Anti-CD45 antibody [B-A11] (FITC) ab27287 from Abcam

Product Specs
ItemAnti-CD45 antibody [B-A11] (FITC)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab27287
Antibody TypePrimary
ClonalityMonoclonal
CloneB-A11
IsotypeIgG1
ApplicationsFlow Cytometry
Quantity50 test
Conjugate/TagFITC
HostMouse, Nude mouse
Research AreaImmuno-oncology
Gene NamePTPRC
ReactivityHuman
TargetCD45

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) BRD4 facilitates osteogenic differentiation of human bone marrow mesenchymal stem cells through WNT4/NF-κB pathwayJournal of Orthopaedic Surgery and ResearchNovember 18, 2023Tao Ning, Huihui Guo, Mingming Ma, Zhengang Zhaanti-CD34 (ab187284, Abcam), anti-CD44 (ab23396, Abcam), anti-CD90 (ab25322, Abcam), and anti-CD45 (ab27287, Abcam). Data were analyzed by FACSCalibur flow cytometer. Quantitative real-time PCR (qRT-PCR) Total

(2) Role of FGF10/FGFR2b Signaling in Homeostasis and Regeneration of Adult Lacrimal Gland and Corneal Epithelium ProliferationInvestigative Opthalmology & Visual ScienceJanuary 30, 2023Emma N. Finburgh, Olivier Mauduit, Takako Noguchi, Jennifer J. Bu, Anser A. Abbas, Dominic F. Hakim, Saverio Bellusci, Robyn Meech, Helen P. Makarenkova, Natalie A. Afsharifluorescein isothiocyanate (FITC)-conjugated CD45 mouse monoclonal antibody (clone B-A11, RRID:AB_868944, #ab27287; Abcam, Cambridge, UK) and allophycocyanin (APC)-conjugated CD19 rat monoclonal antibody (Clone eBio1D3

(3) miR-31 from Mesenchymal Stem Cell-Derived Extracellular Vesicles Alleviates Intervertebral Disc Degeneration by Inhibiting NFAT5 and Upregulating the Wnt/Î²-Catenin PathwayStem Cells InternationalOctober 20, 2022Baodong Wang, Na Xu, Li Cao, Xiaojun Yu, Shanxi Wang, Qikun Liu, Yinguang Wang, Haoran Xu, Yang Cao(1 : 100, ab11414, Abcam), CD73 (1 : 50, ab239246, Abcam), CD90 (1 : 1000, ab23894, Abcam), CD45 (1 : 50, ab27287, Abcam), CD34 (1 : 50, ab131589, Abcam), CD14 (1 : 200, ab28061, Abcam), CD19 (1 : 50, ab134114, Abcam),

(4) Chondrogenic primed extracellular vesicles activate miR-455/SOX11/FOXO axis for cartilage regeneration and osteoarthritis treatmentnpj Regenerative MedicineSeptember 16, 2022Ye Sun, Jie Zhao, Qiang Wu, Yuxin Zhang, Yongqing You, Wenbo Jiang, Kerong Daiincubated with FITC-labeled antibodies against CD29, CD34, CD45, CD90, and CD105 (ab150002, ab78165, ab27287, ab124527, and ab11415, Abcam dilution at 1:2000) at 4 °C in the dark for 30 min. Cells were washed

(5) Leukemia Inhibitory Factor Facilitates Self-Renewal and Differentiation and Attenuates Oxidative Stress of BMSCs by Activating PI3K/AKT SignalingOxidative Medicine and Cellular LongevitySeptember 5, 2022Youde Liang et al.CD44-PE (ab23396), CD90-APC (ab25322), CD105-PE/Cy7 (ab272352), CD146-FITC (ab78451), and CD45-FITC (ab27287) on the ice in the dark for 30 min. After centrifuging at 1000 rpm for 5 min, the supernatant was discarded.

