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Antibodies
Antibodies
Anti-PTEN antibody [Y184]
Anti-PTEN antibody [Y184] from Abcam
Be the first to write a review!
Citations:
(174)
Supplier Page
Supplier Page from
Abcam for
Anti-PTEN antibody [Y184]
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-PTEN antibody [Y184]
Company
Abcam
Price
Supplier Page
View Company Product Page
Catalog Number
ab32199
Antibody Type
Primary
Quantity
10 UL, 40 UL, 100 UL
Clone
Y184
Applications
Western blot
Host
Rabbit
Clonality
Monoclonal
Isotype
IgG
Conjugate/Tag
Unconjugated
Gene Name
PTEN
Validation
KnockOut Validated
Reactivity
Human, Mouse
Target
PTEN
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (174)
(1)
Sub-anaesthetic dose of propofol attenuates mechanical allodynia in chronic post-ischaemic pain via regulation of PTEN/PI3K/IL-6 signalling
Molecular Pain
December 1, 2023
Siu Yi Doreen Leung, Fei Meng, Jingjing Liu, Aijia Jessica Liu, Hei Lui Lhotse Ng, Chi Wai Cheung, Sau Ching Stanley Sau Ching Wong
with primary antibodies at 4°C overnight. The primary antibodies used are anti-
PTEN
[Y184] (
Abcam
#
ab32199
,
1:200
), anti-phosphoPTEN (
Abcam
#
ab195056
,
1:150
), anti-phospho-PI3Kp85α (
Abcam
#
ab182651
,
1:400
),
(2)
Triple‑negative breast cancer cells that survive ionizing radiation exhibit an Axl‑dependent aggressive radioresistant phenotype
Exp Ther Med
August 3, 2023
Jianfeng Ji, Yuqin Ding, Yue Kong, Min Fang, Xiaofu Yu, Xiaojing Lai, Qing Gu
Abcam
), Akt (cat. no.
ab38499
;
1:1,000
;
Abcam
), mTOR (cat. no.
ab2732
;
1:2,000
;
Abcam
), PTEN (cat. no.
ab32199
;
1:5,000
;
Abcam
), or GAPDH (cat. no.
ab8245
;
1:2,000
;
Abcam
), followed by
incubation
with the relevant
(3)
Interactions of EGFR/PTEN/mTOR-Pathway Activation and Estrogen Receptor Expression in Cervical Cancer
Journal of Personalized Medicine
July 26, 2023
Thomas Bartl, Christoph Grimm, Robert M. Mader, Christoph Zielinski, Gerald Prager, Matthias Unseld, Merima Herac-Kornauth
Cell Signaling
,
clone
:
2976
49F9, dilution
1:50
), and anti-PTEN monoclonal antibody (source:
Abcam
,
clone
:
Ab32199
, Cambridge, UK, dilution
1:50
) according to the manufacturer specifications (Supplementary Figure S1a–f).
(4)
In Situ Nitric Oxide Gas Nanogenerator Reprograms Glioma Immunosuppressive Microenvironment
Advanced Science
June 1, 2023
Yang Liu, Lin Cui, Xiao Wang, Weiling Miao, Yongxu Ju, Tiandong Chen, Huiting Xu, Ning Gu, Fang Yang
ab150078
), anti p62 antibody (
Abcam
; catalogue no:
ab109012
), anti PTEN antibody (
Abcam
; catalogue no:
ab32199
), anti‐Calreticulin antibody (
Abcam
; catalogue no:
ab22683
), anti GAPDH antibody (
Abcam
; catalogue no:
(5)
GATA6-AS1 via Sponging miR-543 to Regulate PTEN/AKT Signaling Axis Suppresses Cell Proliferation and Migration in Gastric Cancer
Mediators of Inflammation
May 26, 2023
Yi Jin, Daqing Jiang
polyvinylidene fluoride (PVDF)
membranes
. The diluted primary antibodies against PTEN (
1 : 10,000
,
ab32199
;
Abcam
) and loading control GAPDH (
1 : 1,000
,
ab8245
;
Abcam
) were incubated with the blocked
membranes
(6)
miR-19-3p Targets PTEN to Regulate Cervical Cancer Cell Proliferation, Invasion, and Autophagy
Genetics Research
March 3, 2023
Wei Wang, Lu Liu, Yongjian Tian
(4240GR025, Biofroxx) for 2 h at room temperature, the
membranes
were incubated with anti-PTEN (
1 : 1000
,
ab32199
,
Abcam
) and anti-GAPDH (
1 : 1000
.
