Anti-NF-kB p65 (phospho S536) antibody ab28856 from Abcam

Product Specs
ItemAnti-NF-kB p65 (phospho S536) antibody
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab28856
Antibody TypePrimary
HostRabbit
ApplicationsImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
IsotypeIgG
ClonalityPolyclonal
Quantity100 UG
Conjugate/TagUnconjugated
FormatThe antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site
Concentration1 mg/ml
ModificationsPhospho-S536
Gene NamerelA
ReactivityHuman
TargetNF-kB p65

Add to Compare List

Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Cimifugin inhibits adipogenesis and TNF-α-induced insulin resistance in 3T3-L1 cellsOpen Med (Wars)November 29, 2023Xiang Deng, Zhenmin Liu, Siqi Han1:1,000; Abcam), GLUT-4 (ab33780, 1:1,000; Abcam), IRS-1 (ab46800, 1:10,000; Abcam), phospho-P65 (p-P65, ab28856, 1:1,000; Abcam), P65 (ab32536, 1:1,000; Abcam), phospho-ERK (p-ERK, ab201015; Abcam), ERK (ab184699,

(2) EGCG Attenuates CA1 Neuronal Death by Regulating GPx1, NF-κB S536 Phosphorylation and Mitochondrial Dynamics in the Rat Hippocampus following Status EpilepticusAntioxidants (Basel, Switzerland)April 20, 2023Kim JE, Kim TH, Kang TC.MTCO1)",Mouse,Abcam (#ab14705),1:500 (IH) NeuN,Guinea pig,Millipore (#ABN90P),1:1000 (IH) NF-κB S536,Rabbit,Abcam (#ab28856),1:100 (IH) p-DRP1 S616,Rabbit,Cell Signaling (#4494),1:1000 (WB) p-ERK1/2,Rabbit,Millipore (#05-797R),1:100

(3) MUC5B regulates goblet cell differentiation and reduces inflammation in a murine COPD modelRespiratory ResearchDecember 1, 2022Xuan Huang, Weijie Guan, Bin Xiang, Wei Wang, Yanqing Xie, Jinping ZhengBSA (Amresco) (ERK1/2: 1:2,000, CST, 9102S; p-ERK1/2: 1:2,000, CST, 9102S; p-NF-κB: 1:2,000, Abcam, ab28856; GAPDH: 1:8,000, Abcam, ab181602; SPDEF: ab53881, Abcam; STAT6: ab44718, Abcam; and FOXA2: ab108422)

(4) Peptide-siRNA nanoparticles targeting NF-κB p50 mitigate experimental abdominal aortic aneurysm progression and ruptureBiomater AdvAugust 1, 2022Yan H, Hu Y, Akk A, Wickline SA, Pan H, Pham CTN.(1:200 dilution; Cat# ab33451, Abcam, Cambridge, MA), rabbit anti mouse pp65 (1:100 dilution; Cat# ab28856, Abcam, Cambridge, MA) or pp50 (1:100 dilution; Cat# NB100–82074, Novus Biologicals, Littleton, CO)

(5) Korean Red Ginseng Ameliorates Allergic Asthma through Reduction of Lung Inflammation and OxidationAntioxidants (Basel)July 22, 2022Jin-Hwa Kim, Jeong-Won Kim, Chang-Yeop Kim, Ji-Soo Jeong, Je-Oh Lim, Je-Won Ko, Tae-Won Kimagainst the following proteins: NF-κB (1:1000 dilution; ab16502; Abcam), phosphor-NF-κB (1:1000 dilution; ab28856; Abcam), iNOS (1:1000 dilution; ab15323; Abcam), HO-1 (1:1000; ab52947; Abcam), NRF2 (1:500 dilution;

(6) Renal denervation reduces atrial remodeling in hypertensive rats with metabolic syndromeBasic research in cardiologyJuly 14, 2022Selejan SR, Linz D, Mauz M, Hohl M, Huynh AKD, Speer T, Wintrich J, Kazakov A, Werner C, Mahfoud F, et al.against the extracellular domain of human RAGE (ab37647), HMGB1 (ab18256), CML (ab27684) and NFkB (ab28856 and ab131485) were from Abcam (Cambridge, UK), against collagen type I from Southern Biotech (1310-01,

(7) Empagliflozin mitigates type 2 diabetes-associated peripheral neuropathy: a glucose-independent effect through AMPK signalingArchives of pharmacal researchJuly 1, 2022Abdelkader NF, Elbaset MA, Moustafa PE, Ibrahim SM.polyclonal anti-RECK (Catalog No: ab88249), rabbit polyclonal anti-p(Ser536)-NF-κB p65 (Catalog No: ab28856) from Abcam (Waltham, MA, USA), besides rabbit polyclonal anti-p(Ser317)-ULK1 (Catalog No: 37762), rabbit

