Anti-Paxillin antibody [Y113] ab32084 from Abcam

Product Specs
ItemAnti-Paxillin antibody [Y113]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab32084
Antibody TypePrimary
CloneY113
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Quantity10 UL, 100 UL
ClonalityMonoclonal
IsotypeIgG
HostRabbit
Conjugate/TagUnconjugated
Gene NamePxn
ValidationKnockOut Validated
ReactivityHuman, Mouse
TargetPaxillin

Add to Compare List

Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) The effect of cyclic stretch on aortic viscoelasticity and the putative role of smooth muscle focal adhesionFront PhysiolAugust 11, 2023Cédric H. G. Neutel, Callan D. Wesley, Guido R. Y. De Meyer, Wim Martinet, Pieter-Jan Guns(Abcam, ab40794), 1:1000 rabbit anti-vinculin (Abcam, ab155120), 1:1000 rabbit anti-Paxillin (Abcam, ab32084), 1:2000 mouse anti-Talin1 (Abcam, ab108480), 1:5000 mouse anti-b-actin (Abcam, ab8226), 1:5000 rabbit

(2) IGFBP2 secretion by mammary adipocytes limits breast cancer invasionScience AdvancesJuly 14, 2023James R. W. Conway, Defne D. Dinç, Gautier Follain, Oona Paavolainen, Jasmin Kaivola, Pia Boström, Pauliina Hartiala, Emilia Peuhu, Johanna Ivaska(Santa Cruz Biotechnology, sc-25285), PPARγ (1:100; PA3-821A, Thermo Fisher Scientific, rabbit), paxillin (Y113; 1:100; ab32084, Abcam), or vimentin (D21H3; 1:100; rabbit, 5741, Cell Signaling Technology). Primary antibodies were

(3) Chromatin accessibility dynamics of neurogenic niche cells reveal defects in neural stem cell adhesion and migration during agingNature AgingJuly 13, 2023Robin W. Yeo, Olivia Y. Zhou, Brian L. Zhong, Eric D. Sun, Paloma Navarro Negredo, Surag Nair, Mahfuza Sharmin, Tyson J. Ruetz, Mikaela Wilson, Anshul Kundaje, et al.(Invitrogen, A12379, 665217; 1:500 dilution), ALCAM/CD166 (Bio-techne, AF1172-SP; 1:40 dilution), PXN (Abcam, ab32084; 1:200 dilution) or cleaved caspase3 (Cell Signaling Technology, 9664T; 1:1,000 dilution) resuspended

(4) The structural basis of the talin-KANK1 interaction that coordinates the actin and microtubule cytoskeletons at focal adhesionsOpen biologyJune 1, 2023Li X, Goult BT, Ballestrem C, Zacharchenko T.(Sigma) for 5 min before being washed three times. The primary antibody rabbit anti-paxillin (clone Y113, ab32084, Abcam) was used at a dilution of 1:200 (in 1% BSA), rabbit anti-liprin α (14175-1-AP, Proteintech)

(5) Arctigenin hinders the invasion and metastasis of cervical cancer cells via the FAK/paxillin pathwayHeliyonJune 1, 2023Dan Liao, Yanyan Liu, Cuifen Li, Bin He, Guanghui Zhou, Yejia Cui, Haohai Huang1:1000, Abcam), FAK (ab40794, 1:2000, Abcam), phospho FAK (p-FAK, ab81298, 1:1000, Abcam), paxillin (ab32084, 1:5000, Abcam), p-paxillin (ab24402, 1:1000, Abcam) and GAPDH (1:10000, ab181602, Abcam). Bounds were

(6) Initiation of fibronectin fibrillogenesis is an enzyme-dependent processCell ReportsMay 30, 2023Shay Melamed, Shelly Zaffryar-Eilot, Elisabeth Nadjar-Boger, Rohtem Aviram, Huaning Zhao, Wesal Yaseen-Badarne, Rotem Kalev-Altman, Dalit Sela-Donenfeld, Oded Lewinson, Sophie Astrof, et al.performed at the end of the immunostaining protocol. Antibodies that were used: anti-total Paxillin (Abcam, ab32084, 1:200); anti-FN (Abcam, ab2413, 1:500); anti-LoxL3 and anti-LoxL2 (produced and kindly gifted by G.

