Anti-beta III Tubulin antibody [EP1569Y] ab52623 from Abcam

Product Specs
ItemAnti-beta III Tubulin antibody [EP1569Y]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab52623
Antibody TypePrimary
ClonalityMonoclonal
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Multiplex immunohistochemistry, Western blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Multiplex immunohistochemistry, Western blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Multiplex immunohistochemistry, Western blot
HostRabbit
CloneEP1569Y
Quantity10 UL, 40 UL, 100 UL
IsotypeIgG
Conjugate/TagUnconjugated
Research AreaNeuroscience
Gene NameTUBB3
ValidationKnockOut Validated
ReactivityHuman, Mouse, Rat
Targetbeta III Tubulin

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Phosphorylated S6K1 and 4E-BP1 play different roles in constitutively active Rheb-mediated retinal ganglion cell survival and axon regeneration after optic nerve injuryNeural Regeneration ResearchNovember 1, 2023Jikuan Jiang, Lusi Zhang, Jingling Zou, Jingyuan Liu, Jia Yang, Qian Jiang, Peiyun Duan, Bing Jiang1:400, Abcam, Cambridge, UK, Cat# ab78078, RRID: AB_2256751), β-III tubulin (rabbit, 1:400, Abcam, Cat# ab52623, RRID: AB_869991), hemagglutinin (HA; rabbit, 1:150, Abcam, Cat# ab236632; RRID: AB_2864361), phospho-AKT-T308

(2) Repression of ferroptotic cell death by mitochondrial calcium signalingResearch squareJuly 10, 2023Chen J, Zhao B, Wang S, Ma A, Dong H, Cheng X, Lin S, Li X, Herring LE, Xin G, et al.section on 20μM and immunostaining was performed according to standard procedures using anti-TUJ1 (ab52623, Abcam) and DAPI staining. TUNEL was performed on paraffin sections using the in situ cell death detection

(3) Simultaneous induction of vasculature and neuronal network formation on a chip reveals a dynamic interrelationship between cell typesCell communication and signaling : CCSJune 14, 2023Isosaari L, Vuorenpää H, Yrjänäinen A, Kapucu FE, Kelloniemi M, Pakarinen TK, Miettinen S, Narkilahti S.monoclonal anti-β-tubulin III (βtubIII, chicken, 1:100, ab41489, Abcam), anti-βtubIII (rabbit, 1:250, ab52623, Abcam) anti-microtubule-associated protein 2 (MAP-2, rabbit, 1:200, AB5622, Millipore), anti-MAP-2

(4) Differentiating PC12 cells to evaluate neurite densities through live-cell imagingSTAR protocolsMarch 17, 2023Karliner J, Merry DE.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, "Anti-beta III tubulin antibody (rabbit), 1:1000 for Western blot",Abcam,Cat#ab52623; RRID: AB_869991; Clone#EP1569Y "Anti-beta III tubulin antibody – neuronal marker (mouse), 1:1000 for immunocytochemistry

(5) Low-intensity pulsed ultrasound promotes skeletal muscle regeneration via modulating the inflammatory immune microenvironmentInternational Journal of Biological SciencesFebruary 5, 2023Haocheng Qin, Zhiwen Luo, Yaying Sun, Zhong He, Beijie Qi, Yisheng Chen, Junlong Wang, Ce Li, Weiwei Lin, Zhihua Han, et al.LaminB1[ab16048; Abcam], CD206 [ab64693; Abcam]. 1:5000 dilution for GAPDH [ab8245; Abcam], β-Tubulin [ab52623; Abcam]). The next day, the secondary antibody was incubated at RT for 1h after 3 required washing procedures.

