Anti-TPX2 antibody [18D5-1] ab32795 from Abcam

Product Specs
ItemAnti-TPX2 antibody [18D5-1]
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab32795
Domain/Region/Terminus5-1
Antibody TypePrimary
Quantity100 UG
ApplicationsImmunocytochemistry, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Clone18D5-1
ClonalityMonoclonal
IsotypeIgG
HostMouse, Nude mouse
Conjugate/TagUnconjugated
Concentration1 mg/ml
Gene NameTPX2
ReactivityHuman, Mouse
TargetTPX2

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) PP6 regulation of Aurora A-TPX2 limits NDC80 phosphorylation and mitotic spindle sizeThe Journal of cell biologyMay 1, 2023Sobajima T, Kowalczyk KM, Skylakakis S, Hayward D, Fulcher LJ, Neary C, Batley C, Kurlekar S, Roberts E, Gruneberg U, et al.[NUPs]; mouse mAb, ab24609; Abcam), NUP153 (rabbit pAb, A301-788A; Bethyl Laboratories), TPX2 (mouse mAb, ab32795; Abcam), MAD1 (rabbit pAb, GTX105079; GeneTex), pericentrin (rabbit pAb, ab4448; Abcam), pT210 PLK1

(2) LncRNA KCNQ1OT1 enhances the radioresistance of lung squamous cell carcinoma by targeting the miR-491-5p/TPX2-RNF2 axisJournal of thoracic diseaseOctober 1, 2022Liu J, Jiang M, Guan J, Wang Y, Yu W, Hu Y, Zhang X, Yang J.cat. no. 9664, Cell Signaling Technology), Xenopus kinesin-like protein 2 (TPX2; 1:1,000, cat. no. ab32795, Abcam), RING finger protein 2 (RNF2; 1:1,000, cat. no. ab101273, Abcam), and β-actin (1:5,000, cat.

(3) TPX2 is a prognostic marker and promotes cell proliferation in neuroblastomaOncology LettersFebruary 28, 2022Yuhki Koike et al.Immunohistochemical staining Immunohistochemical staining of NB tissues was performed using an anti-human TPX2 antibody (1:1,000; cat. no. ab32795; Abcam) as previously described (22). Western blotting At 48 h after transfection, cells were lysed

(4) The Aurora-A/TPX2 Axis Directs Spindle Orientation in Adherent Human Cells by Regulating NuMA and Microtubule StabilityCurrent biology : CBFebruary 8, 2021Polverino F, Naso FD, Asteriti IA, Palmerini V, Singh D, Valente D, Bird AW, Rosa A, Mapelli M, Guarguaglini G.AB_10863599 rabbit anti-pericentrin Abcam Cat#Ab4448; RRID: AB_304461 mouse anti-TPX2 Abcam Cat#Ab32795; RRID: AB_778561 rabbit anti-TPX2 Novus Biologicals Cat#NB500-179; RRID: AB_527246 rabbit anti-TPX2 Made

(5) Ordered dephosphorylation initiated by the selective proteolysis of cyclin B drives mitotic exiteLifeSeptember 1, 2020Holder J, Mohammed S, Barr FA.monoclonal),AbCam,ID_source:EPR3240; ab79546; RRID:AB_2173411,WB (1:1000) Antibody,Anti-TPX2 (mouse monoclonal),AbCam,ID_source:ab32795; RRID:AB_778561,WB (1:2000) Antibody,Anti-tubulin (mouse monoclonal),Sigma,ID_source:T6199; Clone DM1A;

(6) Excess TPX2 Interferes with Microtubule Disassembly and Nuclei Reformation at Mitotic ExitCellsFebruary 6, 2020Naso FD, Sterbini V, Crecca E, Asteriti IA, Russo AD, Giubettini M, Cundari E, Lindon C, Rosa A, Guarguaglini G.B1 (Abcam, ab16048, 1 µg/mL), rabbit anti-pericentrin (Abcam, ab4448, 1 µg/mL), mouse anti-TPX2 (Abcam, ab32795, 4 µg/mL). Samples were analysed using a Nikon Eclipse 90i microscope (Nikon Instruments S.p.A., Campi

(7) Tumor-Suppressive MicroRNA-216b Binds to TPX2, Activating the p53 Signaling in Human Cutaneous Squamous Cell CarcinomaMolecular Therapy. Nucleic AcidsJanuary 28, 2020Cheng Feng, Hai-Lin Zhang, Ang Zeng, Ming Bai, Xiao-Jun Wangsolution and allowed to stand at 37°C for 30 min. Then, mouse anti-human antibody to TPX2 (1:1,000, ab32795, Abcam, Cambridge, MA, USA) was added as primary antibody and mouse anti-human antibody to immunoglobulin

(8) TPX2 silencing exerts anti‑tumor effects on hepatocellular carcinoma by regulating the PI3K/AKT signaling pathwayInternational journal of molecular medicineDecember 1, 2019Huang DH, Jian J, Li S, Zhang Y, Liu LZ.9145) and β ‑actin (cat. no. 4970) were purchased from Cell Signaling Technology. Anti‑TPX2 (cat. no. ab32795) primary antibody for immunohistochemical staining was purchased from Abcam. Primary antibodies against

(9) A molecular and staging model predicts survival in patients with resected non-small cell lung cancerBMC CancerDecember 1, 2018Lei Liu, Minxin Shi, Zhiwei Wang, Haimin Lu, Chang Li, Yu Tao, Xiaoyan Chen, Jun Zhaocitrate buffer (pH 6.0). Sections were incubated overnight at 4 °C with mouse anti-TPX2 (1:200 dilution, ab32795, Abcam, Cambridge, UK) and rabbit anti-MMP12 (1:50 dilution, SC-30072, Santa Cruz, Texas, USA) antibodies. Then,

