Anti-Detyrosinated alpha Tubulin antibody - Microtubule Marker ab48389 from Abcam

Product Specs
ItemAnti-Detyrosinated alpha Tubulin antibody - Microtubule Marker
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab48389
Antibody TypePrimary
IsotypeIgG
Quantity100 UG
ApplicationsWestern blot
ClonalityPolyclonal
HostRabbit
Conjugate/TagUnconjugated
Gene NameTUBA1A
ReactivityHuman
TargetDetyrosinated alpha Tubulin

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Microtubule-mediated GLUT4 trafficking is disrupted in insulin-resistant skeletal muscleeLifeApril 19, 2023Jonas R Knudsen, Kaspar W Persson, Carlos Henriquez-Olguin, Zhencheng Li, Nicolas Di Leo, Sofie A Hesselager, Steffen H Raun, Janne R Hingst, Raphaël Trouillon, Martin Wohlwend, et al.following antibodies were used, raised in rabbit: GLUT4 (PA5-23052, Invitrogen), detyrosinated α-tubulin (AB48389, Abcam), Syntaxin6 (110 062, Synaptic Systems), or in mouse: GLUT4 (MAB8654, R&D Systems), α-tubulin

(2) Microtubule-associated proteins MAP7 and MAP7D1 promote DNA double-strand break repair in the G1 cell cycle phaseiScienceMarch 17, 2023Dullovi A, Ozgencil M, Rajvee V, Tse WY, Cutillas PR, Martin SA, Hořejší Z.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, anti-phospho BRCA1 (S1336),GenScript,N/A detyrosinated α-tubulin,Abcam,Cat# ab48389 RRID:AB_869990 Phospho-CHK2 pT68,Cell Signaling,Cat# 2661 RRID:AB_331479 Flag (anti-DYKDDDK),Cell Signaling

(3) α-Tubulin detyrosination links the suppression of MCAK activity with taxol cytotoxicityJournal of Cell BiologyFebruary 6, 2023Danilo Lopes, Alexandre L. Seabra, Bernardo Orr, Helder Maiatowere used to incubate membranes overnight at 4°C: rabbit anti-detyrosinated α-tubulin (Polyclonal; ab48389; 1:2,000; Abcam; the antibody used in the NCI-60 screen), rabbit anti-detyrosinated α-tubulin (1:10,000;

(4) Elevation of Cytoplasmic Calcium Suppresses Microtentacle Formation and Function in Breast Tumor CellsCancers (Basel)January 31, 2023Katarina T. Chang, Keyata N. Thompson, Stephen J. P. Pratt, Julia A. Ju, Rachel M. Lee, Trevor J. Mathias, Makenzy L. Mull, David A. Annis, Eleanor C. Ory, Megan B. Stemberger, et al.Danvers, MA, USA), Anti-α-tubulin (1:1000, Sigma T6199), Anti-Detyrosinated alpha tubulin (1:1000, Abcam, ab48389, Cambridge, UK), Anti-GAPDH (1:1000, Santa Cruz sc32233, Santa Cruz, CA, USA), Anti-Myosin Light Chain

(5) JIP3 links lysosome transport to regulation of multiple components of the axonal cytoskeletonCommunications BiologyDecember 1, 2022N. M. Rafiq, L. L. Lyons, S. Gowrishankar, P. De Camilli, S. M. Ferguson(Cell Signaling Technology, catalog no. 5335, dilution 1:250); anti-detyrosinated α-tubulin (Abcam, catalog no. ab48389, dilution 1:250); anti-tyrosinated α-tubulin (Millipore Sigma, clone YL1/2, dilution 1:250). Cells were

(6) Desmin intermediate filaments and tubulin detyrosination stabilize growing microtubules in the cardiomyocyteBasic Res CardiolNovember 3, 2022Alexander K. Salomon, Sai Aung Phyo, Naima Okami, Julie Heffler, Patrick Robison, Alexey I. Bogush, Benjamin L. Prossermonoclonal (Sigma T6793-100UL); western blot: 1:1000. Detyrosinated tubulin; rabbit polyclonal (Abcam ab48389); western blot: 1:1000. Alpha tubulin; mouse monoclonal, clone DM1A (Cell Signaling #3873); western blot

