Fig 1: OCLR scores of hub IR-DEGs in KIRC. OCLR scores of hub IR-DEGs at different expression levels in KIRC, including FGF17 (A), PRKCG (B), SSTR1 (C), and SCTR (D). G1 is IR-DEG down-expression and G2 is up-expression in KIRC.
Fig 2: Expression of these hub genes in human KIRC specimens, adjacent normal tissues, and cell lines. (A–D) qRT-PCR analysis of FGF17 (A), PRKCG (B), SSTR1 (C), and SCTR (D) in KIRC cell lines. GAPDH was used as a loading control. (E–H) qRT-PCR analysis of FGF17 (E), PRKCG (F), SSTR1 (G), and SCTR (H) in paired KIRC tissues (n = 19). (I) Representative images of FGF17, PRKCG, and SSTR1 protein immunochemistry in KIRC tissues compared with adjacent normal kidney tissues. Magnification: ×50, ×200; *p < 0.05, **p < 0.01, ***p < 0.001.
Fig 3: Differential expression of immune checkpoint—related genes in KIRC tissues. (A–D) Comparison of immune checkpoints in different expression levels of IR-DEGs and M stage in KIRC, including FGF17 (A), PRKCG (B), SSTR1 (C), and SCTR (D). G1 is IR-DEG upexpression in non-metastatic KIRC. G2 is IR-DEG downexpression in non-metastatic KIRC.
Fig 4: Relationship of immune cell infiltration with IR-DEG levels in KIRC. Infiltration level of various immune cells under different copy numbers of IR-DEG levels, including SCTR (A), SSTR1 (B), PRKCG (C), and FGF17 (D). Correlation of IR-DEG expression levels with B cell, CD8+ T cell, CD4+ T cell, macrophage, neutrophil, and dendritic cell infiltration levels, including SCTR (E), SSTR1 (F), PRKCG (G), and FGF17 (H).
Fig 5: Screening for independent prognostic genes in KIRC. (A,B) Volcano maps of GSE12606 and GSE47352. (C) Venn diagram of GSE12606, GSE47352, and immune-related genes. (D) GO|KEGG enrichment analysis of IR-DEGs. (E,F) Forest plots of univariate and multivariate Cox regression analysis of IR-DEGs. (G) Based on the GEPIA database for differential expression of the four IR-DEGs. (H–K) Survival analysis of four IR-DEGs, including SCTR (H), SSTR1 (I), PRKCG (J), and FGF17 (K).
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