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Antibodies
Anti-DCAMKL1 antibody
Anti-DCAMKL1 antibody from Abcam
Be the first to write a review!
Citations:
(108)
Supplier Page
Supplier Page from
Abcam for
Anti-DCAMKL1 antibody
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-DCAMKL1 antibody
Company
Abcam
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
ab31704
Antibody Type
Primary
Isotype
IgG
Clonality
Polyclonal
Applications
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western blot
Host
Rabbit
Quantity
100 UG
Conjugate/Tag
Unconjugated
Concentration
0.5 mg/ml
Gene Name
DCLK1
Validation
KnockOut Validated
Reactivity
Human, Mouse, Rat
Target
DCAMKL1
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (108)
(1)
Generation, culture, and stimulation of small intestinal murine organoids in parasitology research
STAR Protocols
December 1, 2023
Marta Campillo Poveda, Claire Drurey, Rick M. Maizels
REAGENT or RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,,
Anti-DCAMKL1
antibody,
Abcam
,
ab31704
"Goat anti-rabbit IgG (H + L) secondary antibody, FITC",
Invitrogen
,
65-6111
Fixable Viability Dye eFluor
(2)
Partial in vivo reprogramming enables injury-free intestinal regeneration via autonomous Ptgs1 induction
Science Advances
November 24, 2023
Jumee Kim, Somi Kim, Seung-Yeon Lee, Beom-Ki Jo, Ji-Young Oh, Eun-Ji Kwon, Keun-Tae Kim, Anish Ashok Adpaikar, Eun-Jung Kim, Han-Sung Jung,
et al.
1:100
), rabbit anti–E-cadherin (
Cell Signaling Technology
,
3195
,
1:1000
), and rabbit anti-Dclk1(
Abcam
,
ab31704
,
1:1000
).
Histology
and AB-PAS
staining
For histological analysis, sections were stained with hematoxylin
(3)
Sterile triggers drive joint inflammation in TNF‐ and IL‐1β‐dependent mouse arthritis models
EMBO Molecular Medicine
October 11, 2023
Alexandra Thiran, Ioanna Petta, Gillian Blancke, Marie Thorp, Guillaume Planckaert, Maude Jans, Vanessa Andries, Korneel Barbry, Elisabeth Gilis, Julie Coudenys,
et al.
with primary antibody (rabbit anti‐lysozyme (Dako; EC 3.2.1.17, 1/500) or rabbit
anti‐DCAMKL1
(
Abcam
;
Ab31704
, 1/500)) overnight at 4°C. After washing the slides with PBS, ileum sections were counterstained with
(4)
Single-Cell RNA Sequencing (scRNA-seq) Identifies L1CAM as a Key Mediator between Epithelial Tuft Cell and Innate Lymphoid Cell in the Colon of Hnrnp I Knockout Mice
Biomedicines
October 9, 2023
Guanying (Bianca) Xu, Yuan-Xiang Pan, Wenyan Mei, Hong Chen
the respective
procedures
. In brief, primary antibodies of anti-DCAMKL1/DCLK1 (Abcam, Cambridge, UK,
ab31704
, dilution at
1:200
), anti-L1CAM (
Abcam
, Cambridge, UK,
ab24345
, dilution at
1:100
), anti-
GATA3
(
Abcam
,
(5)
Proteomic investigation of neural stem cell to oligodendrocyte precursor cell differentiation reveals phosphorylation-dependent Dclk1 processing
Cellular and molecular life sciences : CMLS
August 18, 2023
Hardt R, Dehghani A, Schoor C, Gödderz M, Cengiz Winter N, Ahmadi S, Sharma R, Schork K, Eisenacher M, Gieselmann V,
et al
.
blocking solution: anti-myc-tag (
1:5000
, rabbit pAb,
ab9106
,
Abcam
, UK), anti-Dclk1 (
1:1000
, rabbit pAb,
ab31704
,
Abcam
, UK),
anti-Padi2
(
1:1000
, rabbit pAb,
12110-1-AP
,
Proteintech Group
, Rosemont, IL), anti-Pura
(6)
Diet-mediated constitutive induction of novel IL-4+ ILC2 cells maintains intestinal homeostasis in mice
Journal of Experimental Medicine
August 7, 2023
Wanlin Cui, Yuji Nagano, Satoru Morita, Takeshi Tanoue, Hidehiro Yamane, Keiko Ishikawa, Toshiro Sato, Masato Kubo, Shohei Hori, Tadatsugu Taniguchi,
et al.
(
103134
;
BioLegend
),
EPCAM
(
118214
;
BioLegend
) and DCLK1 (primary antibody, rabbit anti-DCLK1,
Abcam
ab31704
; secondary antibody, goat anti-rabbit IgG-AF488,
Life Technologies
). Tuft cells were defined as the
(7)
An organoid-based CRISPR-Cas9 screen for regulators of intestinal epithelial maturation and cell fate
Science advances
July 14, 2023
Hansen SL, Larsen HL, Pikkupeura LM, Maciag G, Guiu J, Müller I, Clement DL, Mueller C, Johansen JV, Helin K,
et al
.
