Anti-Integrin beta 1 antibody [12G10] ab30394 from Abcam

Product Specs
ItemAnti-Integrin beta 1 antibody [12G10]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab30394
Antibody TypePrimary
Quantity10 UG, 100 UG
ApplicationsELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western blot
IsotypeIgG1
ClonalityMonoclonal
HostMouse, Nude mouse
Clone12G10
Conjugate/TagUnconjugated
FormatPurified from tissue culture supernatant
Concentration1 mg/ml
Gene NameITGB1
ValidationKnockOut Validated
ReactivityHuman
TargetIntegrin beta 1

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Abcam

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United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
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+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) PFKFB3 downregulation aggravates Angiotensin II-induced podocyte detachmentRenal FailureDecember 31, 2023Xiaoxiao Huang, Zhaowei Chen, Zilv Luo, Yiqun Hao, Jun Feng, Zijing Zhu, Xueyan Yang, Zongwei Zhang, Jijia Hu, Wei Liang, et al.detect their respective antigens: anti-PFKFB3 (ab181861; Abcam Plc., UK), anti-integrin beta1 (12G10) (ab30394; Abcam Plc., UK), anti-talin1 (YP1523; Immunoway Biotechnology Co., Plano, TX, USA), anti-GAPDH (600004-1;

(2) Abnormal basement membrane results in increased keratinocyte-derived periostin expression in psoriasis similar to wound healingScientific ReportsSeptember 29, 2023Lili Borbála Flink, Ameneh Ghaffarinia, Benjamin Tamás Papp, Ákos Varga, András István Vigh, Dániel László Vidács, Róbert Kui, Lajos Kemény, Zsuzsanna Bata-Csörgő, Renáta Bozómouse anti-human periostin (1:125, #sc‐398631, Santa Cruz Biotechnology), and β1-integrin (1:100, #ab30394, Abcam, Cambridge, UK) were used overnight followed by Alexa Fluor 647 conjugated goat anti-mouse IgG

(3) RTK signalling promotes epithelial columnar cell shape and apical junction maintenance in human lung progenitor cellsDevelopmentJune 1, 2023Shuyu Liu, Dawei Sun, Richard Butler, Emma L. Rawlinsng/ml recombinant rabbit integrin α2 (Abcam, ab181548) and 10 ng/ml mouse IgG1 integrin β1 (Abcam, ab30394) or 10 ng/ml mouse monoclonal 2C11 IgG1 isotype control antibody (Abcam, ab1927) for 6 days with culture

(4) Extended preconditioning on soft matrices directs human mesenchymal stem cell fate via YAP transcriptional activity and chromatin organizationAPL BioengineeringMarch 1, 2023Yufei Ma, Xu Zhang, Shaoxin Tang, Li Xue, Jing Wang, Xiaohui Zhangwith 5% BSA for 30 min. Then, cells were incubated with anti-Integrin beta 1 antibody (1:200, Abcam, ab30394) at 4 °C overnight. Goat anti-mouse IgG (Alexa Fluor® 488) (1:1000, Abcam, ab150113) was used as secondary

(5) Activation of autophagy triggers mitochondrial loss and changes acetylation profile relevant for mechanotransduction in bladder cancer cellsArchives of ToxicologyJanuary 1, 2023Maximilian Jobst, Endre Kiss, Christopher Gerner, Doris Marko, Giorgia Del FaveroAntibody (1C6) (MA1-2021, Invitrogen), anti-Caveolin-1 (ab192452, Abcam, dil 1:500), anti-Integrin beta 1 (ab30394, Abcam, dil 1:500), anti-PMP70 (PA1-650, Invitrogen dil 1:500), anti-CLUH (PA5-65,101, Invitrogen, dil

(6) CSP I-plus modified rEndostatin inhibits hepatocellular carcinoma metastasis via down-regulation of VEGFA and integrinβ1BMC CancerNovember 22, 2022Xueqin Chen, Yan Wang, Hancong Liu, Jingjing Zhang, Jie Wang, Xiaobao Jin, Yan Mathen probed with respective primary antibodies against VEGFA (ab52917), MMP2 (ab37150), integrinβ1 (ab30394), and E-cadherin (ab76055) procured from Abcam, β-actin (AA128) from Beyotime Biotechnology, Shanghai,

