COL1A1 Antibody PA5-29569 from Thermo Fisher Scientific

Product Specs
ItemCOL1A1 Antibody
CompanyThermo Fisher Scientific
Price Supplier Page View Company Product Page
Catalog NumberPA5-29569
Quantity100 µL
ApplicationsImmunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Conjugate/TagUnconjugated
FormatPBS with 20% glycerol and 0.025% ProClin 300; pH 7
ReactivityHuman, Mouse, Rat
ClonalityPolyclonal
TargetCOL1A1
IsotypeIgG
HostRabbit
NCBI Full Gene Namecollagen, type I, alpha 1, collagen type I alpha 1 chain
NCBI Gene AliasesCola-1, Cola1, Mov13, CAFYD, Col1a-1, EDSARTH1, Mov-13, EDSC, OI4, OI3, OI2, OI1, COLIA1
Concentration0.46 mg/mL
ImmunogenRecombinant protein fragment corresponding to a region within amino acids 261 and 525 of Human Transferrin
Storage Temperature-20° C, Avoid Freeze/Thaw Cycles
PurityAntigen affinity chromatography
Antibody TypePrimary
StorageStore at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

Company Profile

Website: www.thermofisher.com

(1) Lobeglitazone inhibits LPS-induced NLRP3 inflammasome activation and inflammation in the liverPLoS OneAugust 24, 2023Hye-Young Seo, So-Hee Lee, Ji Yeon Park, Eugene Han, Sol Han, Jae Seok Hwang, Mi Kyung Kim, Byoung Kuk JangAn anti-IL1β antibody (ab9722) was purchased from Abcam (Cambridge, UK). An anticollagen antibody (PA529569) was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Anti-NLRP3 (CS1510), anticleaved caspase 1

(2) Cationic proteins from eosinophils bind bone morphogenetic protein receptors promoting vascular calcification and atherogenesisEuropean heart journalAugust 1, 2023Meng Z, Zhang S, Li W, Wang Y, Wang M, Liu X, Liu CL, Liao S, Liu T, Yang C, et al.R&D Systems), mouse anti-BMPR-2 (1:1000, MA5-15827, Thermo Fisher Scientific), mouse anti-collagen I (1:1000, PA5-29569, Thermo Fisher Scientific), rabbit anti-osteocalcin (1:1000, AB10911, Sigma-Aldrich), mouse anti-osteopontin

(3) Perfusion and Ultrasonication Produce a Decellularized Porcine Whole-Ovary Scaffold with a Preserved MicroarchitectureCellsJuly 15, 2023Gustavo Henrique Doná Rodrigues Almeida, Leandro Norberto da Silva-Júnior, Mariana Sversut Gibin, Henrique dos Santos, Bianca de Oliveira Horvath-Pereira, Leticia Beatriz Mazo Pinho, Mauro Luciano Baesso, Francielle Sato, Luzmarina Hernandes, Charles R. Long, et al.for non-specific protein interaction blockage. The primary antibodies used were: anti-collagen I (#PA5-29569, 1:250, Invitrogen, Carlsbad, CA, USA), anti-collagen III (#PA1-28870, 1:250; Invitrogen), anti-fibronectin

(4) Peelable Microneedle Patches Deliver Fibroblast Growth Factors to Repair Skin Photoaging DamageNanotheranosticsJune 19, 2023Guojun Yang, Shiqi Hu, Haiyue Jiang, Ke ChengGermany). The antibodies used were as follows: COL1A1 polyclonal antibody (COL1A1 antibody (diluted at 1/100; PA5-29569; Invitrogen), and elastin polyclonal antibody (diluted at 1/200; ab 21610; Abcam, Cambridge, UK). Cytokine

(5) Kaempferide Prevents Photoaging of Ultraviolet-B Irradiated NIH-3T3 Cells and Mouse Skin via Regulating the Reactive Oxygen Species-Mediated SignalingsAntioxidants (Basel, Switzerland)December 21, 2022Choi JK, Kwon OY, Lee SH.