(6) Differentiation of Alginate-Encapsulated Wharton Jelly-Derived Mesenchymal Stem Cells into Insulin Producing CellsCell JAugust 28, 2022Zahra Poursafavi, Saeid Abroun, Saeid Kaviani, Nasim Hayati Hayati Roodbarioptimal dilution of conjugated antibodies that included anti-CD73-FITC (ab28061), anti-CD45- FITC (ab27287), anti-CD90-FITC (ab11155), antiCD34-PE (ab157304), and anti-CD105-PE (ab91138), all from Abcam (Cambridge,

(7) Bone marrow mesenchymal stem cell-derived extracellular vesicles containing miR-181d protect rats against renal fibrosis by inhibiting KLF6 and the NF-κB signaling pathwayCell death & diseaseJune 7, 2022Wang SJ, Qiu ZZ, Chen FW, Mao AL, Bai JC, Hong YJ, Zhang ZP, Zhu WA, Zhang ZW, Zhou H.(ab21845), CD44 (ab25024), and CD73 (ab239246) while hematopoietic markers, such as CD34 (ab18224), CD45 (ab27287), and HLA-DR (ab1182) were absent. All antibodies were purchased from Abcam Inc. (Cambridge, MA). Isolation

(8) Lysophosphatidylcholine Offsets the Protective Effects of Bone Marrow Mesenchymal Stem Cells on Inflammatory Response and Oxidative Stress Injury of Retinal Endothelial Cells via TLR4/NF-κB SignalingJournal of Immunology ResearchOctober 14, 2021Haijun Zhao, Yanhui Hetwice with 1x PBS to count the cells. Human BMSCs (1 × 106 cells/ml) were probed with CD45 antibody (ab27287, Abcam, Cambridge, MA, USA), CD105 antibody (ab155367, Abcam), and CD73 antibody (ab157335, Abcam) and

(9) Isolation and identification of exosomes from feline plasma, urine and adipose-derived mesenchymal stem cellsBMC veterinary researchAugust 12, 2021Li D, Luo H, Ruan H, Chen Z, Chen S, Wang B, Xie Y.anti-CD34-PE (cat.no.ab23830; Abcam), anti-CD44- FITC (cat.no.MA1–10229; Invitrogen), anti-CD45-FITC (cat.no.ab27287; Abcam), anti-CD90-PE (cat.no.11–0900-81; Invitrogen), anti-CD105-FITC (cat.no.ab11415; Abcam),and anti-HLA-DR-FITC

(10) MicroRNA-21 from bone marrow mesenchymal stem cell-derived extracellular vesicles targets TET1 to suppress KLF4 and alleviate rheumatoid arthritisTherapeutic Advances in Chronic DiseaseApril 24, 2021Guo-Qing Li, Yu-Xuan Fang, Ying Liu, Fan-Ru Meng, Xia Wu, Chun-Wang Zhang, Yu Zhang, Yan-Qing Liu, Dan Liuand CD73 (ab239246, Abcam) were positive, while hematopoietic markers CD34 (ab18224, Abcam), CD45 (ab27287, Abcam), and HLA-DR (ab1182, Abcam) were negative. Osteogenic and lipogenic differentiation and staining BMSCs

(11) Manufacturing and banking canine adipose-derived mesenchymal stem cells for veterinary clinical applicationBMC veterinary researchMarch 1, 2021Luo H, Li D, Chen Z, Wang B, Chen S.anti-CD34-PE (cat.no.ab23830; Abcam), anti-CD44- FITC (cat.no.MA1-10229; Invitrogen), antiCD45-FITC (cat.no.ab27287; Abcam), and anti-CD90-PE (cat.no.11-0900-81; Invitrogen) or their respective isotype controls. Cells

(12) Ultrasound‑targeted microbubble destruction‑mediated Galectin‑7‑siRNA promotes the homing of bone marrow mesenchymal stem cells to alleviate acute myocardial infarction in ratsInternational Journal of Molecular MedicineDecember 23, 2020Xin Wei, Yan Zheng, Weilin Zhang, Jing Tan, Hong Zhengno. ab21845; 1:10), CD34 (cat. no. ab78165; 1:10), CD44 (cat. no. ab30405; 1:10) and CD45 (cat. no. ab27287; 1:10) (all purchased from Abcam) were added. The corresponding negative control (NC) was set for each

(13) Human Adipose-Derived Mesenchymal Stem Cells-Derived Exosomal microRNA-19b Promotes the Healing of Skin Wounds Through Modulation of the CCL1/TGF-β Signaling AxisClinical, Cosmetic and Investigational DermatologyDecember 15, 2020Guoxiu Cao, Bei Chen, Xian Zhang, Hongyun Chenantibody for 15 minutes in darkness. The antibodies included CD34 (ab18227), CD44 (ab27285), CD45 (ab27287) and CD105 (ab53318) (all from Abcam, Cambridge, USA) or with appropriate isotype control IgG (Abcam).

