Brilliant Violet 510(TM) anti-mouse CD90.2 (Thy1.2) 140319 from BioLegend

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Nature communications
July 28 2020

Fig 1: The intestinal IEL compartment is shaped by expression of distinct Btnl molecules.a Targeting strategy to generate conditional and constitutive Btnl1 and Btnl6 knockout mice. Depending on the Cre-transgenic strain used (i/ii) resulting animals are either ubiquitously deleted for the respective Btnl gene (?/?), or harbour a tissue-specific deletion (?gut). Black: translated exons, grey: untranslated regions, blue triangles: loxP sites. b Quantification of V?7+ IEL (black gate in c) in Btnl1 and Btnl6-deficient strains. Data are mean ± SEM of =2 independent experiments. nctrl: 32, nBtnl1-KOMP: 4, nBtnl1?gut: 8, nBtnl6?gut: 8, nBtnl6??: 9. Statistical analysis: Kruskal–Wallace and Dunn’s multiple comparison analysis. c FACS profiles of IEL preparations from animals of indicated genotypes, gated on TCR?d+ cells. The label Btnl1Komp indicates germline Btnl1KO animals generated by the IMPC that have been described16. Black gate: all V?7+ cells, green gate: V?7+Vd4+ cells. d Quantification of IEL: V?7+ IEL numbers (top), %V?7+ IEL (middle) and %CD122+ cells (bottom) in animals of indicated genotypes. Data are mean ± SD of a representative experiment. Top panel: nctrl: 12, nBtnl1: 2, nBtnl6?gut: 2, nBtnl6?/?: 3. Statistical analysis: Kruskal–Wallace & Dunn’s multiple comparison. Middle and bottom panel: nctrl: 12, nBtnl1?gut: 3, nBtnl6?gut: 2, nBtnl6?/?: 3. Statistical analysis: Kruskal–Wallace & Dunn’s multiple comparison. e Histogram for surface expression of CD122 in V?7+ IEL from animals of the indicated genotypes. f Scheme depicting the strategy to generate Btnl146Indel mice. Short guide RNAs flanking the 5' region of Btnl1 and 5' region of Btnl6 were injected with HDR templates. Due to the nature of CRISPR/Cas9 the intervening region was excised and a Btnl146INDEL mouse lacking the Btnl1-4-6 locus was created. Blue triangle: loxP site that was inserted due to the nature of the HDR template (see Methods). g FACS analysis of TCRß-CD3+ IEL in Btnl1/4/6-KO and Btnl146INDEL mice. Colours correspond to coloured bar graphs in (h) and (i). h Quantification of V?7+ IEL depicted in (g). Data are mean ± SEM of three independent experiments. nCtrl: 12, nBtnll1?/?: 7, nBtnl4KOMP: 7, nBtnl6?gut: 11, nBtnl146Indel: 10. Statistical analysis: Kruskal–Wallace & Dunn’s multiple comparison. i Surface expression of CD122 (left) and Thy1.2 (right) in V?7+ IEL from animals of indicated genotypes.
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Cell
September 22 2016

Fig 2: Btnl1 Drives Selective Expansion and Maturation of Gut IEL(A) 3 hr EdU incorporation in vivo in ?d IEL subsets and thymocytes from week 4 WT versus Btnl1-/- mice (n = 9). (B) Surface phenotypes of V?7+ and V?7GL2+ IELs from week 3–5 WT and Btnl1-/- mice (n = 8).(C) Top: Thy1 and CD122 expression by V?7+ IEL from day 35 Btnl1-/- mice (n = 8). Bottom: surface phenotypes of CD122HIThy1- and CD122LOThy1+ V?7+ IELs from week 4–6 Btnl1-/- mice (n = 4). W, week.(D and E) IEL reconstitution and CD122 profiles in (D) irradiated TCRd-/- mice 9–10 weeks post-BM transfers from indicated donors (n = 7) and (E) irradiated CD45.2+ WT or Btnl1-/- mice 4–5 weeks post-BM transfers from CD45.1+ C57Bl/6 mice (n = 7).Data are representative of two (D and E) or three or more (C, top) independent experiments. Panels (A) and (C) (bottom) present data pooled from three or more experiments.All error bars represent mean ± SD. See also Figure S4.
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Nature Communications
October 20 2022