(8) The retinoid X receptor α modulator K-80003 suppresses inflammatory and catabolic responses in a rat model of osteoarthritisScientific ReportsDecember 1, 2021Hua Li, Xiaofan Li, Boyu Yang, Junnan Su, Shaofang Cai, Jinmei Huang, Tianfu Hu, Lijuan Chen, Yaping Xu, Yuhang Lidilution 1:1000); rabbit anti-rat ERα (Abcam, ab32063, dilution 1:1200); rabbit anti-rat p-p65 (Abcam, ab28856, dilution 1:2000); rabbit anti-rat p-IκBα (Abcam, ab32518, dilution 1:2,000); rabbit anti-rat HIF-2α

(9) MicroRNA‑3148 inhibits glioma by decreasing DCUN1D1 and inhibiting the NF‑kB pathwayExperimental and Therapeutic MedicineNovember 8, 2021Qianghua Xu, Xiao Chen, Bin Chenprotein (NF-κB) p50 antibody (cat. no. ab32360; 1:5,000; Abcam), anti-NF-κB p65 antibody (cat. no. ab28856; 1:1,000; Abcam)] and later with HRP-conjugated secondary antibodies (goat anti-rabbit, A0208; 1:1,000;

(10) miR‑142‑5p regulates lipopolysaccharide‑induced bovine epithelial cell proliferation and apoptosis via targeting BAG5Experimental and Therapeutic MedicineOctober 11, 2021Jinye Lu, Beibei Gu, Wei Lu, Jing Liu, Jiang Lu(1:1,000; cat. no. ab38449; Abcam), p65 (1:5,000; cat. no. ab32536; Abcam), p-p65 (1:1,000; cat. no. ab28856; Abcam), GAPDH (1:10,000; cat. no. ab181602; Abcam). Then the membranes were washed with PBS plus 0.1%

(11) Similarities and differences between IL11 and IL11RA1 knockout mice for lung fibro-inflammation, fertility and craniosynostosisScientific reportsJuly 8, 2021Ng B, Widjaja AA, Viswanathan S, Dong J, Chothani SP, Lim S, Shekeran SG, Tan J, McGregor NE, Walker EC, et al.Cell Signaling), anti-ERK1/2s (4695, Cell Signaling), anti-p-SMAD2 (ab216482, Abcam), anti-p-NF-kB (ab28856, Abcam), anti-p-STAT3 (4113, Cell Signaling), anti-SMAD2 (5339, Cell Signaling), anti-NF-kB (8242, Cell Signaling

(12) PARP inhibitors promote stromal fibroblast activation by enhancing CCL5 autocrine signaling in ovarian cancerNPJ precision oncologyJune 9, 2021Li X, Fang T, Xu S, Jin P, Zhou D, Wang Z, Li H, Yang Z, Chen G, Zheng X, et al.dilution), CCL5 (710001, Invitrogen, USA, used at 1/20 dilution), and phosphorylated (p)-NF-κB(Ser536) (ab28856, Abcam, USA, used at 1/100 dilution) overnight at 4 °C. The next day, the slides were incubated with

(13) LncRNA NEAT1 mediates intestinal inflammation by regulating TNFRSF1BAnnals of translational medicineMay 1, 2021Pan S, Liu R, Wu X, Ma K, Luo W, Nie K, Zhang C, Meng X, Tong T, Chen X, et al.(1:1,000; Cat. No. 10745-1-AP; Proteintech Group, Inc.), p-NF-κB p65 Rabbit pAb (1:1,000; cat. no. ab28856; Abcam, Inc.), β-actin Mouse mAb (1:2,000; cat. no. 66009-1-Ig; Proteintech Group, Inc.), IL1R1 Rabbit pAb

(14) Silencing cyclophilin A improves insulin secretion, reduces cell apoptosis, and alleviates inflammation as well as oxidant stress in high glucose-induced pancreatic β-cells via MAPK/NF-kb signaling pathwayBioengineeredSeptember 24, 2020Tangying Li, Huibiao Quan, Huachuan Zhang, Leweihua Lin, Qianying Ou, Kaining Chen(#ab32537, Abcam), P-ERK (#ab131438, Abcam), P38 (#ab170099, Abcam), P-P38 (#ab4822, Abcam), p-NF-KB P65 (#ab28856, Abcam), p-IKBα (#ab24783, Abcam), NF-KB P65 (#ab16502, Abcam), and IKBα (#ab32518, Abcam). Thereafter,