(7) Mesenchymal Migration on Adhesive–Nonadhesive Alternate Surfaces in MacrophagesAdvanced ScienceMay 21, 2023Fulin Xing, Hao Dong, Jianyu Yang, Chunhui Fan, Mengdi Hou, Ping Zhang, Fen Hu, Jun Zhou, Liangyi Chen, Leiting Pan, et al.Invitrogen (A22287, USA). Primary antibodies of α‐tubulin and paxillin were from Abcam (ab7291 and ab32084, USA). Alexa Fluor 647‐conjugated secondary antibodies (Invitrogen) and CF568 succinimidyl ester (Biotium,

(8) SPTBN2 regulates endometroid ovarian cancer cell proliferation, invasion and migration via ITGB4‑mediated focal adhesion and ECM receptor signalling pathwayExperimental and Therapeutic MedicineApril 24, 2023La Yang, Yuanyuan Gutemperature for 2 h. Subsequently, cells were incubated overnight with anti-paxillin antibody (1:50; cat. no. ab32084; Abcam) at 4˚C. Cells were incubated with a FITC-conjugated anti-rabbit IgG secondary antibody (1:5,000;

(9) BAG6 supports stress fiber formation by preventing the ubiquitin-mediated degradation of RhoAMolecular Biology of the CellApril 1, 2023Maho Miyauchi, Reina Matsumura, Hiroyuki Kawaharaanti-CUL3 (Cell Signaling Technology #2759), anti-Rac1 23A8 (Abcam ab33186), anti-Paxillin Y113 (Abcam ab32084), anti-BAG6 (Minami et al., 2010), anti-Flag M2 monoclonal (Sigma F1804), anti-Flag polyclonal (Sigma

(10) Mechanotransduction Impairment in Primary Fibroblast Model of Krabbe DiseaseBiomedicinesMarch 16, 2023Roberta Mezzena, Ambra Del Grosso, Roberto Maria Pellegrino, Husam B. R. Alabed, Carla Emiliani, Ilaria Tonazzini, Marco Cecchiniantibodies: FAK (1:1000; ab40794, Abcam); phospho(Tyr397)-FAK (1:1000; ab4803, Abcam); paxillin (1:5000; ab32084, Abcam); p-paxillin (1:1000; ab75740, Abcam); GAPDH (1:3000; G8795, Sigma Aldrich). Membranes, after

(11) Electrical charge on ferroelectric nanocomposite membranes enhances SHED neural differentiationBioactive MaterialsFebruary 1, 2023Xiaochan Li, Boon Chin Heng, Yunyang Bai, Qianqian Wang, Min Gao, Ying He, Xinwen Zhang, Xuliang Deng, Xuehui Zhang(ab8245, Abcam), MSI1 (ab52865, Abcam), TUBB3 (ab18207, Abcam), FAK (PTK2) (ab40794, Abcam), Paxillin (ab32084, Abcam) and p-Paxillin (ab109547, Abcam). Next, the membranes were washed with TBST for 3 times, and

(12) Substrate stiffness controls proinflammatory responses in human gingival fibroblastsScientific reportsJanuary 24, 2023Tiskratok W, Yamada M, Watanabe J, Kartikasari N, Kimura T, Egusa H.Santa Cruz Biotechnology), 1/200 anti-COL1A1 (NBP1-30054, Novus Biologicals, Centennial, CO, USA), and 1/250 anti-paxillin (ab32084, Abcam) overnight at 4 ℃. After washing with PBS, the cells were incubated with the secondary antibody

(13) Mechanical stress driven by rigidity sensing governs epithelial stabilityNature PhysicsJanuary 23, 2023Surabhi Sonam, Lakshmi Balasubramaniam, Shao-Zhen Lin, Ying Ming Yow Ivan, Irina Pi Pi-Jaumà, Cecile Jebane, Marc Karnat, Yusuke Toyama, Philippe Marcq, Jacques Prost, et al.(24E10- Cell Signaling Technology; DECMA1- Sigma Aldrich, ECCD2, Takara Bio) (1:100) and paxillin (Abcam, Ab32084) (1:100) vinculin was a gift from Glukhova lab (1:2). Anti-mouse, anti-rat, and anti-rabbit secondary

(14) Paxillin tunes the relationship between cell–matrix and cell–cell adhesions to regulate stiffness-dependent dentinogenesisRegenerative BiomaterialsJanuary 17, 2023Mingru Bai, Zhaowei Zhang, Huiyu Chen, Xiaoyu Liu, Jing Xiealkaline phosphatase (ZEN BIO, 381009), GAPDH (Cell Signaling Technology, 5174S), paxillin (Abcam, ab32084), osterix (Abcam, ab209484), β-catenin (Invitrogen, 13-8400) and N-cadherin (Abcam, ab18203). The primary

