Anti-STAT5a antibody [E289] ab32043 from Abcam

Product Specs
ItemAnti-STAT5a antibody [E289]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab32043
Antibody TypePrimary
HostRabbit
IsotypeIgG
Quantity10 UL, 40 UL, 100 UL
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunoprecipitation, Western blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunoprecipitation, Western blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunoprecipitation, Western blot
ClonalityMonoclonal
CloneE289
Conjugate/TagUnconjugated
Research AreaImmuno-oncology
Gene NameSTAT5A
ReactivityHuman, Mouse, Rat
TargetSTAT5a

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) The Kruppel-like factor 4-signal transducer and activator of transcription 5A axis promotes pancreatic fibrosis in mice with caerulein-induced chronic pancreatitisExperimental AnimalsAugust 7, 2023Xiaoxiang WANG, Lan YU, Yao CHEN, Xing XIONG, Hongmei RANversion 1.52 software. Primary antibodies included: KLF4 (1:1,000, ab214666, Abcam), STAT5 (1:1,000, ab32043, Abcam), Collagen I (1:1,000, ab260043, Abcam), Collagen III (1:5,000, ab7778, Abcam), alpha-smooth muscle

(2) TNFα Effects on Adipocytes Are Influenced by the Presence of Lysine Methyltransferases, G9a (EHMT2) and GLP (EHMT1)BiologyApril 30, 2023Able AA, Richard AJ, Stephens JM.from R&D Systems (Minneapolis, MN, USA). Anti-H3K9me1 (ab8896; rabbit polyclonal) and anti-STAT5A (ab32043; rabbit monoclonal) antibodies were purchased from Abcam (Cambridge, UK). Anti-Adiponectin (PA1-054;

(3) Targeting the STAT5A/IDO1 axis overcomes radioresistance and reverses the immunosuppressive tumor microenvironment in NSCLCInternational journal of oncologyJanuary 1, 2023Yang Y, Zheng X, Ni P, Li D, Dan Q, Wang X, Wang Y, Sun Y, Liu K, Dong Z, et al.anti-mouse CD8 (1:200; cat. no. bs-0648R; Bioss antibodies, Inc.), rabbit anti-STAT5A (1:200; cat. no. ab32043; Abcam, Inc.), rabbit anti-Ki67 (1:400; cat. no. ab15580; Abcam); and rabbit anti-human FOXP3 (1:100;

(4) Leukemia inhibitory factor is a therapeutic target for renal interstitial fibrosisEBioMedicineDecember 1, 2022Xu S, Yang X, Chen Q, Liu Z, Chen Y, Yao X, Xiao A, Tian J, Xie L, Zhou M, et al.ab30648, RRID: AB_779091), anti-p-STAT5a (Abcam Cat# ab128896, RRID: AB_11150987), anti-STAT5a (Abcam Cat# ab32043, RRID: AB_778107), anti-p-STAT5b (Abcam Cat# ab52211, RRID: AB_2196931), anti-STAT5b (Abcam Cat# ab178941,

(5) A highly selective JAK3 inhibitor is developed for treating rheumatoid arthritis by suppressing γc cytokine–related JAK-STAT signalScience AdvancesAugust 19, 2022Chengjuan Chen, Yuan Yin, Gaona Shi, Yu Zhou, Shuai Shao, Yazi Wei, Lei Wu, Dayong Zhang, Lan Sun, Tiantai ZhangCell Signaling Technologies, 9139), p-STAT3 (1:1000; Cell Signaling Technologies, 9145), STAT5 (1:1000; Abcam, ab32043), p-STAT5 (1:1000; Cell Signaling Technologies, 4322), STAT6 (1:1000; Abcam, ab44718), p-STAT6 (1:1000;

(6) Oncostatin M Induces Lipolysis and Suppresses Insulin Response in 3T3-L1 AdipocytesInternational journal of molecular sciencesApril 23, 2022Bailey JL, Hang H, Boudreau A, Elks CM.1,3194,1:1000 (5% NFDM) anti-pIRS1 (S612),Cell Signaling,2386,1:1000 (5% BSA-TBST) 2 anti-STAT5A,Abcam 3,ab32043,1:1000 (1% BSA- TBST) anti-p-p66 (S36),Millipore Sigma,566807,1:500 (1% BSA-TBST) anti-Shc1,R&D Systems,MAB7129,1:500