(14) Bone Marrow Mesenchymal Stem Cell-Derived Exosomal miR-25 Regulates the Ubiquitination and Degradation of Runx2 by SMURF1 to Promote Fracture Healing in MiceFrontiers in MedicineDecember 7, 2020Yikun Jiang, Jun Zhang, Zhengwei Li, Guoliang Jia(ab239246, Abcam) while negative for hematopoietic markers, including CD34 (ab18224, Abcam), CD45 (ab27287, Abcam), and human leukocyte antigen-DR (HLA-DR) (ab1182, Abcam). Exosome Isolation and Purification In

(15) Role of the HIF‑1α/SDF‑1/CXCR4 signaling axis in accelerated fracture healing after craniocerebral injuryMolecular Medicine ReportsJuly 28, 2020Yonghua Xue, Zhikun Li, Yi Wang, Xiaodong Zhu, Ruixi Hu, Wei Xuab21845; Abcam), anti‑CD44‑FITC (2 µg; cat. no. ab25064; Abcam) or anti‑CD45‑FITC (10 µl; cat. no. ab27287; Abcam), and analyzed using a Coulter Epics XL‑MCL flow cytometer (BD Biosciences) with EXPO32 software

(16) Activation of SIRT1 promotes cartilage differentiation and reduces apoptosis of nucleus pulposus mesenchymal stem cells via the MCP1/CCR2 axis in subjects with intervertebral disc degenerationInternational Journal of Molecular MedicineJuly 3, 2020Xuancheng Ou, Jinwei Ying, Xuedong Bai, Chaofeng Wang, Dike Ruan(FITC)-conjugated primary antibodies (all from Abcam) against CD34 (1:50; cat. no. ab78165), CD45 (1:50; cat. no. ab27287), CD73 (1:50; cat. no. ab54217) and CD90 (1:50; cat. no. ab124527) at 4°C for 45 min in the dark. An

(17) MSC-Secreted Exosomal H19 Promotes Trophoblast Cell Invasion and Migration by Downregulating let-7b and Upregulating FOXO1Molecular therapy. Nucleic acidsMarch 6, 2020Chen Y, Ding H, Wei M, Zha W, Guan S, Liu N, Li Y, Tan Y, Wang Y, Wu F.antibody, fluorescein isothiocyanate-conjugated antibodies against CD19 (ab24936), CD34 (ab18227), CD45 (ab27287), CD73 (ab239246), CD90 (ab226), CD105 (ab53318), polyethylene glycol/Cy5-conjugated antibody against

(18) Free-Floating Mesothelial Cells in Pleural Fluid After Lung SurgeryFrontiers in MedicineApril 5, 2018Arne Kienzle, Andrew B. Servais, Alexandra B. Ysasi, Barry C. Gibney, Cristian D. Valenzuela, Willi L. Wagner, Maximilian Ackermann, Steven J. Mentzerobtained from commercial sources. Staining for CD45 [FITC conjugated mouse monoclonal (B-A11), Abcam, ab27287] and CD71 [AlexaFluor647 conjugated mouse monoclonal (MEM75), Abcam, ab187777] was performed on ice

(19) Differentiation of human umbilical cord Wharton's jelly-derived mesenchymal stem cells into endometrial cellsStem cell research & therapyNovember 2, 2017Shi Q, Gao J, Jiang Y, Sun B, Lu W, Su M, Xu Y, Yang X, Zhang Y.with 5% CO2. The antibodies used to identify WJ-MSCs were anti-CD14-FITC (ab28061), anti-CD45-FITC (ab27287), anti-CD79a-PE (ab155344), anti-CD90-FITC (ab11155), anti-CD34-PE (ab157304), anti-CD105-PE (ab91138),

(20) Effect of Bone Marrow Aspirate Concentrate-Platelet-Rich Plasma on Tendon-Derived Stem Cells and Rotator Cuff Tendon TearCell transplantationMay 9, 2017Kim SJ, Song DH, Park JW, Park S, Kim SJ.with fluorescein isothiocyanate (FITC)-conjugated mouse-specific antibodies to human CD45 (Catalog No. ab27287; Abcam) and CD105 (Catalog No. ab18278; Abcam) for 1 h at room temperature. Nonspecific FITC or PE-conjugated IgG

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Anti-CD45 antibody [B-A11] (FITC) from Abcam

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Anti-CD45 antibody [B-A11] (FITC) from Abcam

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