(15) A Near-Infrared Mechanically Switchable Elastomeric Film as a Dynamic Cell Culture SubstrateBiomedicinesDecember 22, 2022Spiaggia G, Taladriz-Blanco P, Hengsberger S, Septiadi D, Geers C, Lee A, Rothen-Rutishauser B, Petri-Fink A.Novus Biologicals (Centennial, CO, USA). Recombinant rabbit monoclonal Anti-Paxillin antibody ([Y113], ab32084) was acquired from Abcam (Cambridge, UK). Milli-Q (Merck, Darmstadt, Germany) water was used for all

(16) Super-resolution microscopy reveals nanoscale architecture and regulation of podosome clusters in primary macrophagesiScienceDecember 22, 2022Fen Hu, Donglan Zhu, Hao Dong, Ping Zhang, Fulin Xing, Wan Li, Rui Yan, Jun Zhou, Ke Xu, Leiting Pan, et al.of α-tubulin,Abcam,Cat# ab7291; RRID:AB_2241126 Rabbit monoclonal antibodies of paxillin,Abcam,Cat# ab32084; RRID:AB_779033 Purified Non-muscle Myosin Heavy Chain II-A Antibody,BioLegend,Cat# 909801; RRID:AB_2565100 Alexa

(17) Single-molecule characterization of subtype-specific β1 integrin mechanicsNature communicationsDecember 3, 2022Jo MH, Li J, Jaumouillé V, Hao Y, Coppola J, Yan J, Waterman CM, Springer TA, Ha T.(v/v) Triton X-100 for 5 min. Paxillin was sequentially stained with anti-Paxillin antibody (Abcam, ab32084; dilution of 1:200) for 1 h and secondary antibody Alexa Fluor™ 488 goat anti-rabbit IgG (Thermo Fischer Scientific

(18) Programmable integrin and N-cadherin adhesive interactions modulate mechanosensing of mesenchymal stem cells by cofilin phosphorylationNat CommunNovember 11, 2022Zheng Zhang, Baoyong Sha, Lingzhu Zhao, Huan Zhang, Jinteng Feng, Cheng Zhang, Lin Sun, Meiqing Luo, Bin Gao, Hui Guo, et al.Cell Signaling, 14074), anti-RUNX2 (1:1000, rabbit, Cell Signaling, 12556), anti-Paxillin (1:200, rabbit, Abcam, ab32084), anti-Lamin A/C (1:100, mouse, Cell Signaling, 4777), anti-Phospho-Cofilin (1:100, rabbit, Cell Signaling,

(19) Epithelial cell attachment and adhesion protein expression on novel in sol TiO 2 coated zirconia and titanium alloy surfacesJournal of Biomedical Materials Research Part B: Applied BiomaterialsNovember 1, 2022Sini Riivari, Elisa NÃ¤rvÃ¤, Ilkka Kangasniemi, Jaana Willberg, Timo NÃ¤rhiBio‐Rad), integrin β4 (1:200, ab110167, Abcam), vinculin (1:100, V9131, Sigma‐Aldrich), paxillin (1:500, ab32084, Abcam)] were diluted in 30% horse serum in PBS and incubated overnight. Samples were washed three times

(20) Kappa-carrageenan-Functionalization of octacalcium phosphate-coated titanium Discs enhances pre-osteoblast behavior and osteogenic differentiationFrontiers in Bioengineering and BiotechnologyOctober 20, 2022Wei Cao, Jianfeng Jin, Gang Wu, Nathalie Bravenboer, Marco N. Helder, Engelbert A. J. M. Schulten, Rommel G. Bacabac, Janak L. Pathak, Jenneke Klein-Nulend(Invitrogen, Fisher Scientific, Carlsbad, CA, United States) and p-paxillin pTy31 polyclonal rabbit IgG (ab32084, Abcam, Cambridgeshire, United Kingdom). The secondary antibody used was Alexa Fluor-488 goat anti-rat

Write a Review

(1)

3/23/2017Great Rabbit Monoclonal Antibody [Y113] To Paxillin Brian ORourkeWe stained PFA fixed U2OS cells to localize the focal adhesions. We utilized this antibody to compare focal adhesion size in migrating cells.

Anti-Paxillin antibody [Y113] from Abcam

Abcam

Citations (165)

Reviews

Anti-Paxillin antibody [Y113] from Abcam

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map