(7) Sulforaphane ameliorates amyloid-β-induced inflammatory injury by suppressing the PARP1/SIRT1 pathway in retinal pigment epithelial cellsBioengineeredJanuary 4, 2022Song Wang, Yu-jie TangLipofectamine 2000 was provided by Sigma-Aldrich. The antibodies against cleaved caspase-3 (catalog no. #ab32043), cleaved caspase-3 (ab2324), PARP1 (ab32064), SIRT1 (ab189494), and β-actin (ab7817) were obtained

(8) Elevated Neddylation Pathway Promotes Th2 Cells Infiltration by Transactivating STAT5A in Hepatocellular CarcinomaFrontiers in OncologyNovember 5, 2021Lisha Zhou, Luyi Zhang, Siyuan Chen, Dongsheng Sun, Jianhua Qugels. Western blot images were captured by Amersham Imager 680 (GE Healthcare). Antibody for STAT5A (ab32043) was purchased from Abcam. β-actin (M1210-2) was purchased from HuaBio. Statistical Analysis R 3.6.3

(9) HBeAg mediates inflammatory functions of macrophages by TLR2 contributing to hepatic fibrosisBMC medicineOctober 15, 2021Xie X, Lv H, Liu C, Su X, Yu Z, Song S, Bian H, Tian M, Qin C, Qi J, et al.(ab268021), CD68 (ab213363), FOXO3a (ab109629), Smad3 (ab40854), Smad2 (ab33875), p-STAT5 (ab32364), STAT5 (ab32043), p-STAT3 (ab76315), STAT3(ab68153), p-mTOR (ab109268), JNK (ab179461), p-AKT1 (ab108266), and AKT1

(10) Histone Deacetylase Inhibitor Trichostatin A Reduces Endothelial Cell Proliferation by Suppressing STAT5A-Related Gene TranscriptionFrontiers in OncologySeptember 23, 2021Yize Li, Yongmei Zhao, Hongyan Peng, Jing Zhang, Lun Bo, Lei Wen, Wenchao Liu, Wendong Bai, Hongmei Zhangregulatory subunit 1 (CKS1B) (cat #ab72639), anti-NDC80 (cat #ab186839) antibodies, and anti-STAT5A (cat #ab32043) antibodies were obtained from Abcam (Cambridge, UK); anti-STAT5A (cat #94205, ChIP grade) was obtained

(11) STAT5a Confers Doxorubicin Resistance to Breast Cancer by Regulating ABCB1Frontiers in OncologyJuly 15, 2021Zhaoqing Li et al.IL, USA) for 1 h at room temperature and incubated overnight with primary antibodies against STAT5a (ab32043, Abcam, Cambridge, MA, USA), STAT5b (ab178941; Abcam), p-STAT5 (Tyr694) (ab32364, Abcam), cleaved PARP

(12) Loss of STAT5A promotes glucose metabolism and tumor growth through miRNA-23a-AKT signaling in hepatocellular carcinomaMolecular oncologyFebruary 1, 2021Jiang Y, Tao Y, Zhang X, Wei X, Li M, He X, Zhou B, Guo W, Yin H, Cheng S.poly(vinylidene fluoride) (PVDF, Millipore, Burlington, MA, USA) membranes. Antibodies included STAT5A (Abcam, ab32043), and STAT5A phospho Y694 (Abcam, ab30648), p‐AKT (S473) (CST, 4060S, Danvers, MA, USA), p‐AKT (T308)

(13) Increased SUMO-activating enzyme SAE1/UBA2 promotes glycolysis and pathogenic behavior of rheumatoid fibroblast-like synoviocytesJCI insightSeptember 17, 2020Wang C, Xiao Y, Lao M, Wang J, Xu S, Li R, Xu X, Kuang Y, Shi M, Zou Y, et al.anti-SAE1 (ab38434, Abcam), anti-UBA2 (ab189289, Abcam), anti–SUMO-1 (ab11672, Abcam), and anti-STAT5A (ab32043, Abcam) were purchased from Abcam. Anti-HK2 (2867, Cell Signaling Technology), anti-PFKP (8164, Cell Signaling Technology

