Fig 1: Impact of mistranslation in the muscle of Rps2 A226Y mutant mice at 9 months of age.Graphs show mean, SEM, and individual data points. ***p < 0.001, **p < 0.01, *p < 0.05, ~p < 0.1. a Heatmap showing the significantly regulated genes (FDR < 0.05) in the KEGG pathway ‘Oxidative Phosphorylation (mmu00190)’. b Heatmap showing the significantly regulated genes (FDR < 0.05) in the KEGG pathway ‘Citrate cycle (TCA cycle) (mmu00020)’. c Western blot and densitometry of OXPHOS Complex 2 (SDHB) and OXPHOS Complex 5 (ATP5A) proteins as indicators of mitochondrial content in 9 months WT and A226Y (N = 5). Tubulin as loading control. d Heatmap showing the significantly regulated genes (p < 0.05) associated with slow-twitch muscle fibers. e Western blots of Serca1 and Serca2 levels, and Tnnc1 and Tnni2 levels, in WT and A226Y mouse skeletal muscle. Tubulin as loading control. Graphs show the densitometry of Serca2/Serca1 and Tnnc1/Tnni2 ratios. All values were first normalized to tubulin as loading control (3 = N = 6). f Heatmap showing expression of key regulators of mitochondrial biogenesis: PGC1a, ESRRA, ESRRB, and ESRRG. Western blot plus densitometry of PGC1a protein level in 9 months WT and A226Y (N = 5). Tubulin as loading control. N = number of independent mice in each comparison.