APC anti-mouse CD3 100235 from BioLegend

Product Specs
ItemAPC anti-mouse CD3
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number100235
Quantity25 µg
ApplicationsFlow Cytometry (FC)
Conjugate/TagAPC
ReactivityMouse
IsotypeIgG2b, Kappa
Clone17A2
HostRat
NCBI Full Gene NameCD3 antigen, gamma polypeptide
NCBI Gene AliasesCtg-3, Ctg3, T3g
Concentration0.20 mg/mL
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
ImmunogenγδTCR-positive T-T hybridoma D1
Antibody TypePrimary
ClonalityMonoclonal
TargetCD3

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BioLegend

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San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
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Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) A HER2-targeting antibody-MMAE conjugate RC48 sensitizes immunotherapy in HER2-positive colon cancer by triggering the cGAS-STING pathwayCell death & diseaseAugust 24, 2023Wu X, Xu L, Li X, Zhou Y, Han X, Zhang W, Wang W, Guo W, Liu W, Xu Q, et al.Zombie NIRTM (423105, Biolegend) for dead cell preclusion, CD45-BV510 (103138, Biolegend), CD3-APC (100236, Biolegend), CD4-FITC (100406, Biolegend), CD8-BV421 (100738, Biolegend), IFN-γRα-PE (308606, Biolegend). IHC

(2) Assessing the Effects of Nicotinamide Mononucleotide Supplementation on Pulmonary Inflammation in Male Mice Subchronically Exposed to Ambient Particulate MatterEnvironmental Health PerspectivesJuly 17, 2023Rui Zhang, Shen Chen, Ziwei Wang, Lizhu Ye, Yue Jiang, Miao Li, Xinhang Jiang, Hui Peng, Zhanyu Guo, Liping Chen, et al.Antibodies used for T cells sorting was used as follows: FVD eFluor 780 (1:1,000), anti-CD3 (1:100; 100236; clone 17A2; APC; Biolegend), and anti-CD25 (1:200; 101904; clone 3C7; PE; Biolegend). Sorted T cells

(3) RAB27B controls palmitoylation-dependent NRAS trafficking and signaling in myeloid leukemiaThe Journal of clinical investigationJune 15, 2023Ren JG, Xing B, Lv K, O'Keefe RA, Wu M, Wang R, Bauer KM, Ghazaryan A, Burslem GM, Zhang J, et al.(no. 110730, 1:200), APC-Cy7-CD45.2 (no. 109824, 1:200), PE-B220 (no. 103208, 1:200), APC-CD3 (no. 100236, 1:200), PE-Gr1 (no. 108408, 1:200), and APC-Mac1 (no. 101212, 1:200) antibodies were from BioLegend. Quantitative

(4) Lymphocyte-Related Immunomodulatory Therapy with Siponimod (BAF-312) Improves Outcomes in Mice with Acute Intracerebral HemorrhageAging and diseaseJune 1, 2023Zhang Z, Li Y, Shi J, Zhu L, Dai Y, Fu P, Liu S, Hong M, Zhang J, Wang J, et al.suspension was then stained with PerCP/Cyanine5.5 anti-mouse CD45 (103132, Biolegend), APC anti-mouse CD3 (100236, Biolegend), Brilliant Violet 605™ anti-mouse CD4 (100451, Biolegend), Pacific Blue™ anti-mouse CD8α

(5) PD-L1 methylation restricts PD-L1/PD-1 interactions to control cancer immune surveillanceScience advancesMay 26, 2023Huang C, Ren S, Chen Y, Liu A, Wu Q, Jiang T, Lv P, Song D, Hu F, Lan J, et al.BioLegend), Zombie NIR Fixable Viability Kit (423106; BioLegend), APC-conjugated anti-mouse CD3 antibody (100236; BioLegend), PerCP/Cyanine5.5-conjugated anti-mouse CD45 antibody(147706; BioLegend), PE/Cy7-conjugated

(6) A novel aptamer-based small RNA delivery platform and its application to cancer therapyGenes & DiseasesMay 1, 2023Toshihiko Tanno, Peng Zhang, Christopher Bailey, Yin Wang, Wannaporn Ittiprasert, Martin Devenport, Pan Zheng, Yang LiuAPC (Cat# 553356) (BD Biosciences, San Jose, CA), and anti-mouse CD3 antibody (clone 17A2) APC (Cat#100235) (Biolegend, San Diego, CA) were used for flow cytometry. For binding analyses of cKit aptamer-miR-26a