(14) Reciprocal fine-tuning of progesterone and prolactin-regulated gene expression in breast cancer cellsMolecular and Cellular EndocrinologyJuly 1, 2020Sean M. Holloran, Bakhtiyor Nosirov, Katherine R. Walter, Gloria M. Trinca, Zhao Lai, Victor X. Jin, Christy R. HaganPrimary antibodies for immunoblotting are as follows: PR (sc-7208; Santa Cruz Biotechnology), STAT5A (ab32043; Abcam), STAT5B (1656; Santa Cruz Biotechnology), Prolactin Receptor (13552; Cell Signaling), and beta-tubulin

(15) LncRNA SNHG17 aggravated prostate cancer progression through regulating its homolog SNORA71B via a positive feedback loopCell Death & DiseaseMay 1, 2020Gaoliang Wu, Chao Hao, Xueliang Qi, Jianqiang Nie, Weimin Zhou, Ji Huang, Qiuming HePVDF with milk, the primary antibodies were added for incubation overnight, including anti-STAT5A (ab32043, Abcam, Cambridge, UK), anti-E-cadherin (ab1416, Abcam), anti-N-cadherin (ab18203, Abcam) and anti-GAPDH

(16) Glucocorticoids paradoxically facilitate steroid resistance in T cell acute lymphoblastic leukemias and thymocytesThe Journal of clinical investigationFebruary 3, 2020Meyer LK, Huang BJ, Delgado-Martin C, Roy RP, Hechmer A, Wandler AM, Vincent TL, Fortina P, Olshen AB, Wood BL, et al.(Thermo Fisher Scientific; 78833). Immunoblotting was performed with the following antibodies: STAT5A (Abcam; ab32043), STAT5B (Abcam; ab178941), GR (Cell Signaling Technology; 12041), p-GR (Ser211) (Cell Signaling Technology;

(17) Tumor suppressor TET2 promotes cancer immunity and immunotherapy efficacyThe Journal of clinical investigationJuly 16, 2019Xu YP, Lv L, Liu Y, Smith MD, Li WC, Tan XM, Cheng M, Li Z, Bovino M, Aubé J, et al.(Proteintech, 510752-AP), STAT3 (Cell Signaling Technology, 9139S), STAT4 (Abcam, ab68156), STAT5a (Abcam, ab32043), STAT5b (Abcam, ab178941), and STAT6 (Abcam, ab32520) were purchased commercially. Gene deletion by

(18) Oncostatin M Mediates Adipocyte Expression and Secretion of Stromal-Derived Factor 1Biology (Basel)March 23, 2019Hardy Hang, Jennifer L. Bailey, Carrie M. ElksImmunoResearch, West Grove, PA, USA) and SuperSignal West Pico PLUS reagents (Thermo Fisher). Anti-STAT5A (E289; ab32043; rabbit monoclonal) and OSMRβ (AF662; goat polyclonal) antibodies were purchased from Abcam and R&D

(19) miR-100 maintains phenotype of tumor-associated macrophages by targeting mTOR to promote tumor metastasis via Stat5a/IL-1ra pathway in mouse breast cancerOncogenesisDecember 19, 2018Wang W, Liu Y, Guo J, He H, Mi X, Chen C, Xie J, Wang S, Wu P, Cao F, et al.Abcam, Cambridge, MA, USA, Cat. No. ab-124962; Stat5a: Abcam, Cat. No. ab178941; Stat5b: Abcam, Cat. No. ab32043; mTOR: CST, Danvers, MA, USA, Cat. No. 2983; p-S6: CST, Cat. No. 4857; Gli1: Abcam, Cat. No. Ab151796;

(20) Inhibition of STAT5A promotes osteogenesis by DLX5 regulationCell death & diseaseNovember 14, 2018Lee KM, Park KH, Hwang JS, Lee M, Yoon DS, Ryu HA, Jung HS, Park KW, Kim J, Park SW, et al.antibodies used for blotting were anti-STAT5 (1:1000, Abcam; ab68465), anti-STAT5A (1:1000, Abcam; ab32043), anti-STAT5B (1:1000, Abcam; ab178941), anti-DLX5 (1:500, Abcam; ab109737), and anti-RUNX2 (1:1000,

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Anti-STAT5a antibody [E289] from Abcam

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Anti-STAT5a antibody [E289] from Abcam

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