Goat Anti-Rabbit IgG-HRP 4030-05 from SouthernBiotech

Product Specs
ItemGoat Anti-Rabbit IgG-HRP
CompanySouthernBiotech
Price Pricing Info Supplier Page View Company Product Page
Catalog Number4030-05
ApplicationsWB, ELISA, ICC, IHC-fr, IHC-p
ReactivityRabbit (Rb)
FormatLiquid - 50% Glycerol/50% Phosphate buffered saline, pH 7.4
ClonalityPolyclonal
ConcentrationLot specific
PurityPooled antisera from goats hyperimmunized with rabbit IgG; purified by affinity chromatography on pooled rabbit IgG covalently linked to agarose; reacts with the heavy chain of rabbit IgG; cross-adsorbed against rabbit IgM for minimal reactivity
ImmunogenRabbit IgG
Research AreaImmunology, Secondary Antibodies
Antibody TypeSecondary Antibody
NCBI Gene AliasesIGGC, ighg
HostGoat
IsotypeIgG
TargetIgG
Conjugate/TagHRP (Horseradish Peroxidase)
Quantity1.0 mL

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SouthernBiotech

160 Oxmoor Boulevard

Birmingham, Alabama 35209

United States

Phone: (205) 945-1774
Toll-Free U.S. and Canada: (800) 722-2255
Fax: (205) 945-8768

Company Profile

Website: http://www.southernbiotech.com

(1) RNA-Protein Interactome at the Hepatitis E Virus Internal Ribosome Entry SiteMicrobiology SpectrumAugust 17, 2023Shiv Kumar, Rohit Verma, Sandhini Saha, Ashish Kumar Agrahari, Shivangi Shukla, Oinam Ningthemmani Singh, Umang Berry, Anurag, Tushar Kanti Maiti, Shailendra Asthana, et al.antibody was from Santa Cruz Biotechnology (TX, USA). Goat anti-rabbit IgG-horseradish peroxidase (HRP; 4030-05) was from Southern Biotech (AL, USA). 3-Amino-1,2,4-triazole (3-AT), ortho-nitrophenyl-β-galactoside

(2) IRE1a-Induced FilaminA Phosphorylation Enhances Migration of Mesenchymal Stem Cells Derived from Multiple Myeloma PatientsCellsJuly 26, 2023Da Ros F, Kowal K, Vicinanza C, Lombardi E, Agostini F, Ciancia R, Rupolo M, Durante C, Michieli M, Mazzucato M.Healthcare, Milan, Italy, #GENA931) or anti-rabbit HRP (1:1000, SouthernBiotech, Birmingham, AL, USA, #4030-05), the immunoreactive bands were visualized with Amersham ECL Prime Western Blotting Detection Reagent

(3) NRF2 activators inhibit influenza A virus replication by interfering with nucleo-cytoplasmic export of viral RNPs in an NRF2-independent mannerPLOS PathogensJuly 17, 2023Fakhar H. Waqas, Mahmoud Shehata, Walid A. M. Elgaher, Antoine Lacour, Naziia Kurmasheva, Fabio Begnini, Anders E. Kiib, Julia Dahlmann, Chutao Chen, Andreas Pavlou, et al.Cell signaling technology), β-actin (ab49900, 1:20,000, Abcam). Goat anti-rabbit IgG-HRP (Southern Biotech, catalogue no. 4030–05) was used as secondary antibody. 4OI-XPO1 pull-down assay The alkyne-tagged 4OI-probe (4-OI-alk) was

(4) Structural basis of NINJ1-mediated plasma membrane rupture in cell deathNatureJune 29, 2023Morris Degen, José Carlos Santos, Kristyna Pluhackova, Gonzalo Cebrero, Saray Ramos, Gytis Jankevicius, Ella Hartenian, Undina Guillerm, Stefania A. Mari, Bastian Kohl, et al.1:1,000). Primary antibodies were detected with horseradish peroxidase (HRP)-conjugated goat anti-rabbit (4030-05; Southern Biotech; 1:5,000), HRP-conjugated goat anti-mouse (1034-05; Southern Biotech; 1:5,000 or 12–349;

(5) Acute stress induces long-term metabolic, functional, and structural remodeling of the heartNature communicationsJune 28, 2023Yoganathan T, Perez-Liva M, Balvay D, Le Gall M, Lallemand A, Certain A, Autret G, Mokrani Y, Guillonneau F, Bruce J, et al.(1:1000, Abcam). Blots were then incubated during 2 h with HRP-linked anti-rabbit IgG (1:5000, goat, #4030-05, SouthernBiotech) and anti-mouse IgG (1:5000, light chain binding protein, #sc-516102, Santa Cruz Biotechnology). Protocol