(6) Enhanced Expression of ARK5 in Hepatic Stellate Cell and Hepatocyte Synergistically Promote Liver FibrosisInternational Journal of Molecular SciencesOctober 28, 2022Yang You, Chongqing Gao, Junru Wu, Hengdong Qu, Yang Xiao, Ziwei Kang, Jinying Li, Jian Honganti-α-SMA (Invitrogen, Waltham, MA, USA, PA5-18292), anti-collagen I (Invitrogen, Waltham, MA, USA, PA5-29569) primary antibodies overnight at 4 °C. Positive staining was visualized with the EnVision immunodetection

(7) Impaired non-canonical transforming growth factor-β signalling prevents profibrotic phenotypes in cultured peptidylarginine deiminase 4-deficient murine cardiac fibroblastsJournal of cellular and molecular medicineOctober 1, 2021Akboua H, Eghbalzadeh K, Keser U, Wahlers T, Paunel-Görgülü A.blocking, membranes were incubated with the following primary antibodies overnight at 4°C: Collagen I (PA5‐29569, Thermo Scientific, 1:1000), Collagen III (PA5‐27828, Thermo Scientific, 1:1000), α‐SMA (14968, Cell Signaling

(8) Simvastatin modulates estrogen signaling in uterine leiomyoma via regulating receptor palmitoylation, trafficking and degradationPharmacological ResearchOctober 1, 2021Sadia Afrin et al.#4370), pAKT (Santa Cruz; sc-7985), AKT (Santa Cruz; sc-8312), anti-collagen 1 (Thermo Fisher Scientific, PA5–29569), anti-β-actin (Sigma, A3854), anti-Na,K-ATPase (CST, 23565), or anti-lamin B1 (Invitrogen, PA5–19468)

(9) Human Mesenchymal Stromal Cell-Derived Exosomes Promote In Vitro Wound Healing by Modulating the Biological Properties of Skin Keratinocytes and Fibroblasts and Stimulating AngiogenesisInternational journal of molecular sciencesJune 9, 2021Tutuianu R, Rosca AM, Iacomi DM, Simionescu M, Titorencu I.h at room temperature, after which they were incubated with primary antibodies to type I collagen (PA5-29569, ThermoFisher Scientific), fibronectin (PA5-29578, ThermoFisher Scientific), αSMA (14-9760-82, Invitrogen,

(10) Translation of Tudor-SN, a novel terminal oligo-pyrimidine (TOP) mRNA, is regulated by the mTORC1 pathway in cardiomyocytesRNA biologyJune 1, 2021Gan S, Su C, Ma J, Liu M, Cui X, Xin L, Ren Y, Gao X, Ge L, Wei M, et al.

(11) Dystrophin Deficiency Causes Progressive Depletion of Cardiovascular Progenitor Cells in the HeartInternational journal of molecular sciencesMay 10, 2021Jelinkova S, Sleiman Y, Fojtík P, Aimond F, Finan A, Hugon G, Scheuermann V, Beckerová D, Cazorla O, Vincenti M, et al.membranes were incubated with primary antibodies against collagen 1A1 (COL1A1, rabbit polyclonal, ref PA5-29569, Thermo Fisher), collagen 3 (rabbit polyclonal, ref PA5-34787, Thermo Fisher), mouse matrix metalloproteinase

(12) Deficient Endoplasmic Reticulum Acetyl-CoA Import in Pancreatic Acinar Cells Leads to Chronic PancreatitisCellular and molecular gastroenterology and hepatologyJanuary 1, 2021Cooley MM, Thomas DDH, Deans K, Peng Y, Lugea A, Pandol SJ, Puglielli L, Groblewski GE.MA)",PA5-16385 CD3,AbCam,Ab16669 CD45R,AbCam,Ab64100 CHOP,"Cell Signaling (Danvers, MA)",cs-2895 "Collagen, type 1",Thermo Fisher,PA5-29569 eIF2α,Cell Signaling,cs-9722 p-eIF2α,Cell Signaling,cs-9721 F4/80,AbCam,Ab6640 GADD34,Santa Cruz,sc-8327 JNK,Cell Signaling

(13) An Injectable Fibrin Scaffold Rich in Growth Factors for Skin RepairBioMed research internationalJanuary 1, 2021Shao Z, Lyu C, Teng L, Xie X, Sun J, Zou D, Lu J.10% normal serum at 37°C for 30 min, and then incubated with primary antibodies against collagen I (PA5-29569, Thermo Fisher Scientific, USA) and VEGF (MA1-16629, Thermo Fisher Scientific, USA) at 4°C overnight.

