Fig 1: Immunofluorescent labeling for the novel ß-cell-selective protein CHODL. (A–E) Whole mount immunofluorescent labeling of human islets for CHODL and the islet hormones as indicated in the figure labels. The boxed region in (A) is magnified in (B). CHODL: chondrolectin; GCG: glucagon; INS: insulin; SST: somatostatin; PP: pancreatic polypeptide; GHRL: ghrelin; DAPI: 4',6-diamidino-2-phenylindole.
Fig 2: Integration of ATAC-seq data with mRNA-seq, histone marks, FAIRE-seq, and transcription factor binding data for novel a- and ß-cell genes. (A) Venn diagrams indicating the proportion of genes that are differentially expressed in a- or ß-cells (based on mRNA-seq) that have cell type-selective ATAC-seq peaks (identified only in a- or ß-cells, including peaks also identified in acinar cells). (B) Pooled sequencing tracks for the GC locus from a-cell, ß-cell, and acinar cell ATAC-seq data (blue); a-cell and ß-cell H3K4me3 (green) and H3K4me27 (red) ChIP-seq data; whole islet H2A.Z ChIP-seq data (light blue); and whole islet FAIRE-seq data (pink). Cell type-selective peaks are identified in blue below the row labeled “RefSeq Genes”, followed by islet transcription factor ChIP-seq peaks in purple. Promoter regions are indicated with black arrows, and putative enhancer regions are labeled with orange arrows. (C) Pooled sequencing tracks for the CHODL gene. Annotation as in (B).
Supplier Page from Abcam for Anti-CHODL antibody [EPR8757]