(6) Amino acid positions near the active site determine the reduced activity of human ACOD1 compared to murine ACOD1Scientific ReportsJune 26, 2023Fangfang Chen, Israfil Yalcin, Mingming Zhao, Chutao Chen, Wulf Blankenfeldt, Frank Pessler, Konrad BüssowTBST), followed by incubation with the second antibody, goat anti-rabbit IgG-HRP (Southern Biotech #4030-05, diluted 1:5000 in TBST) for 1 h at room temperature. The bands were visualized by enhanced chemiluminescence

(7) Intranasal or airborne transmission-mediated delivery of an attenuated SARS-CoV-2 protects Syrian hamsters against new variantsNature communicationsJune 9, 2023Stauft CB, Selvaraj P, D'Agnillo F, Meseda CA, Liu S, Pedro CL, Sangare K, Lien CZ, Weir JP, Starost MF, et al.Unbound sandwich antibody was washed off and a 1:4000 dilution of an HRP-conjugated goat anti-rabbit IgG (4030-05, Southern Biotech, Birmingham, Alabama) was added to assay plates. In both IgG and IgA ELISAs, incubation

(8) WNK1 promotes water homeostasis by acting as a central osmolality sensor for arginine vasopressin releaseThe Journal of clinical investigationJune 1, 2023Jin X, Xie J, Yeh CW, Chen JC, Cheng CJ, Lien CC, Huang CL.secondary antibodies (A-11005, A-11001, A-11012) were from Thermo Fisher Scientific. Anti-rabbit IgG-HRP (4030-05, Southernbiotech), anti-mouse IgG-HRP (1030-05, Southernbiotech), anti-goat IgG-HRP (6425-05, Southernbiotech),

(9) The ferroptosis inducing compounds RSL3 and ML162 are not direct inhibitors of GPX4 but of TXNRD1Redox BiologyJune 1, 2023Dorian M. Cheff, Chuying Huang, Karoline C. Scholzen, Radosveta Gencheva, Michael H. Ronzetti, Qing Cheng, Matthew D. Hall, Elias S.J. Arnérsignal was detected using anti-rabbit and anti-mouse HRP-IgG secondary antibodies (Southern Biotech, 4030–05, LOT A2718-MM00 and 1030–05, LOT L3919-XE81B respectively) and ECL detection reagent (Cytiva Amersham).

(10) Variation in CD8 T cell IFNγ differentiation to strains of Toxoplasma gondii is characterized by small effect QTLs with contribution from ROP16Frontiers in Cellular and Infection MicrobiologyMay 23, 2023Angel K. Kongsomboonvech, Laura García-López, Ferdinand Njume, Felipe Rodriguez, Scott P. Souza, Alex Rosenberg, Kirk D. C. Jensenincubated with goat α-rabbit horseradish peroxidase (HRP)-conjugated antibodies (Southern Biotech, cat# 4030-05) at 1:4000 dilution for 1h at room temperature. Membranes were then washed again TBS-T 0.1% three times

(11) Autophagy is a novel pathway for neurofilament protein degradation in vivoAutophagyApril 3, 2023Mala V. Rao, Sandipkumar Darji, Philip H. Stavrides, Chris N. Goulbourne, Asok Kumar, Dun-Sheng Yang, Lang Yoo, James Peddy, Ju-Hyun Lee, Aidong Yuan, et al.antibodies conjugated with HRP (Southern Biotech, goat anti-mouse HRP, 1030–05; goat anti-rabbit HRP, 4030–05), and developed with ECL reagent and immunoreactive bands were quantified using Multi Gauge V3.0 (Fuji

(12) Quantitative and temporal measurement of dynamic autophagy ratesAutophagyApril 3, 2023Nitin Sai Beesabathuni, Soyoon Park, Priya S. Shah5 min each. The membrane was then incubated with 1:5000 goat anti-rabbit IgG-HRP (SouthernBiotech, 4030-05) secondary antibody in 5% Milk TBS-T solution. The membrane was washed twice with TBS-T followed by

(13) Cardiac electrophysiological remodeling associated with enhanced arrhythmia susceptibility in a canine model of elite exerciseeLifeFebruary 23, 2023Alexandra Polyák, Leila Topal, Noémi Zombori-Tóth, Noémi Tóth, János Prorok, Zsófia Kohajda, Szilvia Déri, Vivien Demeter-Haludka, Péter Hegyi, Viktória Venglovecz, et al.diluted 1:1000. This was followed by incubation for 1 hr with Goat anti-Rabbit IgG-HRP (SouthernBiotech, 4030–05, RRID:AB_2687483) secondary antibody at a dilution of 1:8000. Band densities were detected with ECL