(7) Tension of plus-end tracking protein Clip170 confers directionality and aggressiveness during breast cancer migrationCell death & diseaseOctober 8, 2022Hu Y, Xie Q, Wu X, Liu W, Li D, Li C, Zhao W, Chen L, Zheng Z, Li G, et al.western blotting 1:2000) were purchased from Boster (Shanghai, China). Anti-detyrosinated alpha-tubulin (ab48389, western blotting 1:500) was obtained from Abcam (Cambridge, UK). Rhodamine [tetramethylrhodamine (TRITC)]-conjugated

(8) Human alveolar progenitors generate dual lineage bronchioalveolar organoidsCommunications biologyAugust 25, 2022Hoffmann K, Obermayer B, Hönzke K, Fatykhova D, Demir Z, Löwa A, Alves LGT, Wyler E, Lopez-Rodriguez E, Mieth M, et al.incubated overnight, 4 °C: acetylated Tubulin (1:100, Sigma, T7451), detyrosinated Tubulin (1:100, Abcam, ab48389), E-Cadherin (1:100, BD Biosciences, 610181), HTII-280 (1:200, Terrace, TB-27AHT2-280), FOXM1 (1:100,

(9) Mathematical modeling of the microtubule detyrosination/tyrosination cycle for cell-based drug screening designPLOS Computational BiologyJune 27, 2022Jeremy Grignard, Véronique Lamamy, Eva Vermersch, Philippe Delagrange, Jean-Philippe Stephan, Thierry Dorval, François Fagesanti-tyrosinated tubulin antibody (Abcam, Ab6160 1:1000) and anti-detyrosinated tubulin antibody (Abcam Ab48389 1:200) in a 0.5% triton solution. A single primary antibody has been used both for tyrosinated and detyrosinated

(10) RNA localization in confined cells depends on cellular mechanical activity and contributes to confined migrationiScienceFebruary 18, 2022Moriarty RA, Mili S, Stroka KM.detyrosinated tubulin,Rabbit polyclonal detyrosinated tubulin "(1:250 IF, 1:1000 WB)",Abcam,Cat#: ab48389; RRID:AB_869990 "Mouse monoclonal α-tubulin, clone DM1A","Mouse monoclonal α-tubulin, clone DM1A","Mouse

(11) Transcriptional, Post-Transcriptional, and Post-Translational Mechanisms Rewrite the Tubulin Code During Cardiac Hypertrophy and FailureFrontiers in cell and developmental biologyJanuary 1, 2022Phyo SA, Uchida K, Chen CY, Caporizzo MA, Bedi K, Griffin J, Margulies K, Prosser BL.β-tubulin,Abcam,Rabbit,Poly,ab6046,1:1500 Acetylated α-tubulin,Abcam,Mouse,Mono,ab24610,1:1000 Detyrosinated α-tubulin,Abcam,Rabbit,Poly,ab48389,1:1000 Polyglutamylated α-tubulin,Adipogen,Mouse,Mono,50-436-394,1:500 Δ2 α-tubulin,Moutin Lab,Rabbit,Poly,,1:5000 Polyglycylated

(12) Overexpression of Lin28A in neural progenitor cells in vivo does not lead to brain tumor formation but results in reduced spine densityActa Neuropathologica CommunicationsDecember 1, 2021Maximilian Middelkamp et al.rabbit Gli2 (Abcam, ab167389, 1:1000), rabbit APC (Abcam, ab15270, 1:1000), rabbit α-tubulin (Abcam, ab48389, 1:10,000), mouse HSC70 (Santa Cruz, sc-7298, 1:6000); blocking and buffer 5% BSA or milk in TBST. Secondary

(13) Tubulin acetylation increases cytoskeletal stiffness to regulate mechanotransduction in striated muscleThe Journal of general physiologyJuly 5, 2021Coleman AK, Joca HC, Shi G, Lederer WJ, Ward CW.Thermo Fisher Scientific), acetylated (T7451, clone 6-11B-1; Sigma-Aldrich), or detyrosinated tubulin (ab48389; Abcam). Blots were then labeled with the appropriate corresponding secondary antibody and detected