(14) WSB2 as a target of Hedgehog signaling promoted the malignant biological behavior of Xuanwei lung cancer through regulating Wnt/β-catenin signalingTranslational Cancer ResearchDecember 1, 2020Xueqiang Wei, Jun Liao, Yujie Lei, Minjie Li, Guangqiang Zhao, Yongchun Zhou, Lianhua Ye, Yunchao Huangthe primary antibodies against WSB2 (ab127176, Abc4am, USA), E-cadherin (PA512542, Pierce, USA), N-cadherin (PA529569, Pierce), vimentin (PA512321, Pierce), c-Myc (ab39688, Abcam), β-catenin (DXT-130-107-174, Miltenyi, USA)

(15) Effect of Lactobacillus reuteri Administration on Wrinkle Formation and Type I Procollagen Levels in UVB-Exposed Male Balb/c Mice (Mus musculus)Molecular and Cellular Biomedical SciencesNovember 2, 2020Ivanna Valentina, Achadiyani Achadiyani, Sunarjati Sudigdo Adi, Ronny Lesmana, Reni FareniaMembrane was then incubated overnight at 4oC using the anti-procollagen I (Catalogue Group 0 Group 1 #PA5-29569, Thermo Scientific, Massachusetts, USA) and anti-β-actin antibody (Catalogue #MA5-15739, Thermo Scientific)

(16) Simvastatin ameliorates altered mechanotransduction in uterine leiomyoma cellsAmerican Journal of Obstetrics and GynecologyNovember 1, 2020Sadia Afrin, Md Soriful Islam, Kristin Patzkowsky, Minnie Malik, William H. Catherino, James H. Segars, Mostafa A. BorahayMembrane were incubated with specific primary antibodies: anti-collagen I (Thermo Fisher Scientific, #PA5-29569), anti-β1 integrin (Cell Signaling Technology (CST), #4706), anti-FAK (CST, #3285S), anti-phospho-FAK

(17) Small molecule inhibitor of HSP47 prevents pro-fibrotic mechanisms of fibroblasts in vitroBiochemical and biophysical research communicationsSeptember 24, 2020Miyamura T, Sakamoto N, Kakugawa T, Taniguchi H, Akiyama Y, Okuno D, Moriyama S, Hara A, Kido T, Ishimoto H, et al.Thermo Fisher Scientific) diluted in TBS-T (1:1,000 dilution) or an anti-collagen I polyclonal antibody (PA5–29569, Thermo Fisher Scientific) diluted in TBS-T (1:5,000 dilution) overnight at 4 °C after 1 h at room temperature.

(18) Phagocytosis of Wnt inhibitor SFRP4 by late wound macrophages drives chronic Wnt activity for fibrotic skin healingScience advancesMarch 1, 2020Gay D, Ghinatti G, Guerrero-Juarez CF, Ferrer RA, Ferri F, Lim CH, Murakami S, Gault N, Barroca V, Rombeau I, et al.Thermo Fisher Scientific), EDA-FN clone IST-9 (Abcam ab6328), and anti-FN biotin (Abcam ab6584); Cola1a: Rabbit polyclonal (PA5-29569, Thermo Fisher Scientific). Anti–β-Cat (Abcam ab6302) and anti–β-GAL (Abcam ab9361) were used for Wnt

(19) Mast cell-deficiency protects mice from streptozotocin-induced diabetic cardiomyopathyTranslational ResearchJune 1, 2019Aina He, Wenqian Fang, Kun Zhao, Yajun Wang, Jie Li, Chongzhe Yang, Feriel Benadjaoud, Guo-Ping ShiCell Signaling Technology), α-SMA (1:1000, Cat#14968s, Cell Signaling Technology), collagen-I (1:1000, Cat#PA5–29569, Thermo Fisher Scientific), collagen-III (1:1000, Cat#PA5–27828, Thermo Fisher Scientific), and β-actin

(20) FOXM1 is a critical driver of lung fibroblast activation and fibrogenesisThe Journal of clinical investigationJune 1, 2018Penke LR, Speth JM, Dommeti VL, White ES, Bergin IL, Peters-Golden M.pFOXO3A (catalog ab154786), and BIRC5 (catalog ab76424) purchased from Abcam; collagen 1A1 (catalog PA5-29569) purchased from Thermo Scientific; and α-tubulin (catalog T9026; clone DM1A) purchased from Sigma-Aldrich.

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COL1A1 Antibody from Thermo Fisher Scientific

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COL1A1 Antibody from Thermo Fisher Scientific

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