(14) RIPK3 controls MAIT cell accumulation during development but not during infectionCell death & diseaseFebruary 11, 2023Patton T, Zhao Z, Lim XY, Eddy E, Wang H, Nelson AG, Ennis B, Eckle SBG, Souter MNT, Pediongco TJ, et al.Kenilworth, NJ, USA; #NA-934; 1/2,000) or goat anti-rat IgG HRP (SouthernBiotech, Birmingham, AL, USA; #4030-05; 1/2,000) as appropriate. For housekeeping, Hsp90 was probed with rabbit anti-mouse Hsp90 (Cell Signalling

(15) The length of uninterrupted CAG repeats in stem regions of repeat disease associated hairpins determines the amount of short CAG oligonucleotides that are toxic to cells through RNA interferenceCell Death & DiseaseDecember 30, 2022Andrea E. Murmann, Monal Patel, Si-Yeon Jeong, Elizabeth T. Bartom, A. Jennifer Morton, Marcus E. PeterCell Signaling #5362). Secondary antibodies for Western blot: Goat anti-rabbit-IgG-HRP (Southern Biotech #SB-4030-05, RRID:AB_2687483). Western blot analysis was performed as recently described [56]. All uncropped blots

(16) Active and Passive Immunization of Syrian Hamsters with An Attenuated SARS-CoV-2 Protects against New Variants of ConcernResearch squareNovember 11, 2022Wang T, Stauft C, Selvaraj P, D'agnillo F, Meseda C, Sangare K, Pedro C, Liu S, Lien C, Weir J, et al.Unbound sandwich antibody was washed off and a 1:4000 dilution of an HRP-conjugated goat anti-rabbit IgG (4030–05, Southern Biotech, Birmingham, Alabama) was added to assay plates. In both IgG and IgA ELISAs, incubation

(17) Intranasal delivery of a rationally attenuated SARS-CoV-2 is immunogenic and protective in Syrian hamstersNat CommunNovember 10, 2022Shufeng Liu, Charles B. Stauft, Prabhuanand Selvaraj, Prabha Chandrasekaran, Felice D'Agnillo, Chao-Kai Chou, Wells W. Wu, Christopher Z. Lien, Clement A. Meseda, Cyntia L. Pedro, et al.Biomedical, Jemison, Alabama) was added at 1:4000 dilution followed by 1:4000 Goat anti-rabbit-HRP (4030-05, Southern Biotech, Birmingham, AL). Statistical analysis One-way ANOVA was used to calculate statistical

(18) LRRC15 inhibits SARS-CoV-2 cellular entry in transPLoS biologyOctober 1, 2022Song J, Chow RD, Peña-Hernández MA, Zhang L, Loeb SA, So EY, Liang OD, Ren P, Chen S, Wilen CB, et al.added on the plate for 2-h incubation. Later, goat anti-rabbit IgG-HRP conjugate (Southern Biotech, #4030–05) diluted (1:3,000) in HBSS was used to detect the bound MERS or SARS-CoV RBD fragment. The reaction

(19) Production of novel SARS‐CoV‐2 Spike truncations in Chinese hamster ovary cells leads to high expression and binding to antibodiesBiotechnology JournalSeptember 1, 2022Shiaki A. Minami, Seongwon Jung, Yihan Huang, Bradley S. Harris, Matthew W. Kenaston, Roland Faller, Somen Nandi, Karen A. McDonald, Priya S. ShahRRID:AB_2857936, Wayne, PA), then 1:6000 or 1:4000 goat anti‐rabbit, HRP secondary antibody (SouthernBiotech Cat# 4030‐05, RRID:AB_2687483) in PBS with 1% BSA for purified or crude proteins, respectively. Plates were developed

(20) The Seroprevalence of Crimean-Congo Hemorrhagic Fever in Wild and Domestic Animals: An Epidemiological Update for Domestic Animals and First Seroevidence in Wild Animals from TurkiyeVeterinary SciencesAugust 29, 2022Canakoglu Nurettin, Berber Engin, Tonbak Sukru, Aktas Munir, Vatansever Zati, Ozdarendeli AykutIgG: 6030-05; Goat-anti-porcine IgG: 6050-05; Rabbit anti-sheep IgG: 6150-05; Goat anti-rabbit IgG:4030-05 acquired from Southern Biotech, Birmingham, AL, USA) in 0.05% PBST at 37 °C for 60 min. This was followed

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5/31/2018IgG Western Blot Antibody J ChalThis antibody was used as a secondary antibody to analyze the distribution of a (rabbit) primary antibody for N-cadherin in mouse embryonic tissue. This secondary antibody conjugated with HRP allowed ... Read Review

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