothenberg, Connie R. Bezzina, Mario Delmar, Carol Ann Remmeα‐tubulin (sc‐5286; Santa Cruz Biotechnology, Dallas, TX, USA), End‐binding protein 1 (EB1) (MAPRE1; ab53358; Abcam, Cambridge, UK), connexin 43 (610061; BD Transduction Laboratories, San Jose, CA, USA), calnexin

(14) HMMR acts in the PLK1-dependent spindle positioning pathway and supports neural developmenteLifeOctober 10, 2017Connell M, Chen H, Jiang J, Kuan CW, Fotovati A, Chu TL, He Z, Lengyell TC, Li H, Kroll T, et al.MA)",3624 antibody,mCherry,Abcam,ab167453 antibody,DIC,"Millipore (Burlington, MA)",mab1618 antibody,DynLL1,Abcam,ab51603 antibody,EB1,Abcam,ab53358 antibody,gamma-tubulin,Sigma,t6557 antibody,GFP,Abcam,ab1218 antibody,HMMR,Abcam,ab124729 antibody,pHMMR,PMID:22110403, antibody,NuMA,Abcam,ab36999 antibody,NuMA,Cell Signaling Technology

(15) EB1 regulates attachment of Ska1 with microtubules by forming extended structures on the microtubule latticeNature CommunicationsSeptember 1, 2016Geethu E. Thomas et al.antibodies of Ska1 (Cat # ab118586), Ska2 (Cat # ab91551), and rat monoclonal antibody of EB1 (Cat # ab53358) were obtained from Abcam (Cambridge, MA, USA). Mouse monoclonal antibody against α-tubulin (Cat # T6199)

(16) The γ-tubulin-specific inhibitor gatastatin reveals temporal requirements of microtubule nucleation during the cell cycleNature CommunicationsDecember 1, 2015Takumi Chinen et al.0.5% bovine serum albumin, cells were incubated with anti-EB1 antibody (1:500 dilution, Abcam, Cat# ab53358), anti-α-tubulin antibody (1:500 dilution, Sigma, Cat# T9026) and anti-pericentrin antibody (1:2,000

(17) Nek5 promotes centrosome integrity in interphase and loss of centrosome cohesion in mitosisJournal of Cell BiologyMay 11, 2015Suzanna L. Prosser, Navdeep K. Sahota, Laurence Pelletier, Ciaran G. Morrison, Andrew M. FryEMD Millipore), and α-tubulin (T6199, 1:1,000; Sigma-Aldrich). Rat monoclonal antibody was used against EB1 (ab53358, 1:250; Abcam). Rabbit polyclonal antibodies were used against Nek5 (this study, 1:200), C-Nap1 (raised

(18) The novel actin/focal adhesion-associated protein MISP is involved in mitotic spindle positioning in human cellsCell cycle (Georgetown, Tex.)May 1, 2013Maier B, Kirsch M, Anderhub S, Zentgraf H, Krämer A.antiserum against Crest (ANA, Euroimmun, CA1611-0101) and a rat monoclonal antibody against EB1 (Abcam, ab53358). A sheep polyclonal antibody against BubR1 was kindly provided by S.S. Taylor. Immunofluorescence microscopy

(19) Cdk-mediated phosphorylation of the Kvβ2 auxiliary subunit regulates Kv1 channel axonal targetingThe Journal of cell biologyMarch 7, 2011Vacher H, Yang JW, Cerda O, Autillo-Touati A, Dargent B, Trimmer JS.

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Anti-MAPRE1/EB1 antibody [KT51] from Abcam

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Anti-MAPRE1/EB1 antibody [KT51] from Abcam

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