(7) Nanoparticle-mediated TRPV1 channel blockade amplifies cancer thermo-immunotherapy via heat shock factor 1 modulationNature communicationsApril 29, 2023Li T, Jiang S, Zhang Y, Luo J, Li M, Ke H, Deng Y, Yang T, Sun X, Chen H.catalog number 141706, clone: C068C2, dilution: 1:200), APC anti-mouse CD3 (BioLegend, catalog number 100236, clone: 17A2, dilution: 1:200), PE anti-mouse CD8a (BioLegend, catalog number 100708, clone: 53-6.7,

(8) Temperature sensitive liposome based cancer nanomedicine enables tumour lymph node immune microenvironment remodellingNature CommunicationsApril 19, 2023Shunli Fu, Lili Chang, Shujun Liu, Tong Gao, Xiao Sang, Zipeng Zhang, Weiwei Mu, Xiaoqing Liu, Shuang Liang, Han Yang, et al.were of analytical reagent grade. Antibodies APC anti-mouse CD3 Antibody (BioLegend; Catalog number: 100236; Clone name: 17A2; 1:100 dilution), FITC anti-mouse CD4 Antibody (BioLegend; Catalog number: 100405;

(9) EHBP1L1 Drives Immune Evasion in Renal Cell Carcinoma through Binding and Stabilizing JAK1Advanced science (Weinheim, Baden-Wurttemberg, Germany)April 1, 2023Pan Y, Shu G, Fu L, Huang K, Zhou X, Gui C, Liu H, Jin X, Chen M, Li P, et al.PE anti‐human IFN‐γ (502508, Biolegend), APC anti‐human CD3 (317317, Biolegend), APC anti‐mouse CD3 (100235, Biolegend), FITC anti‐human PD‐L1 (393605, Biolegend) and FITC anti‐human HLA‐A2 (343303, Biolegend).

(10) Active recruitment of anti–PD-1–conjugated platelets through tumor-selective thrombosis for enhanced anticancer immunotherapyScience AdvancesMarch 31, 2023Yixin Wang, Wen Li, Zhaoting Li, Fanyi Mo, Yu Chen, Mari Iida, Deric L Wheeler, Quanyin HuRMP1-14, category no.: 114114), allophycocyanin (APC)–anti-mouse CD3 (clone no.: 17A2, category no.: 100236), phycoerythrin (PE)–anti-mouse CD8a (clone no.: 53-6.7, category no.: 100708), and FITC–anti-mouse

(11) Supplementation with α-ketoglutarate improved the efficacy of anti-PD1 melanoma treatment through epigenetic modulation of PD-L1Cell death & diseaseFebruary 28, 2023Liu N, Zhang J, Yan M, Chen L, Wu J, Tao Q, Yan B, Chen X, Peng C.stained using APCCY7-CD45 antibody (BioLegend, CAT#:103116, USA), APC-CD3 antibody (BioLegend, CAT#:100236, USA), PE-Cy5.5-CD4 antibody (BioLegend, CAT#:100434 USA), PE-Cy7-CD8 antibody (BioLegend, CAT#:100722,

(12) Targeting Menin disrupts the KMT2A/B and polycomb balance to paradoxically activate bivalent genesNature cell biologyFebruary 1, 2023Sparbier CE, Gillespie A, Gomez J, Kumari N, Motazedian A, Chan KL, Bell CC, Gilan O, Chan YC, Popp S, et al.(BioLegend, Cat# 103108; clone 30-F11; RRID: AB_312973), APC monoclonal rat anti-mouse CD3 (BioLegend, Cat# 100236; clone 17A2; RRID: AB_2561456), PE monoclonal rat anti-mouse CD8a (BioLegend, Cat# 100708; clone 53-6.7;

(13) Platelet-Derived Mitochondria Attenuate 5-FU-Induced Injury to Bone-Associated Mesenchymal Stem CellsStem cells internationalJanuary 1, 2023Chen Y, Liu Z, An N, Zhang J, Meng W, Wang W, Wu X, Hu X, Chen Y, Yin W.analysis were as follows: CD11b-APC (#101212, Biolegend), Gr-1-APC (#108412, Biolegend), CD3-APC (#100236, Biolegend), CD19-APC (#17-0193-80, eBioscience), Ter119-APC (#17-5921, eBioscience), B220-APC (#103212,

(14) IRE1α overexpression in malignant cells limits tumor progression by inducing an anti-cancer immune responseOncoImmunologyDecember 31, 2022Adriana Martinez-Turtos et al.anti-CD47,"REAfinity, Miltenyi Biotec","130–103-105, RRID:AB_2659751" APC anti-CD3,BioLegend,"Cat# 100236, RRID:AB_2561456" PE-Vio770 anti-CD8a,"REAfinity, Miltenyi Biotec","130–119-123, RRID:AB_2733250" V450

