Anti-Ubiquitin antibody [EPR8830] ab134953 from Abcam

Product Specs
ItemAnti-Ubiquitin antibody [EPR8830]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab134953
Quantity10 UL, 40 UL, 100 UL
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Conjugate/TagUnconjugated
ClonalityMonoclonal
IsotypeIgG
CloneEPR8830
HostRabbit
Antibody TypePrimary
NCBI Gene AliasesHEL-S-50
Gene NameUBC
ReactivityHuman, Mouse, Rat
TargetUbiquitin

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Website: http://www.abcam.com

(1) Molecular mechanism and therapeutic potential of HDAC9 in intervertebral disc degenerationCell Mol Biol LettDecember 13, 2023Ming Lei, Hui Lin, Deyao Shi, Pan Hong, Hui Song, Bomansaan Herman, Zhiwei Liao, Cao Yanganti-acetyl lysine antibody (1:1000, Abcam, ab190479), or anti-ubiquitin (Ubi) antibody (1:5000, Abcam, ab134953). Histological analysis The IVD degeneration was assessed by Safranin-O/Fast green staining. Briefly,

(2) Inhibition of CARM1‐Mediated Methylation of ACSL4 Promotes Ferroptosis in Colorectal CancerAdvanced ScienceNovember 9, 2023Shengjie Feng, Zejun Rao, Jiakun Zhang, Xiaowei She, Yaqi Chen, Kairui Wan, Haijie Li, Chongchong Zhao, Yongdong Feng, Guihua Wang, et al.antibodies against ACSL4 (22401‐1‐AP) were purchased from Proteintech. Antibodies against ubiquitin (ab134953), TRIM9 (ab300515), TRIM25 (ab167154), TRIM33 (ab300146), RNF25 (ab140514), and Ki67 (ab16667) were

(3) Targeting the up-regulated CNOT3 reverses therapeutic resistance and metastatic progression of EGFR-mutant non-small cell lung cancerCell Death DiscoveryNovember 2, 2023Lin Jing, Meng-En Zhai, Mei-Rui Qian, Yi-Ming Li, Ming-Wei Han, Kun Wang, Wan Huang, Gang Nan, Jian-Li Jiangantibodies against phosphorylated c-Jun (S73), ki67 and ubiquitin were from Abcam (ab30620, Ab16667 and ab134953). The antibodies against ERK1/2 were purchased from Abcam (ab54230) and Cell Signaling Technology (4695).

(4) The potential roles of stress‐induced phosphoprotein 1 and connexin 43 in rats with reperfusion arrhythmiaImmunity, Inflammation and DiseaseOctober 3, 2023Li An, Hong Gao, Yi Zhong, Yanqiu Liu, Ying Cao, Jing Yi, Xiang Huang, Chunlei Wen, Rui Tong, Zhijun Pan, et al.loading buffer. The eluted protein was detected by western blotting with an anti‐ubiquitin antibody (ab134953, 1:2000, Abcam). 2.13 Isolated Langendorff‐perfused heart model in rats Sixteen healthy and clean

(5) 3-Bromopyruvate overcomes cetuximab resistance in human colorectal cancer cells by inducing autophagy-dependent ferroptosisCancer gene therapyOctober 1, 2023Mu M, Zhang Q, Zhao C, Li X, Chen Z, Sun X, Yu J.ab184718), anti-phospho-MLKL (1:1000, ab196436), anti-ERK1/2 (1:1000, ab184699), anti-Ubiquitin (1:1000, ab134953), anti-AMPKα (1:2000, ab32047), anti-p62 (1:1000, ab109012), anti-Beclin1 (1:2000, ab207612) primary

(6) The COPS3-FOXO3 positive feedback loop regulates autophagy to promote cisplatin resistance in osteosarcomaAutophagyJune 1, 2023Niu J, Yan T, Guo W, Wang W, Ren T, Huang Y, Zhao Z, Yu Y, Chen C, Huang Q, et al.monoclonal anti-MAPK1-MAPK3/T-ERK,Cell Signaling Technology,4695 Rabbit monoclonal anti-Ubiquitin,Abcam,ab134953 Normal rabbit IgG,Proteintech,B900610 Rabbit monoclonal anti-SKP2,Cell Signaling Technology,2652 "Goat

