Anti-EpCAM antibody [E144] ab32392 from Abcam

Product Specs
ItemAnti-EpCAM antibody [E144]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab32392
Antibody TypePrimary
HostRabbit
IsotypeIgG
ApplicationsWestern blot
ClonalityMonoclonal
CloneE144
Quantity10 UL, 40 UL, 100 UL
Conjugate/TagUnconjugated
Research AreaImmuno-oncology
Gene NameEPCAM
ValidationKnockOut Validated
ReactivityHuman, Mouse, Rat
TargetEpCAM

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Obesity impairs cardiolipin-dependent mitophagy and therapeutic intercellular mitochondrial transfer ability of mesenchymal stem cellsCell death & diseaseMay 13, 2023Sagar S, Faizan MI, Chaudhary N, Singh V, Singh P, Gheware A, Sharma K, Azmi I, Singh VP, Kharya G, et al.IV,Abcam,ab14744 13,Anti-FUNDC1,Abcam,ab74834 14,Anti-DRP1,Abcam,ab140494 15,Anti-SQSTM1/p62,Abcam,ab56416 16,Anti-Uteroglobin (CCSP),Abcam,ab40873 17,Recombinant Anti-EpCAM,Abcam,ab32392 18,Anti-alpha Tubulin,Abcam,ab7291 19,Anti-Mitofusin 1,Abcam,ab57602 20,Anti-LAMP1,Abcam,ab24170 21,Anti-Cytochrome

(2) CD26-negative and CD26-positive tissue-resident fibroblasts contribute to functionally distinct CAF subpopulations in breast cancerNature communicationsJanuary 12, 2023Houthuijzen JM, de Bruijn R, van der Burg E, Drenth AP, Wientjens E, Filipovic T, Bullock E, Brambillasca CS, Pulver EM, Nieuwland M, et al.(Cell signaling, #3196), SMA (Thermo Scientific, RB-9010), vimentin (Cell signaling, #5741), EpCAM (Abcam, ab32392), E-cadherin (Cell signaling, #3195), rat-anti-Keratin 8 (TROMA-1, DSHB), rabbit-anti-Keratin 14 (Abcam,

(3) Dysfunctional Extracellular Matrix Remodeling Supports Perianal Fistulizing Crohn′s Disease by a Mechanoregulated Activation of the Epithelial-to-Mesenchymal TransitionCellular and Molecular Gastroenterology and HepatologyJanuary 1, 2023Giulia Rizzo, Federica Rubbino, Sudharshan Elangovan, Giusy Sammarco, Sara Lovisa, Restelli Restelli, Samuel Pineda Pineda Chavez, Luca Massimino, Luigi Lamparelli, Marianna Paulis, et al.anti-MUC2 antibody (EPR23479-47) (1:400, ab272692; Abcam), recombinant anti-EpCAM antibody (E144) (1:200, ab32392; Abcam), anti-cytokeratin 19 (1:500 ab7754; Abcam), anti-villin (1.400, ab97512; Abcam), and anti-Zo1

(4) SOX17-positive rete testis epithelium is required for Sertoli valve formation and normal spermiogenesis in the male mouseNature communicationsDecember 21, 2022Uchida A, Imaimatsu K, Suzuki H, Han X, Ushioda H, Uemura M, Imura-Kishi K, Hiramatsu R, Takase HM, Hirate Y, et al.1:400), goad polyclonal anti-c-KIT (R&D systems, AF1356, 1:200), rabbit monoclonal anti-EPCAM (Abcam, ab32392, 1:100), goat polyclonal anti-GATA4 (Santa Cruz, sc1237, 1:200), mouse monoclonal anti-GATA4 (Santa Cruz

(5) Bladder cancer cell lines adapt their aggressiveness profile to oxygen tensionOncology LettersMay 20, 2022Stéphane Chabaud, Ève Pellerin, Christophe Caneparo, Cassandra Ringuette‑goulet, Frédéric Pouliot, Stéphane BolducMerck KGaA,"1/20,000","1/20,000",A5441 E-Cadherin,Mouse,Abcam,"1/1,000","1/1,000",Ab1416 EpCAM,Rabbit,Abcam,1/500,"1/1,000",Ab32392 N-Cadherin,Mouse,MilliporeSigma,"1/1,000","1/1,000",05-915 TSP-1,Rabbit,"Cell Signaling Technology,

(6) Human biliary epithelial cells from discarded donor livers rescue bile duct structure and function in a mouse model of biliary diseaseCell Stem CellMarch 3, 2022John M. Hallett et al.RRID:AB_303048 CD45,R&D,Cat#MAB114; RRID:AB_357485 CK19,Novocastra,Cat#NCL-CK19: RRID:AB_563799 EpCAM,Abcam,Cat#Ab32392; RRID:AB_732181 EpCAM (mouse specific),Abcam,Cat#Ab221552 CD133,Abcam,Cat#Ab19898; RRID:AB_470302 CD24,Biolegend,Cat#101803;