(14) MARK4 controls ischaemic heart failure through microtubule detyrosinationNatureJune 1, 2021Yu X, Chen X, Amrute-Nayak M, Allgeyer E, Zhao A, Chenoweth H, Clement M, Harrison J, Doreth C, Sirinakis G, et al.with primary antibodies overnight at 4°C. The primary antibodies were: Detyrosinated α-tubulin (Abcam, ab48389, used at 1:200), α-tubulin (CST, 3873S, used at 1:200), MARK4 (Abcam, ab124267, used at 1:200), APC anti-mouse CD45

(15) Cochlear supporting cells require GAS2 for cytoskeletal architecture and hearingDevelopmental CellMay 7, 2021Tingfang Chen et al.anti-MYO7A (1:350),Proteus Biosc.,Cat#25–6790 Rabbit anti Detyrosinated-tubulin (1:200),Abcam,Cat#ab48389 Mouse anti-Alpha-tubulin (1:400),Cell Signaling,3873 Mouse anti-Acetylated-tubulin (1:400),Sigma,Cat#T6793 Rabbit

(16) Knockout of vasohibin‐2 reduces tubulin carboxypeptidase activity and increases paclitaxel sensitivity in ovarian cancerCancer MedicineApril 1, 2021Takahiro Koyanagi et al.Enhance Solution 1 (Toyobo Life Science): anti‐detyrosinated alpha tubulin rabbit polyclonal (cat. no. ab48389; Abcam, Cambridge, UK), anti‐alpha tubulin mouse monoclonal (cat. no. sc‐32293; Santa Cruz Biotechnology, Inc

(17) Altered cytoskeletal arrangement in induced pluripotent stem cells (iPSCs) and motor neurons from patients with riboflavin transporter deficiencyDisease models & mechanismsJanuary 19, 2021Niceforo A, Marioli C, Colasuonno F, Petrini S, Massey K, Tartaglia M, Bertini E, Moreno S, Compagnucci C.Merck KGaA, Germany), 1:500 for 2 h at room temperature; anti-α-tubulin detyrosinated antibody (Detyr-TUB, AB48389, Lot GR19854, Abcam), 1:200 overnight at 4°C; anti-hRFVT1 polyclonal antibody (GTX87668, Lot 821401795,

(18) Cross-resistance of cisplatin selected cells to anti-microtubule agents: Role of general survival mechanismsTranslational oncologyJanuary 1, 2021Patel RP, Kuhn S, Yin D, Hotz JM, Maher FA, Robey RW, Gottesman MM, Horibata S.(1:1000; Cell Signaling; Cat# 3033), anti-detyrosinated alpha tubulin antibody (1:1000; Abcam; Cat# ab48389), anti-alpha tubulin antibody (DM1A) (1:5000; Abcam, Cat# 7291), and anti-GAPDH (1:1000; American Research Products, Inc

(19) Affinity Purification of Label-free Tubulins from Xenopus Egg ExtractsSTAR ProtocolsDecember 30, 2020Sebastian Reusch, Abin Biswas, William Graham Hirst, Simone ReberB-5-1-2),Sigma-Aldrich,Cat #: T5168; RRID: AB_477579 Tubulin C terminus with tyrosine removed,Abcam,Cat #: ab48389; RRID: AB_869990 Tyrosinated C terminus of tubulin,Abcam,Cat #: ab6160; RRID: AB_305328 Acetylated

(20) Cep55 overexpression promotes genomic instability and tumorigenesis in miceCommunications biologyOctober 21, 2020Sinha D, Nag P, Nanayakkara D, Duijf PHG, Burgess A, Raninga P, Smits VAJ, Bain AL, Subramanian G, Wall M, et al.α-Tubulin (T9026), γ-Tubulin (T5192), Acetylated tubulin (T7451; Sigma) and detyrosinated tubulin (ab48389; Abcam). DNA combing assay The DNA fiber protocol was followed as described previously by us and others46,47.

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Anti-Detyrosinated alpha Tubulin antibody - Microtubule Marker from Abcam

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Anti-Detyrosinated alpha Tubulin antibody - Microtubule Marker from Abcam

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