Anti-Perforin antibody [B-D48] ab47225 from Abcam

Product Specs
ItemAnti-Perforin antibody [B-D48]
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab47225
Antibody TypePrimary
HostMouse, Nude mouse
Quantity100 UG
IsotypeIgG1
ClonalityMonoclonal
ApplicationsFlow Cytometry, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunohistochemistry (Frozen sections), Western blot
CloneB-D48
Conjugate/TagUnconjugated
Concentration1 mg/ml
Research AreaImmuno-oncology
Gene NamePRF1
ReactivityHuman
TargetPerforin

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) MicroRNA-20a Mediates the Cytotoxicity of Natural Killer Cells in Endometriosis via ERG/HLX/STAT4/Perforin AxisSSRN Electronic JournalMarch 31, 2022Li-Juan Chen, Bin Hu, Zhi-Qiang Han, Jian Ni, Yong-Ming Zhou, Xue-Xing Chen, Hao Zhou(ab19870, 1:500, Abcam), PSTAT4 (Y693) (ab28815, 1:1000, Abcam), STAT4 (ab235946, 1:1000, Abcam), Perforin (ab47225, 1:100, Abcam) and GADPH (ab37168, 1:1000, Abcam) at 4°C overnight, followed by 3 TBST washes. The membrane

(2) Deep spatial profiling of human COVID-19 brains reveals neuroinflammation with distinct microanatomical microglia-T-cell interactionsImmunityJuly 13, 2021Schwabenland M, Salié H, Tanevski J, Killmer S, Lago MS, Schlaak AE, Mayer L, Matschke J, Püschel K, Fitzek A, et al.B27",BioLegend,Cat #506502; RRID: AB_315435 Mouse monoclonal anti-Perforin antibody [B-D48] - Azide free,Abcam,Cat# ab47225; RRID: AB_2169084 "Mouse monoclonal Purified NA/LE Mouse Anti-Human CD152 (CTLA-4), Clone BNI3",BD,Cat#

(3) MiRNA-340-5p mediates the functional and infiltrative promotion of tumor-infiltrating CD8+ T lymphocytes in human diffuse large B cell lymphomaJournal of Experimental & Clinical Cancer ResearchDecember 1, 2020Yangyang Xu, Zhenchuan Liu, Lixin Lv, Ping Li, Bing Xiu, Wenbin Qian, Aibin Liang(Abcam, ab231036), anti-IL-2 (Abcam, ab92381), anti-TNF-α (Abcam, ab270264), anti-perforin (Abcam, ab47225), anti-Granzyme B (BOSTER, A00353), anti-perforin (Abcam, ab16074). The following secondary antibodies

(4) Human Tumor-Infiltrating MAIT Cells Display Hallmarks of Bacterial Antigen Recognition in Colorectal CancerCell reports. MedicineJune 23, 2020Li S, Simoni Y, Becht E, Loh CY, Li N, Lachance D, Koo SL, Lim TP, Tan EKW, Mathew R, et al.(clone PCH101),Thermofisher,"Cat# 13-4776-82, RRID:AB_763539" 175 perforin (clone B-D48),Abcam,"Cat# ab47225, RRID:AB_2169084" 176 - CD19 (clone HIB19),Biolegend,"Cat# 302202, RRID:AB_314232" 176 - CD39 (clone

(5) Costs in Different States of Breast CancerAnticancer ResearchJanuary 1, 2019EIJA ROINE, NIILO FÄRKKILÄ, HARRI SINTONEN, KIMMO TAARI, RISTO P. ROINE, TIINA SAARTOanti-GZMA antibody (ab209205; Abcam, Cambridge, UK) diluted 1:100; monoclonal mouse anti-PRF1 antibody (ab47225; Abcam) diluted 1:100; and, monoclonal mouse anti-CD8 antibody (M7103; Dako, Glostrup, Denmark), diluted 1:100.

(6) Cytolytic Activity (CYT) Score Is a Prognostic Biomarker Reflecting Host Immune Status in Hepatocellular Carcinoma (HCC)Anticancer ResearchDecember 1, 2018HIROAKI WAKIYAMA, TAKAAKI MASUDA, YUSHI MOTOMURA, QINGJIANG HU, TARO TOBO, HIDETOSHI EGUCHI, KATSUMI SAKAMOTO, MASAKAZU HIRAKAWA, HIROSHI HONDA, KOSHI MIMORIanti-GZMA antibody (ab209205; Abcam, Cambridge, UK) diluted 1:100; monoclonal mouse anti-PRF1 antibody (ab47225; Abcam) diluted 1:100; and, monoclonal mouse anti-CD8 antibody (M7103; Dako, Glostrup, Denmark), diluted 1:100.

(7) Epigenomic-Guided Mass Cytometry Profiling Reveals Disease-Specific Features of Exhausted CD8 T CellsImmunityMay 15, 2018Bengsch B, Ohtani T, Khan O, Setty M, Manne S, O'Brien S, Gherardini PF, Herati RS, Huang AC, Chang KM, et al.E12.2.H7),Biolegend,RRID:AB_10960742 Cat#329920 "Anti-Perforin, clone B-D48",Abcam,RRID:AB_2169084 Cat#ab47225 "Anti-Ptger2, polyclonal AB9472",Merck Millipore,RRID:AB_2174912 Cat#AB9472 "Anti-T-bet, clone 4B10,

(8) Deep immune profiling by mass cytometry links human T and NK cell differentiation and cytotoxic molecule expression patternsJournal of immunological methodsFebruary 1, 2018Bengsch B, Ohtani T, Herati RS, Bovenschen N, Chang KM, Wherry EJ.

(9) NaHCO3 enhances the antitumor activities of cytokine-induced killer cells against hepatocellular carcinoma HepG2 cellsOncology LettersNovember 1, 2016Ya Hong Yuan, Chun Fang Zhou, Jiang Yuan, Li Liu, Xing Rong Guo, Xiao Li Wang, Yan Ding, Xiao Nan Wang, Dong Sheng Li, Han Jun Tudilution, 1:50; Abcam, Cambridge, MA, USA) and mouse anti-human perforin monoclonal antibody (cat. no. ab47225; dilution, 1:50; Abcam) were used for immunostaining. Horseradish peroxidase-conjugated goat anti-rabbit

(10) Recruitment of Grb2 and SHIP1 by the ITT-like motif of TIGIT suppresses granule polarization and cytotoxicity of NK cellsCell Death & DifferentiationMarch 1, 2013S Liu, H Zhang, M Li, D Hu, C Li, B Ge, B Jin, Z Fanfrom as follows: anti-human TIGIT PE (eBioscience, San Diego, CA, USA); anti-PVR; Perforin antibody (ab47225) from TIGIT inhibits NK cell activity S Liu et al 463 Abcam (Cambridge, UK); anti-phosphotyrosine 4G10 Platinum

(11) The PP2A inhibitor SET regulates granzyme B expression in human natural killer cellsBloodFebruary 24, 2011Rossana Trotta, David Ciarlariello, Jessica Dal Col, Hsiaoyin Mao, Li Chen, Edward Briercheck, Jianhua Yu, Jianying Zhang, Danilo Perrotti, Michael A Caligiuri

(12) Enrichment and analysis of secretory lysosomes from lymphocyte populationsBMC immunologyJuly 29, 2009Schmidt H, Gelhaus C, Lucius R, Nebendahl M, Leippe M, Janssen O.

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Anti-Perforin antibody [B-D48] from Abcam

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Anti-Perforin antibody [B-D48] from Abcam

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