ab9485
,
Abcam
) antibodies at 4°C overnight. After washing 3 times
(7)
Epigallocatechin-3-
O
-gallate promotes extracellular matrix and inhibits inflammation in IL-1β stimulated chondrocytes by the PTEN/miRNA-29b pathway
Pharmaceutical Biology
December 31, 2022
Dong Yang, Guanghua Cao, Xiaorong Ba, Haibo Jiang
(C)-caspase-3,Rabbit
anti-cleaved caspase-3
antibody,
ab2302
,
Abcam
,
1:500
PTEN,Rabbit anti-PTEN antibody,
ab32199
,
Abcam
,"
1:10,000
" GAPDH,Rabbit anti-GAPDH antibody,
ab181602
,
Abcam
,"
1:10,000
" List of primary antibodies
(8)
Mesenchymal stem cell-originated exosomal circDIDO1 suppresses hepatic stellate cell activation by miR-141-3p/PTEN/AKT pathway in human liver fibrosis
Drug Delivery
December 31, 2022
Li Ma, Junfeng Wei, Yanli Zeng, Junping Liu, Erhui Xiao, Yuehua Kang, Yi Kang
ab109201
), CD63 (
1:2000
,
ab68418
), α-SMA (
1:2000
,
ab5694
), collagen I (
1:2000
,
ab34710
), PTEN (
1:10,000
,
ab32199
), and β-actin (
1:1000
,
ab6276
), were obtained from
Abcam
(Cambridge, UK), and protein kinase B (
AKT
)
(9)
Effect of CTP-mediated PTEN on 5637 bladder cancer cells and the underlying molecular mechanism
BMC Urology
December 10, 2022
Bei Yu, Yuan Huang, Yue Yang, Haifeng Hu, Jin Yang
purchased from Invitrogen Corporation. Rabbit anti-PTEN monoclonal antibody was purchased from
Abcam
(
ab32199
).
Goat anti-rabbit IgG-HRP
was purchased from
Santa Cruz Biotechnology, Inc
. 5637
bladder cancer
cells
(10)
Morin Inhibits Dox-Induced Vascular Inflammation By Regulating PTEN/AKT/NF-κB Pathway
Inflammation
December 1, 2022
Yu J, Qi HL, Zhang H, Zhao ZY, Jing-Zhao, Nie ZY.
(
1:1000
, Proteintech, 21,865–1-AP), anti-TNF-α (
1:1000
, Proteintech, 6029–1-Ig), anti-PTEN (
1:1000
,
Abcam
,
ab32199
),
anti-pan-AKT
(
1:1000
,
Abcam
,
ab8805
),
anti-pan-AKT (phospho T308
;
1:1000
,
Abcam
,
ab38449
), and
1:1000
(11)
SIRT1 restoration enhances chondrocyte autophagy in osteoarthritis through PTEN-mediated EGFR ubiquitination
Cell Death Discovery
December 1, 2022
Qunshan Lu, Peilai Liu, Zhuang Miao, Desu Luo, Songlin Li, Mei Lu
Diluted primary antibodies of anti-
SIRT1
(
1:1000
,
ab110304
,
Abcam
, Cambridge, UK), anti-
PTEN
(
1:1000
,
ab32199
,
Abcam
), anti-Aggrecan (
1:1000
,
ab36861
,
Abcam
), anti-Collagen II (
1:1000
,
ab34712
,
Abcam
), anti-MMP-13
(12)
Potential pathogenic mechanism of type 1 X-linked lymphoproliferative syndrome caused by a mutation of SH2D1A gene in an infant: A case report
Medicine (Baltimore)
October 14, 2022
Yanchun Wang, Yan Wang, Weimin Lu, Lvyan Tao, Yang Xiao, Yuantao Zhou, Xiaoli He, Yu Zhang, Li Li
Anti-PI3 Kinase p110δ (
Abcam
,
ab109006
); Anti-PI3 Kinase p85α (
Abcam
,
ab191606
); Anti-PTEN (
Abcam
,
ab32199
); Anti-
AKT
(
CST
,
9272s
); Anti-phospho-AKT (
CST
,
9271s
); Anti-mTOR (
Abcam
,
ab2732
). 2.5. Prediction
(13)
Cross Brain–Gut Analysis Highlighted Hub Genes and LncRNA Networks Differentially Modified During Leucine Consumption and Endurance Exercise in Mice with Depression-Like Behaviors
Molecular Neurobiology
July 1, 2022
Navid Abedpoor, Farzaneh Taghian, Fatemeh Hajibabaie
162–0176
, USA). The primary antibodies used were rabbit KDR antibody (
Abcam
,
ab11939
, USA), anti-PTEN (
ab32199
,
Abcam
), and mouse anti-GAPDH antibody (
Santa Cruz
,
sc-32233
).