(10) Adducin-1 is essential for spindle pole integrity through its interaction with TPX2EMBO reportsAugust 1, 2018Hsu WH, Wang WJ, Lin WY, Huang YM, Lai CC, Liao JC, Chen HC.(ab39068), anti‐Aurora‐A (ab13824), anti‐NEDD1 (ab57336), mouse anti‐pericentrin (ab28144), anti‐TPX2 (ab32795), and anti‐separase (ab16170) antibodies were purchased from Abcam. The rabbit monoclonal anti‐Aurora‐A

(11) Role of PLK1 signaling pathway genes in gastrointestinal stromal tumorsOncology LettersJune 21, 2018Jen‑Shi Chen et al.benzimidazoles (BUB1; catalog no. ab4636; 1:100), and anti-targeting protein for Xklp2 (TPX2; catalog no. ab32795; 1:1,000) antibodies from Abcam, (Cambridge, UK). The antibodies were diluted as suggested and added

(12) Allosteric modulation of AURKA kinase activity by a small-molecule inhibitor of its protein-protein interaction with TPX2Scientific ReportsSeptember 1, 2016Matej Janeček et al.

(13) The deubiquitinating enzyme complex BRISC is required for proper mitotic spindle assembly in mammalian cellsThe Journal of cell biologyJuly 20, 2015Yan K, Li L, Wang X, Hong R, Zhang Y, Yang H, Lin M, Zhang S, He Q, Zheng D, et al.antibodies were used: rabbit anti-NuMA (ab36999; Abcam; NB100-74636; Novus Biologicals); mouse anti-TPX2 (ab32795; Abcam); rabbit anti–α tubulin (DM1A; Cell Signaling Technology); rabbit anti–importin-β (no. 8673S;

(14) Mio depletion links mTOR regulation to Aurora A and Plk1 activation at mitotic centrosomesThe Journal of cell biologyJuly 6, 2015Platani M, Trinkle-Mulcahy L, Porter M, Jeyaprakash AA, Earnshaw WC.

(15) Positive Surgical Margin, HPV Persistence, and Expression of Both TPX2 and PD-L1 Are Associated with Persistence/Recurrence of Cervical Intraepithelial Neoplasia after Cervical ConizationPloS oneJanuary 1, 2015Hui Zhang, Tingguo Zhang, Zongbing You, Youzhong Zhang

(16) Melanoma-associated mutations in protein phosphatase 6 cause chromosome instability and DNA damage owing to dysregulated Aurora-AJournal of cell scienceAugust 1, 2013Dean Hammond, Kang Zeng, Antonio Espert, Ricardo Nunes Bastos, Ryan D Baron, Ulrike Gruneberg, Francis A Barr

(17) Aurora kinase inhibitors reveal mechanisms of HURP in nucleation of centrosomal and kinetochore microtubulesProceedings of the National Academy of Sciences of the United States of AmericaMay 7, 2013Wu JM, Chen CT, Coumar MS, Lin WH, Chen ZJ, Hsu JT, Peng YH, Shiao HY, Lin WH, Chu CY, et al.were obtained from the following sources. Abcam: mouse anti–γ-tubulin (ab11316) and mouse anti-TPX2 (ab32795); Sigma-Aldrich: mouse anti–α-tubulin (T5168) and mouse anti-actin (A1978); Santa Cruz Biotechnology:

(18) APC(FZR1) prevents nondisjunction in mouse oocytes by controlling meiotic spindle assembly timingMolecular biology of the cellOctober 1, 2012Holt JE, Lane SI, Jennings P, García-Higuera I, Moreno S, Jones KT.(ab72; Abcam), securin (ab3305; Abcam), CDC20 (sc8358; Santa Cruz Biotechnology, Santa Cruz, CA), TPX2 (ab32795; Abcam), Hurp (sc98809; Santa Cruz Biotechnology), Eg5 (NB100-8467; Novus Biologicals, Littleton, CO),

(19) Ashwagandha Derived Withanone Targets TPX2-Aurora A Complex: Computational and Experimental Evidence to its Anticancer ActivityPLoS ONEJanuary 27, 2012Abhinav Grover, Rumani Singh, Ashutosh Shandilya, Didik Priyandoko, Vibhuti Agrawal, Virendra S. Bisaria, Renu Wadhwa, Sunil C. Kaul, Durai Sundar

(20) Protein phosphatase 6 regulates mitotic spindle formation by controlling the T-loop phosphorylation state of Aurora A bound to its activator TPX2The Journal of cell biologyDecember 27, 2010Zeng K, Bastos RN, Barr FA, Gruneberg U.Bethyl Laboratories, Inc.), PP1-γ (goat SC-6108; Santa Cruz Biotechnology, Inc.), PP2A (mouse 610555; BD), TPX2 (mouse ab32795; Abcam), Hec1 (mouse GTX70268; GeneTex, Inc.), CenpE (mouse ab5093; Abcam), CenpA (mouse ab13939; Abcam),

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4/8/2016Nice Anti-TPX2 Antibody Rebecca WatesThe focus of this work was to characterize expression of mitosis-related proteins in HeLa cells. This is a monoclonal antibody from Abcam, that has been refined to ensure high specificity.

Anti-TPX2 antibody [18D5-1] from Abcam

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Anti-TPX2 antibody [18D5-1] from Abcam

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