Fig 1: Fc? receptor II-dependent inhibition of human mast cells and anaphylaxis. (A) In vitro degranulation of anti-PN IgE-sensitized mast cells from humanized mice and inhibition by IgG anti-PN. Anti-CD32 or an isotype control mouse IgG was added 2 h prior to IgG anti-PN. (B) In vitro degranulation of human skin mast cells under the conditions used in (A). (C) Core temperature loss (anaphylaxis) in humanized mice passively sensitized with IgE anti-PN and challenged by PN injection. (D) Serum tryptase levels 1 h post-challenge in humanized mice.
Fig 2: Fc? receptor (Fc?R)IIb expression by mast cells in human intestine and skin. Immunofluorescent staining for mast cell tryptase (green) and FcgRIIb (red) on human intestinal or skin tissue sections in tissue arrays. Mast cells are indicated by green arrows. Larger fields, showing more cells, are presented in Figure S3 in Supplementary Material.
Fig 3: Quantitative PCR analysis of FCGR2A and FCGR2B transcripts in human FceRI+ cells. Data are from 5 to 7 samples per cell type.
Fig 4: Fc? receptor (Fc?R)IIb expression by mast cells and basophils from humanized mice, human blood and human skin. Cells from human samples or humanized mouse organs were assessed for FcgRIIa and FcgRIIb expression by flow cytometry. Each experiment was performed at least three times and a representative histogram is presented.
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