Fig 1: Discovery of bicistronic transcripts in quiescent NSCs. (A) Visualization of one of the bicistronic Ech1–Lgals4 transcripts in quiescent NSCs performed by using an Integrated Genomic Viewer (top) and a UCSC Genome Browser (bottom). Novel splice-junction spanning reads were detected from partial 3'UTR of the Ech1 transcript into partial 5'UTR of the Lgals4 transcript (red lines) in RNA from a single cell in quiescent NSC. (B) Visualization of the other bicistronic Ech1-Lgals4 transcript in quiescent NSCs by using an Integrated Genomic Viewer (top) and a UCSC Genome Browser (bottom). Novel splice-junction spanning reads were detected from partial 3'UTR of the Ech1 transcript into a 77 bp 5' of intergenic sequence between Ech1 and Lgals4 transcripts and from a 77 bp 3' of intergenic sequences between Ech1 and Lgals4 transcripts into partial 5'UTR of the Lgals4 transcript (red ellipses) in RNA from a single cell in quiescent NSCs. (C) and (D) Sanger sequencing of reverse transcriptase polymerase chain reaction products from mouse SVZ samples confirms the existence of bicistronic transcripts illustrated in (A) and (B), respectively. (E) Western blot of Ech1 and Lgals4 protein expression in HEK293T transfected with the bicistronic Ech1–Lgals4 transcript constructs.