(7) Generation and differentiation of chemically derived hepatic progenitors from mouse primary hepatocytesSTAR ProtocolsDecember 17, 2021Yohan Kim, Jaemin Jeong, Dongho Choi(1:100 dilution),Abcam,"Cat#ab76055, RRID:AB_1310159" Rabbit anti-Epcam (1:100 dilution),Abcam,"Cat#ab32392, RRID:AB_732181" Hoechst 33342 (1:10000 dilution),Invitrogen,Cat#H3570 Mouse anti-Cytokeratin 19 (1:100

(8) Visualizing in deceased COVID-19 patients how SARS-CoV-2 attacks the respiratory and olfactory mucosae but spares the olfactory bulbCellNovember 24, 2021Khan M, Yoo SJ, Clijsters M, Backaert W, Vanstapel A, Speleman K, Lietaer C, Choi S, Hether TD, Marcelis L, et al.Triton/PBS and incubated at 4°C overnight: human ACE-2 (R&D Systems, Cat#AF933) at 1:100, EpCAM (Abcam, Cat#ab32392) at 1:100, ERMN (Thermo Fisher Scientific, Cat#PA5-58327) at 1:100, Cytokeratin 5/6 (Novus Biologicals,

(9) In Silico Identification of Potential Phosphorylation in the Cytoplasmic Domain of Epithelial Cell Adhesion MoleculeACS OmegaDecember 8, 2020Arijit Mal, Pranay Dey, Robert Michael Hayes, Justin V. McCarthy, Arjun Ray, Abhijit Dewere purchased from Calbiochem. Antibodies used in this study, -anti-EpCAM (EpICD) antibody [E144] (ab32392) and enhanced chemiluminescence (ECL) secondary antibodies, goat anti-rabbit HRP (IgG H&L) and rabbit

(10) The Human Lung Glycome Reveals Novel Glycan Ligands for Influenza A VirusScientific ReportsDecember 1, 2020Nan Jia, Lauren Byrd-Leotis, Yasuyuki Matsumoto, Chao Gao, Alexander N. Wein, Jenna L. Lobby, Jacob E. Kohlmeier, David A. Steinhauer, Richard D. Cummingsan hour at room temperature. The sections were incubated with rabbit anti-EpCAM (1:300, Abcam, Cat#: ab32392) plus (1) FITC conjugated mouse anti-LeA (1:100, Santa Cruz, cat#: sc-51512) and (2) FITC conjugated

(11) Markers and antibodies for characterization of goat mammary tissue and the derived primary epithelial cell culturesRevista Brasileira de ZootecniaJune 10, 2020Sonja Prpar Mihevc, Jernej Ogorevc, Peter DovčSanta Cruz Biotechnology Application Suggested dilution ICF, IHF 1:500 ICF, IHF ACTA2; α-SMA, A5228, Sigma CSN2; #250558, Abbiotec ICF EPCAM; E144, ab32392, Abcam ICF, IHF ESR1 (ERα); H-184: sc-7207, Santa Cruz Biotechnology ITGA6 (CD49f); H-87 : sc-10730,

(12) A Novel Function for KLF4 in Modulating the De-differentiation of EpCAM(-)/CD133(-) nonStem Cells into EpCAM(+)/CD133(+) Liver Cancer Stem Cells in HCC Cell Line HuH7CellsMay 12, 2020Karagonlar ZF, Akbari S, Karabicici M, Sahin E, Avci ST, Ersoy N, Ates KE, Balli T, Karacicek B, Kaplan KN, et al.activity. After the incubation in the blocking serum for 2 h, the samples were stained with EpCAM (#ab32392, Abcam) and KLF4 (#ab215036, Abcam) antibodies overnight at 4 ◦ C. The next day, after washing with

(13) High-grade mesenchymal pancreatic ductal adenocarcinoma drives stromal deactivation through CSF-1EMBO reportsMay 6, 2020Steins A, van Mackelenbergh MG, van der Zalm AP, Klaassen R, Serrels B, Goris SG, Kocher HM, Waasdorp C, de Jong JH, Tekin C, et al.1:1,000), CK19 (MU246‐UC, BioGenex, Fremont, CA, 1:500), E‐cadherin (EP700Y, Abcam, 1:100), EpCAM (ab32392, Abcam, 1:100), or CSF‐1 (EP1179Y, Ab52864, Abcam, 1:100) was in normal antibody diluent (KliniPath,