(6) Neural stem cell-derived exosome as a nano-sized carrier for BDNF delivery to a rat model of ischemic strokeNeural regeneration researchFebruary 1, 2023Zhu ZH, Jia F, Ahmed W, Zhang GL, Wang H, Lin CQ, Chen WH, Chen LK.differentiation-related markers rabbit monoclonal anti-class III β-tubulin (Tuj1; 1:500, Abcam, Cat# ab52623, RRID: AB_869991), mouse monoclonal anti-glial fibrillary acidic protein (GFAP; 1:50, Abcam, Cat# ab4648,

(7) Pericytes control vascular stability and auditory spiral ganglion neuron survivaleLifeJanuary 31, 2023Yunpei Zhang, Lingling Neng, Kushal Sharma, Zhiqiang Hou, Anatasiya Johnson, Junha Song, Alain Dabdoub, Xiaorui Shimonoclonal),Abcam,Cat#: ab32362,1:50 Antibody,anti-β-III Tubulin [EP1569Y] (Rabbit monoclonal),Abcam,Cat#: ab52623,1:200 Antibody,anti-CD31 [MEC 7.46] (Rat monoclonal),Abcam,Cat#: ab7388,1:100 Antibody,anti-VEGFR2

(8) Closing the Gap between the Auditory Nerve and Cochlear Implant Electrodes: Which Neurotrophin Cocktail Performs Best for Axonal Outgrowth and Is Electrical Stimulation Beneficial?International journal of molecular sciencesJanuary 19, 2023Schmidbauer D, Fink S, Rousset F, Löwenheim H, Senn P, Glueckert R.Antigen,Host,Company,Product,Dilution Beta-III-tubulin/Tuj1,Rabbit,Abcam,ab52623,1:1000 Rabbit IgG + Alexa 546 *,Donkey,Thermo Fisher Scientific,A10040,1:1500 trkB,Goat,R&D Systems,AF1494,1:2000 trkC,Goat,R&D Systems

(9) Hyperglycemic Neurovasculature‐On‐A‐Chip to Study the Effect of SIRT1‐Targeted Therapy for the Type 3 Diabetes “Alzheimer's Disease”Advanced ScienceDecember 1, 2022Minjeong Jang, Nakwon Choi, Hong Nam Kim(PHC3015, Gibco, United States) on 2D plates and NV chip for 48 h. Antibodies and Chemicals Anti‐Tuj1 (ab52623, Abcam, United Kingdom), anti‐CD31 (PECAM‐1, sc‐376764, Santa Cruz, United States), anti‐VE‐cadherin

(10) A synthetic pregnenolone analog promotes microtubule dynamics and neural developmentCell & bioscienceDecember 1, 2022Kolas V, Bandonil JSA, Wali N, Hsia KC, Shie JJ, Chung BC.tubulin, clone 6-11B-1",Sigma,Cat#T6793,1:500 Rabbit monoclonal anti-III-beta tubulin [EP1569Y],Abcam,Cat#ab52623,1:500 Rabbit polyclonal anti-beta tubulin,Proteintech,10094-1-AP,1:200 "Goat anti-mouse IgG (H+L)

(11) Extracellular vesicles derived from astrocyte-treated with haFGF_14-154 attenuate Alzheimer phenotype in AD miceTheranosticsMay 9, 2022Dong Peng et al.monoclonal anti-β-actin (1:5000, Bioworld, BS6007M), and mouse monoclonal anti-β-III-tubulin (1:5000, Abcam, ab52623) antibodies. The membranes were incubated with the secondary antibody (peroxidase-conjugated affinity-purified

(12) miR-99 family is potential target to reverse cerium dioxide nanoparticle-induced placental cell dysfunctionAnnals of Translational MedicineApril 1, 2022Mengmeng Yao, Xiaoli Ji, Yuqing Zhang, Zhilei Mao, Xia Chifixed with 4% paraformaldehyde for 30 min. Microtubules was revealed by anti-β-tubule antibody (Abcam, ab52623), combined with FITC-conjugated secondary goat antibody (Beyotime, A0562, A0568). Microfilaments were

(13) Circular RNA drives resistance to anti-PD-1 immunotherapy by regulating the miR-30a-5p/SOX4 axis in non-small cell lung cancerCancer Drug ResistanceMarch 25, 2022Jing Wu, Meng-Xuan Zhu, Ke-Sang Li, Ling Peng, Peng-Fei ZhangUniversity. And the antibody used in western blot and circRIP assays were listed as follows: Tubulin (Abcam, ab52623), AGO2 (Abcam, ab186733), and SOX4 (Abcam, ab70598). Transfection of lentiviral vectors, plasmids,