Membranes
were incubated with primary
(14)
Repeated cell sorting ensures the homogeneity of ocular cell populations expressing a transgenic protein
PLOS ONE
March 25, 2022
Tsan-Chi Chen, Shu-Wen Chang
Santa Cruz Biotechnology
, Santa Cruz, CA), GFP (
sc-8334
; rabbit polyclonal;
Santa Cruz Biotechnology
), PTEN (
ab32199
; rabbit monoclonal;
Abcam
),
Thy-1
(
ab133350
; rabbit monoclonal;
Abcam
), or ZO-1 (
ab96587
; rabbit monoclonal;
(15)
The phosphatase and tensin homolog gene inserted between NP and P gene of recombinant New castle disease virus oncolytic effect test to glioblastoma cell and xenograft mouse model
Virology journal
January 29, 2022
Jang SH, Jung BK, An YH, Jang H.
Waltham, MA, USA). Blocking was performed by 1% BSA for 1 h. PTEN monoclonal antibody (
1:1000
cat. No.
ab32199
Abcam
, Cambridge, United Kingdom), Phospho-AKT1 (Ser473) monoclonal antibody (
1:1000
cat. No.
44-621G
(16)
Identification of a Cowden syndrome patient with a novel PTEN mutation and establishment of patient-derived induced pluripotent stem cells
In Vitro Cellular & Developmental Biology - Animal
January 3, 2022
Fumitaka Obayashi, Atsuko Hamada, Sachiko Yamasaki, Taku Kanda, Shigeaki Toratani, Tetsuji Okamoto
Antibody,Cat. no,Company PTEN,
ab32199
,
Abcam
p-PTEN(Ser380),#
9511
,
Cell Signaling Technology
AKT
,
sc-5298
,
SANTA CRUZ
p-AKT(Ser437),#
9271
,
Cell Signaling Technology
(17)
Disruption of the pentraxin 3/CD44 interaction as an efficient therapy for triple-negative breast cancers
Clinical and translational medicine
January 1, 2022
Hsiao YW, Chi JY, Li CF, Chen LY, Chen YT, Liang HY, Lo YC, Hong JY, Chuu CP, Hung LY,
et al
.
NF‐κB
p65
(#
3033
;
Cell Signaling
), Phospho‐p38 MAPK (Thr180/Tyr182) (#
9211
;
Cell Signaling
), PTEN (
ab32199
;
Abcam
), p38 MAPK (#
9212
;
Cell Signaling
), Phospho‐SAPK/JNK (Thr183/Tyr185) (#
4668
;
Cell Signaling
),
(18)
miR-21 regulates osteogenic and adipogenic differentiation of BMSCs by targeting PTEN
J Musculoskelet Neuronal Interact
December 2, 2021
Yongtao Zhou, Hongwang Qiao, Lili Liu, Ping Dong, Fangxu Zhu, Jiawen Zhang, Liping Liu, Li Liu
covered with 5% skimmed milk powder, with a supplement of primary antibody target gene PTEN (
1:1000
,
ab32199
,
Abcam
, UK) or internal reference gene β-actin (
ab8226
,
Abcam
, UK) respectively for
hybridization
at
(19)
Long non-coding RNA TUG1 knockdown promotes autophagy and improves acute renal injury in ischemia-reperfusion-treated rats by binding to microRNA-29 to silence PTEN
BMC Nephrology
December 1, 2021
Zhiquan Xu, Xiaoyan Huang, Qiuyu Lin, Wei Xiang
µg/mL Beclin-1,"
ab207612
,
Abcam
",1/2000 LC3II,"
ab48394
,
Abcam
",2 µg/mL LC3I,"
ab192890
,
Abcam
",1/2000 PTEN,"
ab32199
,
Abcam
","1/10,000" GAPDH,"
ab181602
,
Abcam
","1/10,000" Antibodies used in experiment Note:
Bcl-2
,
(20)
PTEN alleviates maladaptive repair of renal tubular epithelial cells by restoring CHMP2A-mediated phagosome closure
Cell death & disease
November 16, 2021
Wang H, Wang Y, Wang X, Huang H, Bao J, Zhong W, Li A.
performed as previously described [17]. The primary antibodies used were as follows: anti-
PTEN
(
Abcam
Cat#
ab32199
, RRID:AB_777535;
Cell Signaling Technology
Cat#
9188
, RRID:AB_2253290;
Proteintech
Cat#
60300-1-Ig
,
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Anti-PTEN antibody [Y184] from Abcam
Supplier Page
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Product Specs
Company Info
Citations
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