610154
; RRID: AB_397555), and anti-DCLK1 (
1:400
dilution; polyclonal rabbit antibody;
Abcam
, catalog no.
ab31704
; RRID: AB_873537). Each well was washed three times for 5 min with PBS, before
incubation
for 1 to 2
(8)
Combined multidimensional single-cell protein and RNA profiling dissects the cellular and functional heterogeneity of thymic epithelial cells
Nature communications
July 10, 2023
Klein F, Veiga-Villauriz C, Börsch A, Maio S, Palmer S, Dhalla F, Handel AE, Zuklys S, Calvo-Asensio I, Musette L,
et al
.
BD Biosciences
), CD146-APC (
1:800
, ME-9F1, Biolegend), EpCMA1-PerCPCy5.5 (
1:800
, G8.8, Biolegend), Dclk1 (
1:1000
,
ab31704
,
Abcam
), HVEM-APC (
1:400
, HMHV-1B18, Biolegend), Ly51-PECy7 (
1:400
, 6C3, Biolegend), MHCII-APC/Fire750
(9)
Targeting DCLK1 attenuates tumor stemness and evokes antitumor immunity in triple-negative breast cancer by inhibiting IL-6/STAT3 signaling
Breast cancer research : BCR
April 17, 2023
Liu H, Yan R, Xiao Z, Huang X, Yao J, Liu J, An G, Ge Y.
USA) using chemiluminescence kit (Millipore, USA). The primary antibodies used above were: anti-DCLK1(
ab31704
,
Abcam
), anti-
SOX2
(
3579T
,
CST
), anti-
NANOG
(
4903T
,
CST
), anti-
OCT4
(
11263-1-AP
,
Proteintech
), anti-
CD44
(10)
Embryonic keratin19+ progenitors generate multiple functionally distinct progeny to maintain epithelial diversity in the adult thymus medulla
Nature Communications
April 12, 2023
Beth Lucas, Andrea J. White, Fabian Klein, Clara Veiga-Villauriz, Adam Handel, Andrea Bacon, Emilie J. Cosway, Kieran D. James, Sonia M. Parnell, Izumi Ohigashi,
et al.
2312434), K19 (EP1580Y,
Abcam
, Cat no:
ab52625
, Lot no: GR3384962-1), DCLK1 (DCAMKL1,
Abcam
, Cat No:
ab31704
, Lot no: GR3357375-3),
CCL21
(
LifeSpan
Technologies, Cat no:
LS-C104634
, Lot no: 55059), SSEA-1-BV421
(11)
CDK8 and CDK19 act redundantly to control the CFTR pathway in the intestinal epithelium
EMBO reports
February 6, 2023
Susana Prieto, Geronimo Dubra, Alain Camasses, Ana Bella Aznar, Christina Begon‐Pescia, Elisabeth Simboeck, Nelly Pirot, François Gerbe, Lucie Angevin, Philippe Jay,
et al.
C,Polyclonal,gift,Rabbit,
PCNA
,Monoclonal,
Santa Cruz
,
Mouse
,
sc‐56
Lysozyme,Polyclonal,Dako,Rabbit,A0099 Anti‐Dclk1,Polyclonal,
Abcam
,Rabbit,
Ab31704
Olfm4,Monoclonal,
Cell signaling
,Rabbit,
39141
β‐Catenin,Monoclonal,BD BioSciences,
Mouse
,"610,154" BrdU,Monoclonal,DSHB,
Mouse
,G3G4 RNA
(12)
Loss of SLC26A3 Results in Colonic Mucosal Immune Dysregulation via Epithelial-Immune Cell Crosstalk
Cellular and molecular gastroenterology and hepatology
January 1, 2023
Jayawardena D, Priyamvada S, Kageyama T, White Z, Kumar A, Griggs TF, Majumder A, Akram R, Anbazhagan AN, Sano T,
et al
.
"
ROR gamma (t) Monoclonal Antibody (B2D), PE, eBioscience
"
Novus
,
NBP1-75516
,
IL-33
Invitrogen
,
PA5-47007
,
IL-33
Millipore
,
05-1567
,P-120 Catenin
Abcam
,
ab31704
,DCLK1 Research Resource Center UIC and Pocono Rabbit Farm,Research Resource Center UIC and Pocono Rabbit
(13)
Defining the early stages of intestinal colonisation by whipworms
Nature Communications
December 1, 2022
María A. Duque-Correa et al.