Fig 3: Acute Tet1/2/3 depletion induces altered expression of many genes, including downregulation of mRNAs encoding the SCMC subunits KHDC2 and KHDC3.A Volcano plot of differentially expressed genes (DEGs) in acutely-deleted Tet iTKO relative to Ctrl mESC. The data are from Smart-Seq2 libraries prepared from sorted H2B-EGFP + /CD90.2-/SSEA1 + /CD326 + Ctrl or Tet iTKO mESC at day 6.5 post-4-OHT exposure. Each point represents an individual transcript. Red dots represent transcripts exhibiting log2 fold change (log2FC) of +/-1 and a pAdj value <0.05 (calculated by adjusting the p-value with the false discovery rate [FDR]). Arrows pinpoint key DEGs. Khdc2 (Ooep), Khdc3 (Filia) and Nlrp4f mRNAs, which are downregulated in Tet iTKO compared to Ctrl mESC and encode core (KHDC2) and more peripheral (KHDC3, NLRP4F) components of the subcortical maternal complex (SCMC), are highlighted. Data are from three independent experiments. B MA plot of the same RNA-Seq data, again pinpointing key DEGs and highlighting Khdc2, Khdc3 and Nlrp4f. C List of downregulated genes whose products are components of, or function in, mitosis/ meiosis, chromosome segregation, DNA replication/ repair, or other aspects of genome integrity. Khdc2 and Khdc3 are highlighted. D qRT-PCR confirming downregulation of Khdc2 and Khdc3 mRNA in Ctrl and Tet iTKO mESC 6.5 days after 4-OHT exposure (n = 3). Bar graphs represent the mean +/- standard error. The p-value was calculated using two-tailed student’s t-test (**p < 0.01; ***p < 0.001). E Time course of downregulation of Khdc2 and Khdc3 mRNA in Ctrl and Tet iTKO mESC (data are from a single experiment).
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Nature Communications
October 20 2022

Fig 4: Generation of a tamoxifen-inducible Tet1/2/3 triple-deletion system in mESC.A Mouse strains expressing the CreERT2 recombinase for inducible deletion were established in house and used to derive mESC populations that were grown under 2i conditions. Genotypes were Control (Ctrl): CreERT2; Rosa26-LSL-H2b-Egfp; Tet triple floxed (Tet Tfl): Tet1/2/3fl/fl; CreERT2; Rosa26-LSL-H2b-Egfp. Acute deletion of all three Tet genes to yield Tet1/2/3 inducible knockout (Tet iTKO) mESC, with concomitant expression of the H2B-eGFP reporter, was accomplished by culturing the cells for 2.5 days in 4-OHT, followed by 4-OHT removal and culture for an additional 4 days before analysis. B Genome browser views of Tet1 and Tet2 transcripts generated by RNA-Seq analysis of Ctrl (black tracks) and Tet iTKO (red tracks) mESC. Note that the deleted exons encode the TET catalytic domains (purple bar, bottom). C Loss of TET1 and TET2 protein expression confirmed by western blot on day 6.5 after exposure of Ctrl and Tet iTKO mESC to 4-OHT. ACTIN was used as a loading control. D Flow cytometric evaluation of 5hmC levels in Ctrl and Tet iTKO mESC. E Quantification of the loss of 5hmC in Tet iTKO mESC in duplicate experiments, relative to Ctrl and Dnmt1, Dnmt3a, Dnmt3b triple knockout (Dnmt TKO) mESC (n = 2). Signals detected from mESC stained using only the secondary antibody were also included as negative controls (n = 3). Bar graphs represent the mean +/- standard error. Tet iTKO mESC were gated on H2B-EGFP + /CD90.2-/SSEA1 + /CD326 + cells (see Methods and Supplementary Fig. 2).

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Brilliant Violet 510(TM) anti-mouse CD90.2 (Thy1.2)

BioLegend's Brilliant Violet 510(TM) anti-mouse CD90.2 (Thy1.2) is a Rat monoclonal antibody. The Brilliant Violet 510(TM) anti-mouse CD90.2 (Thy1.2) Antibody was generated using Thy1 as the antigen. It reacts with Mouse. This antibody has been shown to work in applications such as: Flow Cytometry.

Description

Brilliant Violet 510(TM) anti-mouse CD90.2 (Thy1.2) [53-2.1]

Product Specs
ItemBrilliant Violet 510(TM) anti-mouse CD90.2 (Thy1.2)
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number140319
Quantity125 µL
ApplicationsFlow Cytometry (FC)
Conjugate/TagBrilliant Violet 510™
ReactivityMouse
IsotypeIgG2a, Kappa
Clone53-2.1
HostRat
NCBI Full Gene Namethymus cell antigen 1, theta
NCBI Gene AliasesCD90, T25, Thy-1, Thy-1.2, Thy1.1, Thy1.2
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
ImmunogenMouse thymus or spleen
Antibody TypePrimary
ClonalityMonoclonal
TargetCD90.2

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(1) Human IRF1 governs macrophagic IFN-γ immunity to mycobacteriaCellFebruary 2, 2023Rosain J, Neehus AL, Manry J, Yang R, Le Pen J, Daher W, Liu Z, Chan YH, Tahuil N, Türel Ö, et al.105012, RRID:AB_493342" Brilliant Violet 510 Murine CD90.2 (Thy-1.2) (clone 53-2.1),BioLegend,"Cat# 140319, RRID:AB_2561395" APC Murine CD117 (c-Kit) (clone 2B8),Thermo Fisher Scientific,"Cat# 17-1171-82, RRID:AB_469430" Brilliant

