PerCP/Cyanine5.5 anti-mouse IL-10 505028 from BioLegend

Product Specs
ItemPerCP/Cyanine5.5 anti-mouse IL-10
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number505028
Quantity100 µg
ApplicationsIntracellular Staining for Flow Cytometry (ICFC)
Conjugate/TagPERCP_CYANINE5.5
ReactivityMouse
IsotypeIgG2b, Kappa
CloneJES5-16E3
HostRat
NCBI Full Gene Nameinterleukin 10
NCBI Gene AliasesCSIF, If2a, Il-10
Concentration0.20 mg/mL
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
ImmunogenE. coli-expressed, recombinant mouse IL-10
Antibody TypePrimary
ClonalityMonoclonal
TargetIL-10

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Centrin-deficient Leishmania mexicana confers protection against New World cutaneous leishmaniasisnpj VaccinesDecember 1, 2022Greta Volpedo et al.these antigens: FITC-IFN-γ (Biolegend Cat#505806, clone: XMG1.2), PerCP/Cy5.5-IL-10 (Biolegend Cat#505027, clone: JES5-16E3), and PE-IL-4 (Biolegend Cat#504103, clone: 11B11) (1ul per 1 × 106 cells). The following

(2) The Role of Neutrophils in Oncolytic Orf Virus-Mediated Cancer ImmunotherapyCellsSeptember 14, 2022Jessica A. Minott, Jacob P. van Vloten, Lily Chan, Yeganeh Mehrani, Byram W. Bridle, Khalil Karimiseveral panels for intracellular PE-TNF-α (eBioscience, Cat#12-7321-82), PerCP5.5-IL-10 (BioLegend, Cat#505028), APC-IL-17a (eBioscience, Cat#17-7177-81), PE-IFN-γ (BioLegend, Cat#505808), APC-IL-12p40 (BioLegend,

(3) Lack of Functional P110δ Affects Expression of Activation Marker CD80 but Does Not Influence Functions of NeutrophilsInternational journal of molecular sciencesJune 7, 2022Manda-Handzlik A, Mroczek A, Kuźmicka W, Cieloch A, Homoncik Z, Muchowicz A, Demkow U, Wachowska M.(11-7061-41; eBioscience; 1:400), anti-IL-1β PE (12-7114-80; eBioscience; 1:800), anti-IL-10 PerCP-Cy5.5 (505028; BioLegend; 1:400) and anti-IL-17A APC-Cy7 (560821; BD Pharmigen; 1:200) for 30 min at 4 °C. Cells were

(4) The subunits of IL-12, originating from two distinct cells, can functionally synergize to protect against pathogen dissemination in vivoCell reportsOctober 12, 2021Gerber AN, Abdi K, Singh NJ.11B11,Biolegend,Cat# 504104; RRID:AB_315318 PerCP/Cyanine5.5 anti-mouse IL-10 clone JES5–16E3,Biolegend,Cat# 505028; RRID:AB_2561523 APC anti-mouse IL-17A clone TC11–18H10.1,Biolegend,Cat# 506916; RRID:AB_536018 PE

(5) Bach2 attenuates IL-2R signaling to control Treg homeostasis and Tfr developmentCell reportsMay 11, 2021Zhang H, Dai D, Hu Q, Yang F, Xue Y, Li F, Shen N, Zhang M, Huang C.

(6) Sex Differences in Macrophage Responses to Obesity-Mediated Changes Determine Migratory and Inflammatory TraitsJournal of immunology (Baltimore, Md. : 1950)January 1, 2021Chen KE, Lainez NM, Coss D.IL-6 (anti-IL6 APC, 1:400, 504507, Biolegend, San Diego, CA ), IL-10 (anti-IL10 PerCP/Cy5.5, 1:400, 505027, Biolegend, San Diego, CA) and TNFα (anti-TNFα FITC, TN3–19.12, 11–7423-82 eBioscience, San Diego, CA).

(7) Acetylation-dependent regulation of PD-L1 nuclear translocation dictates the efficacy of anti-PD-1 immunotherapyNature cell biologySeptember 1, 2020Gao Y, Nihira NT, Bu X, Chu C, Zhang J, Kolodziejczyk A, Fan Y, Chan NT, Ma L, Liu J, et al.anti-IFNγ FITC (#505806, Biolegend); anti-IL-2 BV510 (#503833, Biolegend); anti-IL10 PerCP-Cy5.5 (#505028, Biolegend). All analyses were performed using FlowJo_V10.6.1 software (Tree Star). Gating strategy:

(8) Diverse homeostatic and immunomodulatory roles of immune cells in the developing mouse lung at single cell resolutioneLifeJune 2, 2020Domingo-Gonzalez R, Zanini F, Che X, Liu M, Jones RC, Swift MA, Quake SR, Cornfield DN, Alvira CM.,BD Biosciences,Cat# 562045; RRID:AB_10895799,FACS (1:100) Antibody,IL-10 (Rat monoclonal),BioLegend,Cat# 505027; RRID:AB_2561522,FACS (1:100) Antibody,IL-17 (Rat monoclonal),Miltenyi Biotec,Cat# 130-095-732; RRID:AB_10828821,FACS

(9) Peripherally derived angiotensin converting enzyme-enhanced macrophages alleviate Alzheimer-related diseaseBrain : a journal of neurologyJanuary 1, 2020Koronyo-Hamaoui M, Sheyn J, Hayden EY, Li S, Fuchs DT, Regis GC, Lopes DHJ, Black KL, Bernstein KE, Teplow DB, et al.ImmunoResearch Laboratories eBioscience/ThermoFisher eBioscience/ThermoFisher eBioscience/ThermoFisher Covance/BioLegend BioLegend eBioscience/ThermoFisher BioLegend BioLegend 25-0112-81 12-0451-81 11-4801-81 803003 505028 48-5932-80 141410 506308 Primary antibodies Arginase1 pAb Beclin1 pAb CD163 pAb CD204 mAb Scavenger

(10) Compromised Metabolic Reprogramming Is an Early Indicator of CD8⁺ T Cell Dysfunction during Chronic Mycobacterium tuberculosis InfectionCell reportsDecember 10, 2019Russell SL, Lamprecht DA, Mandizvo T, Jones TT, Naidoo V, Addicott KW, Moodley C, Ngcobo B, Crossman DK, Wells G, et al.(MP6-XT22) BV11 Anti-mouse,Biolegend,Cat# 506349 anti-IL-10 (JES5-16E3) PerCP/ Cy5.5,Biolegend,Cat# 505028 anti-IL-2 (JES6-5H4) FITC,Biolegend,Cat# 503805 anti-IL-2 (JES6-5H4) PE-Dazzle 594,Biolegend,Cat#

(11) Intermittent Fasting Confers Protection in CNS Autoimmunity by Altering the Gut MicrobiotaCell metabolismJune 5, 2018Cignarella F, Cantoni C, Ghezzi L, Salter A, Dorsett Y, Chen L, Phillips D, Weinstock GM, Fontana L, Cross AH, et al.Cat 506907 IFN-γ APC,Biolegend,Clone XMG1.2 Cat 505809 IL-10 PercP,Biolegend,Clone JES5–16E3 Cat 505027 GM-CSF PE,Biolegend,Clone MP1–22E9 Cat 505405 Zombie Acqua,Biolegend,Cat 423101 Human CD303 (BDCA-2)

(12) Age-Related Differential Stimulation of Immune Response by Babesia microti and Borrelia burgdorferi During Acute Phase of Infection Affects Disease SeverityFrontiers in immunologyJanuary 1, 2018Djokic V, Primus S, Akoolo L, Chakraborti M, Parveen N.with BV605 (BioLegend #504126), anti-mouse IL-10 antibodies conjugated with PerCP-Cy5.5 (BioLegend #505028), anti-mouse IL-21 antibodies conjugated with eFluor 660 (ThermoFisher #50-7211-82) all used at 1:50

(13) The mito-DAMP cardiolipin blocks IL-10 production causing persistent inflammation during bacterial pneumoniaNature communicationsJanuary 11, 2017Chakraborty K, Raundhal M, Chen BB, Morse C, Tyurina YY, Khare A, Oriss TB, Huff R, Lee JS, St Croix CM, et al.

(14) Mir-223 regulates the number and function of myeloid-derived suppressor cells in multiple sclerosis and experimental autoimmune encephalomyelitisActa neuropathologicaJanuary 1, 2017Cantoni C, Cignarella F, Ghezzi L, Mikesell B, Bollman B, Berrien-Elliott MM, Ireland AR, Fehniger TA, Wu GF, Piccio L.intracellular cytokines encompassed anti-mouse antibodies to IL-17A (Biolegend, 506908), IL-10 (Biolegend, 505028), GM-CSF (Biolegend, 505406), IFN-γ (Biolegend, 505810), and TNF-α (eBioscience 11-7321-81). Isolated

(15) Familial Alzheimer's Disease Mutations in Presenilin Generate Amyloidogenic Aβ Peptide SeedsNeuronApril 20, 2016Veugelen S, Saito T, Saido TC, Chávez-Gutiérrez L, De Strooper B.

(16) [Beta blockaders in different forms of angina]CardiologyJanuary 1, 1978P Groussin

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(1)

3/18/2020Not Very Bright but Fair Anti-IL-10 PerCP Cy5.5 Elodie MohrI could identify and quantify IL10-producing CD4 T cells in the spleen.

PerCP/Cyanine5.5 anti-mouse IL-10 from BioLegend

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PerCP/Cyanine5.5 anti-mouse IL-10 from BioLegend

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