(14) Congenital Tufting Enteropathy-Associated Mutant of Epithelial Cell Adhesion Molecule Activates the Unfolded Protein Response in a Murine Model of the DiseaseCellsApril 11, 2020Das B, Okamoto K, Rabalais J, Marchelletta RR, Barrett KE, Das S, Niwa M, Sivagnanam M.the ratio was shown. Primary antibodies used for these experiments were: rabbit anti-EpCAM (E144) (ab32392, Abcam, Cambridge, UK), mouse anti GRP78/BiP (A10) (sc376768 Santa Cruz Biotechnology, Dallas, TX, USA),

(15) Feasibility of primary human cell cultures as a model for adamantinomatous craniopharyngioma research: Evidence from RNA‑Seq analysisOncology LettersJanuary 16, 2020Pei‑Dong Zhang, Chao‑Hu Wang, Jun Fan, Jun‑Xiang Peng, Jun Pan, Song‑Tao Qi, Yi Liu1:100; cat. no., ab7753; Abcam) and epithelial cell adhesion molecule (EpCAM; dilution 1:100, cat. no., ab32392; Abcam), followed by incubation at 25°C for 1 h with Alexa Fluor 488 anti-mouse or 594 anti-rabbit secondary

(16) A meta-analysis and The Cancer Genome Atlas data of prostate cancer risk and prognosis using epithelial cell adhesion molecule (EpCAM) expressionBMC UrologyDecember 1, 2019Yu Hu, Qiong Wu, Jialin Gao, Yongrui Zhang, Yuantao Wang(100%) Benign ≥ 25% 102 (52%) Benign ≥ 40% Normal 23 (91.3%) Abcam, cat. #11294, ≥ 20% dilution 1:200 T2-T3 ab32392, ABCAM, Cambridge, MA, dilution 1:600 NA N (%) 414 (87.2%) > 70% T1-T4 ESA, clone VU-1D9, Novocastra, Newcastle

(17) Establishment and Characterization of 5-Fluorouracil-Resistant Human Colorectal Cancer Stem-Like Cells: Tumor Dynamics under Selection PressureInternational Journal of Molecular SciencesApril 12, 2019Maria Giovanna Francipane, Denis Bulanin, Eric Lagasseab53773), Ki-67 (Abcam, cat. no. ab15580), TRA-2-49 (Abcam, cat. no. ab17973), EpCAM (Abcam, cat. no. ab32392), Muc2 (Santa Cruz, cat. no. sc-7314; Santa Cruz, CA, USA), Villin (AbD Serotec, cat. no. MAB1671; Raleigh,

(18) Comparing small urinary extracellular vesicle purification methods with a view to RNA sequencing-Enabling robust and non-invasive biomarker researchBiomolecular detection and quantificationMarch 1, 2019Mussack V, Wittmann G, Pfaffl MW.B-2, sc-271022, Santa Cruz, 1:100), rabbit anti-Alix (ab186728, Abcam, 1:1,000), rabbit anti-EPCAM (ab32392, Abcam, 1:1,000), rabbit anti-Syntenin (Clone EPR8102, ab133267, Abcam, 1:5,000), mouse anti-TSG101

(19) Lineage tracing of Notch1-expressing cells in intestinal tumours reveals a distinct population of cancer stem cellsScientific reportsJanuary 29, 2019Mourao L, Jacquemin G, Huyghe M, Nawrocki WJ, Menssouri N, Servant N, Fre S.anti-PCNA (1:1000, Abcam ab29), rabbit anti-Ki67 (1:200, ab15580), rabbit anti-EpCAM (1:400, Abcam, ab32392). Secondary antibodies were incubated in PBS for 1–2 hours at room temperature. The following secondary

(20) Statin-induced metabolic reprogramming in head and neck cancer: a biomarker for targeting monocarboxylate transportersScientific ReportsDecember 1, 2018Manal Mehibel et al.against MCT4 (sc50329, Santa Cruz biotechnology), MCT1 (sc50324, Santa Cruz biotechnology), EpCAM (ab32392, Abcam), cyclophilin A (ab41684, Abcam) and β-Actin (A1978, Sigma) overnight at 4 °C. Proteins were

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(1)

5/25/2016Detection of EpCAM in Cancer Cells via Western Blot Rocky SateOur longstanding goal is to understand the role of biomarkers in different cell lines. Here we test the detection of EpCAM, an epithelial specific marker in different colon cancer cell lines using ... Read Review
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8/7/2015Detection of EpCAM in Cancer Cells Rocky SateOur longstanding goal is to understand the role of biomarkers in different cell lines. Here we test the detection of EpCAM, an epithelial specific marker in colon cancer cell lines using ... Read Review

Anti-EpCAM antibody [E144] from Abcam

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Anti-EpCAM antibody [E144] from Abcam

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