Fig 1: Chkb regulates the gene expression of the members of the Ppar family as well as Ppar target genes.A Relative gene expression of the Ppar family members. B Western blot of hindlimb (quadriceps) samples from three distinct (lanes 1–3) Chkb+/+, four distinct (lanes 4–7) Chkb+/- and three distinct (lanes 8–10) Chkb-/- mice probed with anti-Para, anti- Pparb, anti-Pparg, anti-Cpt1b, and anti-Gapdh antibodies. Bottom: densitometry of the western blot data shows the ratio of Ppara, Pparb, Pparg, and Cpt1b to Gapdh. Values are means ± SD. For A, n = 3 (Chkb+/+), n = 3 (Chkb+/-) and n = 3 (Chkb-/-) mice per group. For B, n = 3 (Chkb+/+), n = 4 (Chkb+/-) and n = 3 (Chkb-/-) mice per group. For A one-way ANOVA with Tukey’s multiple comparison test, p < 0.0001 (Ppara), p < 0.0001 (Pparb), p = 0.0087 (Pparg). *p < 0.05, **p < 0.01. For (B) one-way ANOVA with Tukey’s multiple comparison test, p = 0.0166 (Ppara), p = 0.0014 (Pparb), p = 0.0010 (Pparg), p = 0.00018 (Cpt1b). *P < 0.05, **p < 0.01. C Fold-Change (2^ (- Delta Delta CT)) is the normalized gene expression (2^(- Delta CT)) in the Chkb deficient hindlimb sample divided the normalized gene expression (2^ (- Delta CT)) in the control sample. Fold-change values greater than one indicates a positive- or an up-regulation. Fold-change values less than one indicate a negative or down-regulation, and the fold-regulation is the negative inverse of the fold-change. The p values are calculated based on a two-sided student’s t-test of the replicate 2^ (- Delta CT) values for each gene in the Chkb+/+ group and Chkb-/- groups. *p < 0.05, **p < 0.01. n = 3 independent samples per group. D The clustergram of the Ppar family, Rxr family and Ppar coactivators across three genotypes. E Fold-Change (2^ (- Delta Delta CT)) is the normalized gene expression (2^(- Delta CT)) in the Chkb deficient hindlimb sample divided the normalized gene expression (2^ (- Delta CT)) in the control sample. Fold-change values greater than one indicates a positive- or an up-regulation. Fold-change values less than one indicate a negative or down-regulation, and the fold-regulation is the negative inverse of the fold-change. The p values are calculated based on two-sided student’s t-test of the replicate 2^ (- Delta CT) values for each gene in the Chkb+/+ group and Chkb-/- groups. *p < 0.05, **p < 0.01. n = 3 independent samples per group. F The clustergram of the Ppar family, Rxr family and Ppar coactivators across three genotypes. Average arithmetic means of the expression of 4 housekeeping genes (Actb, B2m, Gusb and Hsp90ab1) were used to normalize the expression of all the studied genes. Source data are provided as a Source Data file.