(14) Lacrimal Gland Microenvironment Changes After Obstruction of Lacrimal Gland DuctsInvestigative Opthalmology & Visual ScienceMarch 15, 2022Xin He, Shaopan Wang, Huimin Sun, Hui He, Yalin Shi, Yiming Wu, Han Wu, Zuguo Liu, Jingyi Zhuang, Wei Li1:200, ab184675), anti-p63 (1:200, ab124762), anti-Ki67 (1:400, ab16667), anti-β-III tubulin (1:200, ab52623) and anti-vimentin (1:200, ab8978) antibodies were from Abcam (Cambridge, UK). Anti-laminin 5 antibody

(15) NADPH Oxidase 3 Deficiency Protects From Noise-Induced Sensorineural Hearing LossFrontiers in Cell and Developmental BiologyFebruary 22, 2022Francis Rousset, German Nacher-Soler, Vivianne Beatrix Christina Kokje, Stéphanie Sgroi, Marta Coelho, Karl-Heinz Krause, Pascal Senn(30 s) was employed as counterstaining agent, whereas DAPI and β-III tubulin (1/1000, rabbit, Abcam, ab52623) immunostaining were used for Fluorescent multiplex assay. Finally, the slides were dehydrated, cleared

(16) Histone deacetylase HDAC2 regulates microRNA-125a expression in neuroblastomaBrain and behaviorFebruary 1, 2022Liu D, Tang X, Huang Z, Wen J, Zhou Y.antibody in the blocking solution for 1 h at room temperature. The monoclonal antibody β‐Tubullin III (ab52623, RRID: AB 869991, 1:500; Abcam, Cambridge, MA, USA) was used as the primary antibody and Alexa Fluor‐conjugated

(17) Shikonin Attenuates Cochlear Spiral Ganglion Neuron Degeneration by Activating Nrf2-ARE Signaling PathwayFrontiers in molecular neuroscienceJanuary 1, 2022Du H, Zhou X, Shi L, Xia M, Wang Y, Guo N, Hu H, Zhang P, Yang H, Zhu F, et al.polyclonal anti-Nrf2 antibody (Cat. No. sc-173), anti-NeuN (Cat. No. ab177487), anti-Tuj1 (Cat. No. ab52623) and anti-Nestin (Cat. No. ab254048) was from Abcam (Cambridge, United Kingdom). Rabbit polyclonal anti-LC3B

(18) Multiplex immunohistochemistry reveals cochlear macrophage heterogeneity and local auditory nerve inflammation in cisplatin-induced hearing lossFrontiers in neurologyJanuary 1, 2022Bedeir MM, Ninoyu Y, Nakamura T, Tsujikawa T, Hirano S.(37) and β3-tubulin marker for investigating neuronal damage (rabbit monoclonal EP1569Y, Abcam, Cat # ab52623, RRID # AB_869991, 1:300) (38). The secondary antibodies included anti-goat antibody and Histofine Simple

(19) LINC00115 promotes stemness and inhibits apoptosis of ovarian cancer stem cells by upregulating SOX9 and inhibiting the Wnt/β-catenin pathway through competitively binding to microRNA-30aCancer Cell InternationalDecember 1, 2021Rui Hou, Luo Jiangfilm/average gray value of the target strip to be tested). GAPDH (1:10,000, ab8245) or β-tubulin (1:1000, ab52623, Abcam) was selected as a reference. The relative level of the protein was calculated as the gray value

(20) A neuroanatomical basis for electroacupuncture to drive the vagal–adrenal axisNatureOctober 28, 2021Shenbin Liu, Zhifu Wang, Yangshuai Su, Lu Qi, Wei Yang, Mingzhou Fu, Xianghong Jing, Yanqing Wang, Qiufu Mawas performed as previously described11 with the following antibodies: rabbit anti-TUBB3 (1:1,000, ab52623, Abcam); rabbit anti-Fos (1:500, ABE457, Millipore); goat anti-ChAT (1:500, AB144P, Millipore); rabbit

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7/29/2019Abcam Beta III Tubulin Antibody Works Well for ImmunofluorescenceDr. Efstathios StamatiadesExcellent staining of beta 3 tubulin+ neurons.

Anti-beta III Tubulin antibody [EP1569Y] from Abcam

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Anti-beta III Tubulin antibody [EP1569Y] from Abcam

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