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(3) Acute deletion of TET enzymes results in aneuploidy in mouse embryonic stem cells through decreased expression of Khdc3Nature CommunicationsOctober 20, 2022Romain O. Georges, Hugo Sepulveda, J. Carlos Angel, Eric Johnson, Susan Palomino, Roberta B. Nowak, Arshad Desai, Isaac F. LÃ³pez-Moyado, Anjana Raoin FACS buffer (PBS 1×, 0.5% BSA, 0.01% NaN3): SSEA-1 (BD Biosciences, 562705), CD90.2 (Biolegend, 140319) and CD326 (Biolegend, 118227). For detection of intracellular markers, mESC stained with surface markers

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(5) Optimized protocol for immunophenotyping of melanoma and tumor-bearing skin from mouseSTAR protocolsSeptember 17, 2021Krisna SS, Goncalves C, Gagnon N, Huang F, Plourde D, Miller WH Jr, Fritz JH, Del Rincon SV.C398.4A),eBioscience,Cat #17-9949-82 Mouse monoclonal anti-Thy 1(CD90.2) BV510 (clone 53-2.1),BioLegend,Cat #140319 Mouse monoclonal anti-CD25 PE-Cy7 (clone PC61),BD Biosciences,Cat #561780 Mouse monoclonal anti-FcεR1

(6) Butyrophilin-like proteins display combinatorial diversity in selecting and maintaining signature intraepithelial γδ T cell compartmentsNature communicationsJuly 28, 2020Jandke A, Melandri D, Monin L, Ushakov DS, Laing AG, Vantourout P, East P, Nitta T, Narita T, Takayanagi H, et al.BV421,H57-597,BioLegend,109229,1:300 CD122 PE,TM-β1,BioLegend,123209,1:200 Thy1.2 BV 510,53-2.1,BioLegend,140319,1:800 Lag3PerCPefluor 710,C9B7W,eBioscience,46-2231-80,1:300 CD24-BV650,M1/69,BD,563545,1:400 CD8α

(7) The introduction of mesenchymal stromal cells induces different immunological responses in the lungs of healthy and M. tuberculosis infected micePloS oneJanuary 1, 2017Nenasheva T, Nikolaev A, Diykanov D, Sukhanova A, Tcyganov E, Panteleev A, Bocharova I, Serdyuk Y, Nezlin L, Radaeva T, et al.

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(12) Flt3 Ligand Regulates the Development of Innate Lymphoid Cells in Fetal and Adult MiceJournal of immunology (Baltimore, Md. : 1950)March 15, 2016Baerenwaldt A, von Burg N, Kreuzaler M, Sitte S, Horvath E, Peter A, Voehringer D, Rolink AG, Finke D.

(13) Intravaginal Chlamydia trachomatis Challenge Infection Elicits TH1 and TH17 Immune Responses in Mice That Promote Pathogen Clearance and Genital Tract DamagePloS oneJanuary 1, 2016Vicetti Miguel RD, Quispe Calla NE, Pavelko SD, Cherpes TL.

(14) Polyamine induced aggregation of DNANucleic acids researchMarch 1, 1977A Osland, K Kleppe

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10/11/2022Great THy1.2 Antibody with Strong Staining Tin NgoWe are interested in studying cancer and cancer immunology in the lab. We take the spleen of mice and stain with a panel of antibodies. This is a good Thy1.2 antibody with great signal, would ... Read Review
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3/15/2022Great BioLegend Antibody For Flow Cytometry Jessica XuI use this antibody from BioLegend to stain immune cells from mouse spleen for flow cytometry. It works great and the staining is strong.
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4/28/2021Great Antibody for Thy1.2 Hong SunWe are interested in characterizing the immune populations in secondary lymphoid organs under different knockout condition.
(1)

11/26/2020Great Way to Gate Out the Thy1.1 and Thy1.2 Cells Meng SunOur lab likes to use this combination, Thy1.2 in BV510, so that we can easily distinguish Thy1.1 and Thy1.2 cells. When we transfer transgenic P14/OT1 cells into a WT host, a great way to track it is ... Read Review
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1/26/2016Brilliant Violet 510 Anti-Mouse CD90.1 (Thy1.2) Review Joanna KreitingerWe are evaluating cell population frequencies from murine thymuses. Single cell suspensions were made from whole thymuses and flow cytometry was used for surface protein evaluation.

Nature communications

Cell

Nature Communications

Nature Communications

Brilliant Violet 510(TM) anti-mouse CD90.2 (Thy1.2) from BioLegend

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Brilliant Violet 510(TM) anti-mouse CD90.2 (Thy1.2) from BioLegend

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