(15) FOXO1 Alleviates Liver Ischemia-reperfusion Injury by Regulating the Th17/Treg Ratio through the AKT/Stat3/FOXO1 PathwayJournal of Clinical and Translational HepatologyDecember 28, 2022Hao-zhen Ren, Sen-zhe Xia, Xue-qian Qin, An-yin Hu, Jing-lin Wanganti-mouse CD4 (100405; Biolegend, San Diego, CA, USA), allophycocyanin-conjugated anti-mouse CD3 (100235; Biolegend, San Diego, CA, USA), allophycocyanin-conjugated anti-mouse CD25 (101909; Biolegend, San

(16) HDAC5 Loss Enhances Phospholipid-Derived Arachidonic Acid Generation and Confers Sensitivity to cPLA2 Inhibition in Pancreatic CancerCancer ResearchDecember 16, 2022Penglin Pan, Gengdu Qin, Bo Wang, Haixin Yu, Jie Chen, Jiaying Liu, Kaijian Bing, Jian Shen, Dianyun Ren, Yuhan Zhao, et al.and stained for flow cytometry with the following antibodies: APC-conjugated CD3 antibody (BioLegend, 100236); FITC-conjugated CD4 antibody (BioLegend, 100510); PerCP-conjugated CD45 antibody (BioLegend, 103130);

(17) PRC2-Mediated Epigenetic Suppression of Type I IFN-STAT2 Signaling Impairs Antitumor Immunity in Luminal Breast CancerCancer researchDecember 16, 2022Hong J, Lee JH, Zhang Z, Wu Y, Yang M, Liao Y, de la Rosa R, Scheirer J, Pechacek D, Zhang N, et al.(BioLegend, #103116), anti-CD4 (BioLegend, #100510), anti-CD8 (BioLegend, #100708), and anti-CD3 (BioLegend, #100236)] for 20–30 minutes on ice. Antibodies used in CyTOF were listed in Supplemental Table 2. For intracellular

(18) Sialyltransferase Inhibitor Ac(5)3F(ax)Neu5Ac Reverts the Malignant Phenotype of Pancreatic Cancer Cells, and Reduces Tumor Volume and Favors T-Cell Infiltrates in MiceCancersDecember 12, 2022Miró L, López J, Guerrero PE, Martínez-Bosch N, Manero-Rupérez N, Moreno M, Ortiz MR, Llop E, Navarro P, Peracaula R.κ",1/1000,Spectral FC,"Cat# 100751, BioLegend" APC anti-mouse CD3 Antibody),"Rat IgG2b, κ",1/500,Spectral FC,"Cat# 100235, BioLegend" PerCP-Cy™5.5 Rat Anti-Mouse CD45,Rat LOU,1/200,Spectral FC,"Cat# 567310, BD Biosciences" APC-Cyanine7

(19) Depletion of tumor associated macrophages enhances local and systemic platelet-mediated anti-PD-1 delivery for post-surgery tumor recurrence treatmentNature CommunicationsDecember 1, 2022Zhaoting Li, Yingyue Ding, Jun Liu, Jianxin Wang, Fanyi Mo, Yixin Wang, Ting-Jing Chen-Mayfield, Paul M. Sondel, Seungpyo Hong, Quanyin HuPerCP/Cy5.5-anti-human/mouse CD11b (BioLegend, M1/70, 101227), APC-anti-mouse CD3 (BioLegend, 17A2, 100236), FITC-anti-mouse CD4 (BioLegend, GK1.5, 100406), PE-anti-mouse CD8a (BioLegend, 53-6.7, 100708), FITC-anti-mouse

(20) T cells specific for α-myosin drive immunotherapy-related myocarditisNatureNovember 1, 2022Axelrod ML, Meijers WC, Screever EM, Qin J, Carroll MG, Sun X, Tannous E, Zhang Y, Sugiura A, Taylor BC, et al.cells were incubated for 20 minutes at 4°C. Surface antibodies used were anti-CD3-APC (BioLegend, cat# 100235, clone 17A2, dilution 1:400) and anti-CD8a-FITC (Thermo Fisher, cat # MA5-16759, clone KT15, dilution

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12/17/2015Detection of CD3 Positive T Cells in Mouse Mammary Tumors Dinesh AhirwarThe mouse orthotopic syngeneic mammary tumors established by MVT1 cells were analyzed for the presence of T cells using APC anti-mouse CD3 Antibody antibody and flow cytometry. the tumors were ... Read Review

APC anti-mouse CD3 from BioLegend

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APC anti-mouse CD3 from BioLegend

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