(15) Tumor Necrosis Factor Alpha Regulates Skeletal Myogenesis by Inhibiting SP1 Interaction with cis-Acting Regulatory Elements within the Fbxl2 Gene PromoterMolecular and cellular biologyMay 28, 2020O'Brien ME, Londino J, McGinnis M, Weathington N, Adair J, Suber T, Kagan V, Chen K, Zou C, Chen B, et al.obtained from Cell Signaling (Beverly, MA). NF-␬B (catalog no. ab16502) and phosphoNF-␬B p65 (catalog no. ab28856), and horseradish peroxidase (HRP)-tagged ␤-actin (catalog no. ab49900) antibodies were obtained from

(16) Nrf2 deficiency aggravates PM_2.5-induced cardiomyopathy by enhancing oxidative stress, fibrosis and inflammation via RIPK3-regulated mitochondrial disorderAgingMarch 17, 2020Ge C, Hu L, Lou D, Li Q, Feng J, Wu Y, Tan J, Xu M.light microscopy (Olympus, Tokyo, Japan). IHC analysis For immunostaining of 8-OHdG (ab48508, Abcam), pNF-κB (ab28856, Abcam) and RIPK3 (ab56164, Abcam), cardiac tissues were incubated in 3% H 2O2 for AGING 10 min

(17) Anti-VEGF therapy resistance in ovarian cancer is caused by GM-CSF-induced myeloid-derived suppressor cell recruitmentBritish Journal of CancerMarch 17, 2020Naoki Horikawa et al.Membranes were immunoblotted with the following antibodies: Rabbit anti-NF-κB p65 (phospho S536) antibody (ab28856, 1:500 dilution, Abcam), rabbit anti-NF-κB p65 antibody (#8242, 1:1000 dilution, Cell Signaling), rabbit

(18) Polyphyllin II inhibits liver cancer cell proliferation, migration and invasion through downregulated cofilin activity and the AKT/NF-κB pathwayBiology OpenFebruary 7, 2020Dejiang Pang, Chengcheng Yang, Chao Li, Yuanfeng Zou, Bin Feng, Lixia Li, Wentao Liu, Qihui Luo, Zhengli Chen, Chao Huangdilution), Lamin B1 (Cell Signaling Technology, 13435, 1:1000 dilution), p-NF-κB/P65 (S536) (Abcam, ab28856, 1:1000 dilution), Cofilin (Abcam, ab42824, 1:1000 dilution), pCofilin (Abcam, ab12866, 1:1000 dilution),

(19) Hesperetin reverses P‑glycoprotein‑mediated cisplatin resistance in DDP‑resistant human lung cancer cells via modulation of the nuclear factor‑κB signaling pathwayInternational Journal of Molecular MedicineFebruary 5, 2020Wencui Kong, Xiaoming Ling, Ying Chen, Xiaoli Wu, Zhongquan Zhao, Wenwu Wang, Shuiliang Wang, Guoxiang Lai, Zongyang Yuanti-NF-κB p65 (1:1,000; cat. no. ab16502; Abcam), rabbit anti-NF-κB p65 (p-S536) (1:1,000; cat. no. ab28856; Abcam), rabbit anti-histone H3 antibody (1:2,000; cat. no. ab201456; Abcam) or rabbit anti-human β-actin

(20) Fecal Microbiota Transplantation Prevents Intestinal Injury, Upregulation of Toll-Like Receptors, and 5-Fluorouracil/Oxaliplatin-Induced Toxicity in Colorectal CancerInternational Journal of Molecular SciencesJanuary 8, 2020Ching-Wei Chang et al.anti-Ki-67 (ab16667; Abcam, 1:200 dilution); anti-CD44 (ab157107; Abcam, 1:2000 dilution); anti-NF-κB (ab28856; Abcam, 1:50 dilution); and anti-ZO-1 (ab157107, 1:100 dilution). Negative control sections were processed

Write a Review

(1)

6/29/2015Detection of Phospho p65 using flow Rocky SateOur long term goal is to understand the process of tumorigenesis in colon cancer cells. In this experiment, we treated colon cancer cells with TNF-alpha to induce the p65 phosphorylation that was ... Read Review

Anti-NF-kB p65 (phospho S536) antibody from Abcam

Abcam

Citations (51)

Reviews

Anti-NF-kB p65 (phospho S536) antibody from Abcam

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map