α-Ki-67 (
1:250
,
Abcam
,
ab16667
), α-chromogranin A (
1:50
,
Abcam
,
ab15160
), α-Dcamlk-1 (
1:200
,
Abcam
,
ab31704
), α-zona occludens-1 (
ZO-1
) protein (
1:200
,
Invitrogen
,
61-7300
) and the lectins
Ulex europaeus
agglutinin
(14)
Sirt6-mediated epigenetic modification of DNA accessibility is essential for Pou2f3-induced thymic tuft cell development
Communications Biology
December 1, 2022
Qian Zhang, Jiayu Zhang, Tong Lei, Zhanfeng Liang, Xue Dong, Liguang Sun, Yong Zhao
104732
,
1:300
dilution), L1CAM-PE (R&D,
clone
555, FAB5674P,
1:20
dilution), rabbit anti-DCLK1 (
Abcam
,
ab31704
,
1:15000
dilution), TCR-β-PE-Cy7 (
Biolegend
,
clone
H57-597,
109222
,
1:500
dilution),
mouse
CD1d loaded
(15)
Thrombin induces IL-8/CXCL8 expression by DCLK1-dependent RhoA and YAP activation in human lung epithelial cells
Journal of biomedical science
November 11, 2022
Yuliani FS, Chen JY, Cheng WH, Wen HC, Chen BC, Lin CH.
(Woburn, MA, USA) and
Invitrogen
(Waltham, MA, USA), respectively. The specific antibodies for DCLK1 (
ab31704
), YAP1 (
ab52771
), YAP phospho Ser127 (
ab76252
), thrombin (
ab92621
), and
E-cadherin
(
ab40772
) were procured
(16)
Microbial regulation of intestinal motility provides resistance against helminth infection
Mucosal Immunology
November 1, 2022
Mati Moyat, Luc Lebon, Olaf Perdijk, Lakshanie C. Wickramasinghe, Mario M. Zaiss, Ilaria Mosconi, Beatrice Volpe, Nadine Guenat, Kathleen Shah, Gillian Coakley,
et al.
mucosal integrity and no
artefacts
. Tuft cells
staining
was performed using anti-DCLK1 antibody (
Abcam
,
AB31704
) and anti-rabbit Alexa Fluor 488 (
Jackson ImmunoResearch
,
111-545-003
). Nuclei were then fluorescently
(17)
The proprotein convertase furin regulates the development of thymic epithelial cells to ensure central immune tolerance
iScience
October 21, 2022
Liang Z, Zhang Z, Zhang Q, Dong X, Yang X, Zhang J, Lei T, Creemers JWM, Zhang B, Zhao Y.
anti-K8 (DSHB, Troma-I),
chicken
anti-K5 (
Biolegend
,
905904
), and rabbit anti-DCAMKL1 (DCLK1) (
Abcam
,
ab31704
). Isolation of thymic
stromal cells
Mouse
thymi were isolated, cleaned of fat, and cut into pieces
(18)
Contribution of Trp63(CreERT2)-labeled cells to alveolar regeneration is independent of tuft cells
eLife
September 21, 2022
Huang H, Fang Y, Jiang M, Zhang Y, Biermann J, Melms JC, Danielsson JA, Yang Y, Qiang L, Liu J,
et al
.
PMID:20023653",MGI: 4436847,"Jackson Laboratories (#007914, R26Ai14)" Antibody,Anti-Dclk1(Rabbit polyclonal),
Abcam
,
ab31704
,
1:200
Antibody,
Anti-CC10 (E-11
)(
Mouse
monoclonal),
Santa Cruz
,
sc-365992
,
1:200
;
Scgb1a1
Antibody,Anti-
SCGB1A1
(
Rat
(19)
Live-cell imaging in human colonic monolayers reveals ERK waves limit the stem cell compartment to maintain epithelial homeostasis
eLife
September 12, 2022
Kelvin W Pond, Julia M Morris, Olga Alkhimenok, Reeba P Varghese, Carly R Cabel, Nathan A Ellis, Jayati Chakrabarti, Yana Zavros, Juanita L Merchant, Curtis A Thorne,
et al.
(
Thermofisher
,
D21490
)
Rat
monoclonal anti-
Ki67
(
Thermofisher
,
14-5698-80
)Rabbit polyclonal DCAMKL (
Abcam
,
ab31704
)Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (
Thermofisher
,
A-11006
)
Donkey anti-Goat IgG (H+L) Secondary Antibody [DyLight 488] (Pre-adsorbed
(20)
Injury-induced pulmonary tuft cells are heterogenous, arise independent of key Type 2 cytokines, and are dispensable for dysplastic repair
eLife
September 8, 2022
Barr J, Gentile ME, Lee S, Kotas ME, Fernanda de Mello Costa M, Holcomb NP, Jaquish A, Palashikar G, Soewignjo M, McDaniel M,
et al
.
Fiji softwares. Primary antibodies used: rabbit anti-
Dclk1
(
1:500
dilution; catalog no.
ab37994
or
ab31704
,
Abcam
),
chicken
anti-
Krt5
(
1:1000
dilution; catalog no.
905901
,
BioLegend
)
sheep
anti-eGFP
(
1:500
,
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Anti-DCAMKL1 antibody from Abcam
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
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