Fig 1: Localization of ANKRD55 in (A) monocytes (1.024 × 1.024 pixels = 0.131 microns/pixel) and (B–E) moDC by immunofluorescence microscopy. Rightmost graph represents mean ± SEM in monocytes and immature moDC (n = 5 cellular ROIs/group; unpaired t-test). (B) Colocalization of ANKRD55 with CD209, a membrane marker specific for moDC (2.048 × 2.048 pixels = 0.065 microns/pixel). (C) Effect of AM580 and IFN-?/LPS treatment on nuclear ANKRD55 signal in immunofluorescence (1,808 × 1,808 pixels = 0,074 microns/pixel). The diagram on the right provides quantitative analysis of nuclear ANKRD55 immunofluorescence [mean ± SEM; n = 59 cellular ROIs/condition; Kruskal–Wallis test (followed by Dunn’s multiple comparison test)]. (D) Colocalization of ANKRD55 with ALYREF (1.024 × 1.024 pixels = 0.131 microns/pixel). (E) Colocalization of ANKRD55 with HNRNPC (1.024 × 1.024 pixels = 0.131 microns/pixel). ***p = 0.001, ****p = 0.0001.