(7) E3 ubiquitin ligase RNF180 prevents excessive PCDH10 methylation to suppress the proliferation and metastasis of gastric cancer cells by promoting ubiquitination of DNMT1Clinical EpigeneticsMay 5, 2023Nannan Zhang, Xiaoliang Gao, Qiangqiang Yuan, Xin Fu, Pengliang Wang, Fenglin Cai, Hui Liu, Jing Zhang, Han Liang, Yongzhan Nie, et al.Ubiquitination of substrates was analyzed by SDS-PAGE and western blotting using an anti-ubiquitin antibody (Ab134953; Abcam). Statistical analysis Statistical analyses were performed using SPSS (version 22.0), GraphPad

(8) The NR_109/FUBP1/c-Myc axis regulates TAM polarization and remodels the tumor microenvironment to promote cancer developmentJournal for immunotherapy of cancerMay 1, 2023Zhang C, Wei S, Dai S, Li X, Wang H, Zhang H, Sun G, Shan B, Zhao L.(Proteintech, 24 864–1-AP, China), anti-JTV-1 (Proteintech, 10 424-1-AP, China), anti-Ubiquitin (Abcam, ab134953, USA), anti-CD206 (Abcam, ab64693, USA) and anti-SCARB1 (Proteintech, 21 277–1-AP, China). Protein levels

(9) Crizotinib‐based proteolysis targeting chimera suppresses gastric cancer by promoting MET degradationCancer ScienceMay 1, 2023Jin‐Jiao Chen, Jin‐Mei Jin, Wen‐Jie Gu, Zeng Zhao, Hu Yuan, Yu‐Dong Zhou, Dale G. Nagle, Qiu‐Lei Xi, Xue‐Mei Zhang, Qing‐Yan Sun, et al.were purchased from Cell Signaling Technology. The Abs for c‐Myc (Cat# ab32072, 1:1000), ubiquitin (ab134953, 1:500), IKZF1 (ab191394, 1:1000), IKZF3 (ab139408, 1:1000), and GSPT1 (ab234433, 1:1000) were obtained

(10) Promotive role of USP29-mediated deubiquitination in malignant proliferation of colorectal cancer cells via the KIAA1429/SOX8 axisBosnian Journal of Basic Medical SciencesMay 1, 2023Juncai Li, Jianguo Yang, Zhenzhou Chen, Li Liu, Hao Wang, Qican Deng, Yajun Chen, Yong Que, Zhongxue Fuwith antibody anti-KIAA1429 (ab271136, Abcam), and then immunoblotted with antibody anti-ubiquitin (ab134953, Abcam). Animal experiments Animal experiments were authorized by the Ethics Committee of The First

(11) PDGFBB improved the biological function of menstrual blood-derived stromal cells and the anti-fibrotic properties of exosomesStem Cell Research & TherapyApril 28, 2023Xudong Zhang, Siwen Zhang, Jiarui Qi, Fujie Zhao, Yimeng Lu, Shuyu Li, Shanshan Wu, Pingping Li, Jichun Tan1:1000), SMAD3 (#9513, CST, 1:1000), phospho-SMAD3 (Ser425) (YP0585, Immunoway, 1:1000), Ubiquitin (ab134953, Abcam, Cambridge, MA, USA, 1:1000), β-Tubulin (M20005S, Abmart, 1:2000) and GAPDH (#5174, CST, 1:1000).

(12) Positive Feedback Regulation of Circular RNA Hsa_circ_0000566 and HIF-1α promotes Osteosarcoma Progression and Glycolysis MetabolismAging and diseaseApril 1, 2023Shuying Shen, Yining Xu, Zhe Gong, Teng Yao, Di Qiao, Yizhen Huang, Zhenlei Zhang, Jun Gao, Haonan Ni, Zhanping Jin, et al.Cambridge, UK), anti-GLUT4 antibody ab188317 (1:1000; Abcam, Cambridge, UK), anti-ubiquitin antibody ab134953 (1:1000; Abcam, Cambridge, UK), and anti-β-actin antibody AF2811 (1:1000; Beyotime, Shanghai, China)

(13) Impaired OTUD7A-dependent Ankyrin regulation mediates neuronal dysfunction in mouse and human models of the 15q13.3 microdeletion syndromeMolecular psychiatryApril 1, 2023Unda BK, Chalil L, Yoon S, Kilpatrick S, Irwin C, Xing S, Murtaza N, Cheng A, Brown C, Afonso A, et al.182; IF: 1:1000), chicken anti-MAP2 (AVES labs MAP; IF 1:1000), rabbit anti-Ubiquitin [EPR8830] (Abcam ab134953). Fluorophore-conjugated secondary antibodies were raised in donkey and used at a concentration of 1:1000.