(7) Programmable integrin and N-cadherin adhesive interactions modulate mechanosensing of mesenchymal stem cells by cofilin phosphorylationNat CommunNovember 11, 2022Zheng Zhang, Baoyong Sha, Lingzhu Zhao, Huan Zhang, Jinteng Feng, Cheng Zhang, Lin Sun, Meiqing Luo, Bin Gao, Hui Guo, et al.Primary antibodies and dilutions used in this study contained anti-Integrin β1 (1:500, mouse, Abcam, ab30394), anti-N-Cadherin (1:200, rabbit, Cell Signaling, 13116), anti-YAP (1:100, rabbit, Cell Signaling, 14074),

(8) CD82 attenuates TGF-Î²1-mediated epithelial-mesenchymal transition by blocking smad-dependent signaling in ARPE-19 cellsFrontiers in PharmacologyOctober 25, 2022Hyesook Lee, Jung-Hwa Han, Yun Jeong Kang, Hyun Hwangbo, Aeseon Yoon, Hyung-Sik Kim, Dongjun Lee, Soo Yong Lee, Byung Hyun Choi, Jae-Joon Kim, et al.No. 53-9760-82, Thermo Fisher Scientific, Waltham, MA, United States), anti-integrin β1 (catalog No. ab30394, Abcam Inc., Cambridge, United Kingdom), anti-integrin αvβ5 (catalog No. ab177004, Abcam Inc.), anti-TGFβ

(9) Mechanical Confinement and DDR1 Signaling Synergize to Regulate Collagenâ€Induced Apoptosis in Rhabdomyosarcoma CellsAdvanced ScienceOctober 1, 2022Jordi Gonzalezâ€Molina, Katharina Miria Kirchhof, Bhavik Rathod, Lidia Moyanoâ€Galceran, Maria Calvoâ€Noriega, Georgia Kokaraki, Astrid BjÃ¸rkÃ¸y, Monika Ehnman, Joseph W. Carlson, Kaisa Lehtiimmunofluorescence were Ki67 (8D5; #9449; Cell Signaling Technology; 1:800), active integrin β1 (12G10; ab30394; Abcam; 1:400), YAP/TAZ (63.7; sc‐101199; Santa Cruz Biotechnology; 1:200), cleaved‐caspase 3 (Asp175;

(10) Control cell migration by engineering integrin ligand assemblyNature CommunicationsAugust 25, 2022Xunwu Hu et al.they were probed with antibodies against GAPDH (6C5, ab8245, Abcam, 1:1000), integrin beta 1 (12G10, ab30394, Abcam, 1:100), integrin alpha 3 (ASC-1, MA5-28565, Invitrogen, 1:100), integrin alpha 6 (EPR18124,

(11) BMSC-EV-derived lncRNA NORAD Facilitates Migration, Invasion, and Angiogenesis in Osteosarcoma Cells by Regulating CREBBP via Delivery of miR-877-3pOxidative Medicine and Cellular LongevityMarch 1, 2022Dapeng Feng, Zhengwei Li, Liang Yang, Haidong Liang, Hongtao He, Lin Liu, Wei ZhangAlcian Blue (G2542, Solarbio), respectively. The expressions of BMSC surface markers CD29 (1 : 1,000, ab30394, Abcam, Cambridge, MA, USA), CD90 (1 : 1,000, ab25672), CD34 (1 : 1,000, ab81289), and CD45 (1 : 1,000,

(12) Toxoplasma gondii SAG1 targeting host cell S100A6 for parasite invasion and host immunityiScienceDecember 17, 2021Zhou LJ, Peng J, Chen M, Yao LJ, Zou WH, He CY, Peng HJ.antibody,Abcam,RRID: AB_306466 Anti-DPP4,Abcam,Cat#ab114033 Anti-DPP4,Abcam,RRID: AB_10901695 Anti-ITGβ1,Abcam,Cat#ab30394 Anti-ITGβ1,Abcam,RRID: AB_775726 Anti-MYH9,Abcam,Cat#ab24762 Anti-MYH9,Abcam,RRID: AB_2282292 Anti-CKAP4,Thermo Fisher Scientific