(14) NEDD4L facilitates granulosa cell ferroptosis by promoting GPX4 ubiquitination and degradationEndocrine ConnectionsApril 1, 2023Hong Tang, Xiaomei Jiang, Yu Hua, Heyue Li, Chunlan Zhu, Xiaobai Hao, Minhui Yi, Linxia Liantibody (1:500, Abcam, ab183039), NEDD4L antibody (1:500, Abcam, ab168349), GPX4 antibody (1:500, Abcam, ab134953), UB antibody, and anti-GAPDH (glyceraldehyde-3-phosphate dehydrogenase) (1:5000, Abcam, ab9485). The

(15) Maternal hyperglycemia inhibits pulmonary vasculogenesis during mouse fetal lung development by promoting GβL Ubiquitination-dependent mammalian target of Rapamycin assemblyDiabetology & metabolic syndromeMarch 17, 2023Luo Q, Chai X, Xin X, Ouyang W, Deng F.Affinity Biosciences), rabbit anti-Sin1 (1:1000; PRS4077, Sigma-Aldrich), rabbit anti-ubiquitin (1:500; ab134953, Abcam), mouse anti-GβL (1:500, sc-514,982, Santa Cruz Biotechnology), and HRP-conjugated goat anti-rabbit

(16) Serine/threonine-protein kinase STK24 induces tumorigenesis by regulating the STAT3/VEGFA signaling pathwayJournal of Biological ChemistryMarch 1, 2023Senyan Lai, Dao Wang, Wei Sun, Xiaonian Caop-AKT473 (4060; Cell Signaling Technology), AKT (ab8805; Abcam), and CD31 (ab28364; Abcam), ubiquitin (ab134953; Abcam) were all commercially procured for the present study. The specificity of the antibody was validated

(17) The phenotypic and genotypic features of Chinese patients with oculopharyngeal muscular dystrophyAnnals of Clinical and Translational NeurologyMarch 1, 2023Feng Lin, Kang Yang, Min‐ting Lin, Fu‐ze Zheng, Long Chen, Yuan‐liang Ding, Zhi‐xian Ye, Xin Lin, Ning Wang, Zhi‐qiang Wangrabbit; 1:500), anti‐LC3B (Cell Signaling Technology, no. 83506, mouse; 1:200), anti‐ubiquitin (Abcam, ab134953, mouse; 1:250), dystrophin (NCLdys1 Novocastra, 1:100), anti‐FK2 (Millipore Corporation, 04–263, mouse;

(18) Metabolite asymmetric dimethylarginine (ADMA) functions as a destabilization enhancer of SOX9 mediated by DDAH1 in osteoarthritisScience advancesFebruary 10, 2023Wu Y, Shen S, Chen J, Ni W, Wang Q, Zhou H, Chen J, Zhang H, Mei Z, Sun X, et al.(ER1706-89, HUABIO), anti-NOS3 (ab199956, Abcam), anti–p-NOS3S1177 (ab215717, Abcam), anti-ubiquitin (ab134953, Abcam), anti-USP7 (66514-1-Ig, Proteintech, Rosemont, IL, USA; Western blotting), anti-USP7(sc-137008,

(19) Loss of NDR1/2 kinases impairs endomembrane trafficking and autophagy leading to neurodegenerationLife science allianceFebruary 1, 2023Roşianu F, Mihaylov SR, Eder N, Martiniuc A, Claxton S, Flynn HR, Jalal S, Domart MC, Collinson L, Skehel M, et al.(1:400, GP62-C; Progen), rabbit LC3-B (1:200, 3868; Cell Signaling Technology), rabbit ubiquitin (1:100, ab134953; Abcam), rat LAMP2 (1:300, ab134953; Abcam), mouse transferrin receptor—TfR (1:250, 13-6800; Thermo Fisher Scientific

(20) Centrosomal protein 120 promotes centrosome amplification and gastric cancer progression via USP54-mediated deubiquitination of PLK4iScienceJanuary 20, 2023Zhang C, Ma X, Wei G, Zhu X, Hu P, Chen X, Wang D, Li Y, Ruan T, Zhang W, et al.AB_2180460 Myc,Abcam,Cat#ab32; RRID: AB_303599 HA,Abcam,Cat#ab1424; RRID: AB_301017 Ub,Abcam,Cat#ab134953; RRID: AB_2801561 Flag,Abcam,Cat#ab205606; RRID: AB_2916341 His,Abcam,Cat#ab18184; RRID: AB_444306 pHH3,Proteintech,Cat#66863-1-Ig;

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Anti-Ubiquitin antibody [EPR8830] from Abcam

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Anti-Ubiquitin antibody [EPR8830] from Abcam

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