(13) A proximity proteomics screen in three-dimensional spheroid cultures identifies novel regulators of lumen formationScientific reportsNovember 23, 2021Wang LT, Proulx MÈ, Kim AD, Lelarge V, McCaffrey L.1/200 (Cell Signaling #3195S), V5 1/200 (Thermo Fisher Scientific # R960-25), β1-integrin 1/200 (Abcam #ab30394), ZO-1 1/100 (Cell Signaling #8193S), YAP 1/100 (Cell Signaling #14074) and Phalloidin 1/100 (Invitrogen

(14) Oncogenic RAS instructs morphological transformation of human epithelia via differential tissue mechanicsScience AdvancesOctober 15, 2021Agata Nyga, Jose J. Muñoz, Suze Dercksen, Giulia Fornabaio, Marina Uroz, Xavier Trepat, Buzz Baum, Helen K. Matthews, Vito ContePrimary antibodies (mouse E-cadherin, 1:1500; BD Biosciences, #610181; mouse integrin β1, 1:250; Abcam, ab30394; rabbit pMLC2, 1:50; Cell Signaling Technology, #3671S; mouse integrin β3, 1:200; Merck, #MAB2023Z;

(15) CYRI-A limits invasive migration through macropinosome formation and integrin uptake regulationThe Journal of cell biologySeptember 6, 2021Le AH, Yelland T, Paul NR, Fort L, Nikolaou S, Ismail S, Machesky LM.Western blotting), Phalloidin 488 (#A12379; Molecular Probes), anti-Integrin β 1 antibody [12G10] (rabbit, #ab30394, RRID:AB_775726; Abcam; immunofluorescent and Western blotting), recombinant anti-Integrin α 5 antibody

(16) A stem cell-derived ovarian regenerative patch restores ovarian function and rescues fertility in rats with primary ovarian insufficiencyTheranosticsAugust 18, 2021Sichen Zhang et al.Coulter flow cytometer (Brea, CA). Huc-MSCs were characterized by primary antibodies, including CD29 (ab30394), CD31 (ab119339), CD105 (ab107595), CD34 (ab81289), CD90 (ab226), and CD45 (ab10558). All antibodies

(17) CD13 orients the apical-basal polarity axis necessary for lumen formationNature communicationsAugust 4, 2021Wang LT, Rajah A, Brown CM, McCaffrey L.1/200 (Cell Signaling #3145), Pals1 1/100 (proteintech group #17710-1-AP), β1-integrin 1/200 (Abcam #ab30394), V5 1/200 (Thermo Fisher Scientific # R960-25), laminin 1/200 (Abcam #ab11575), α-Tubulin 1/200 (Sigma

(18) Targeting novel LSD1-dependent ACE2 demethylation domains inhibits SARS-CoV-2 replicationCell discoveryMay 24, 2021Tu WJ, McCuaig RD, Melino M, Rawle DJ, Le TT, Yan K, Suhrbier A, Johnston RL, Koufariotis LT, Waddell N, et al.polyclonal anti-KDM1/LSD1 (Abcam, ab17721) overnight at 4 °C. Mouse monoclonal anti-integrin beta I (Abcam, ab30394) was used as a positive control for cell membrane protein extraction. Primary antibodies were detected

(19) Neutrophils interact with cholangiocytes to cause cholestatic changes in alcoholic hepatitisGutFebruary 1, 2021Takeuchi M, Vidigal PT, Guerra MT, Hundt MA, Robert ME, Olave-Martinez M, Aoki S, Khamphaya T, Kersten R, Kruglov E, et al.Laboratories), NFκB-p65 (Clone E379, ab32536, Abcam), RAC1 (PA1-091, Invitrogen), ITGB1 (Clone 12G10, ab30394, Abcam), JNK (9252, Cell Signaling Technology), phospho-JNK (pJNK, 9251, Cell Signaling Technology)

(20) Cx43 Present at the Leading Edge Membrane Governs Promigratory Effects of Osteoblast-Conditioned Medium on Human Prostate Cancer Cells in the Context of Bone MetastasisCancers (Basel)October 16, 2020Jonathan Boucher et al.supplied by Sigma (St. Louis, MO, USA). Mouse monoclonal antibody anti-β1Integrin was from Abcam (#ab30394, Cambridge, England). Mouse monoclonal antibody directed against Cx43 C-ter was purchased from BD Transduction

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Anti-Integrin beta 1 antibody [12G10] from Abcam

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Anti-Integrin beta 1 antibody [12G10] from Abcam

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