Anti-AKT3 + AKT2 + AKT1 antibody [Y89] ab32505 from Abcam

Product Specs
ItemAnti-AKT3 + AKT2 + AKT1 antibody [Y89]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab32505
Antibody TypePrimary
CloneY89
ClonalityMonoclonal
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunoprecipitation, Western blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunoprecipitation, Western blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunoprecipitation, Western blot
IsotypeIgG
Quantity10 UL, 40 UL, 100 UL
HostRabbit
Conjugate/TagUnconjugated
Gene NameAKT1
ReactivityHuman
TargetAKT3+AKT2+AKT1

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Regulation of Osteoimmune Microenvironment and Osteogenesis by 3D‐Printed PLAG/black Phosphorus Scaffolds for Bone RegenerationAdvanced ScienceOctober 1, 2023Jing Long, Zhenyu Yao, Wei Zhang, Ben Liu, Kaiming Chen, Long Li, Bin Teng, Xiang‐Fu Du, Cairong Li, Xue‐Feng Yu, et al.were obtained from Abcam, including Anti‐PI 3 Kinase p85 alpha (ab182651), anti‐protein kinase B (AKT, ab32505), anti‐p‐AKT (S473) (ab66138), Runx2 (ab76956), BMP2 (ab6285), β‐actin (ab8226), goat anti‐mouse IgG H&L (HRP

(2) FGFR1 is a potential therapeutic target in neuroblastomaCancer Cell InternationalDecember 1, 2022Flora Cimmino et al.anti-pSTAT3 (D3A7, Cell Signaling), anti-STAT3 (06596, Millipore), anti-pAKT1 (ab81283; Abcam), anti-AKT1 (ab32505; Abcam), anti-pERK1/2 (ab32538; Abcam), anti-ERK1/2 (ab17942; Abcam), anti t-GFP (TA150041, Origene)

(3) Different Inhibition of Nrf2 by Two Keap1 Isoforms α and β to Shape Malignant Behaviour of Human Hepatocellular CarcinomaInternational Journal of Molecular SciencesSeptember 7, 2022Feilong Chen, Mei Xiao, Jing Feng, Reziyamu Wufur, Keli Liu, Shaofan Hu, Yiguo ZhangFG4R",Invitrogen α-Tubulin,ab52866,Abcam Histone 3,ab192985,Abcam PTEN,ab267787,Abcam PI3K,ab245781,Abcam AKT1,ab32505,Abcam mTOR,ab134903,Abcam SNAI1,A00716-2,BioSynthesis SNAI2,PB9439,BOSTER CDH2,ab76011,Abcam FN1,BA1772,BOSTER MMP9,BA0573,BOSTER Vimentin,Ab92547,Abcam Fluorescent

(4) PEBP4 Directs the Malignant Behavior of Hepatocellular Carcinoma Cells via Regulating mTORC1 and mTORC2International journal of molecular sciencesAugust 8, 2022Chen Q, Jin J, Guo W, Tang Z, Luo Y, Ying Y, Lin H, Luo Z.Bridge (Beijing, China)" PEBP4,ab139074,"Abcam (Cambridge, UK)" p-SGK1 (Ser422),ab55281,Abcam Akt,ab32505,Abcam Rictor,ab70374,Abcam SGK1,28454-1-AP,"Proteintech (Wuhan, China)" PKCα,21991-1-Ig,Proteintech RSK2,23762-1-AP,Proteintech mTOR

(5) Telomerase RNA TERC and the PI3K-AKT pathway form a positive feedback loop to regulate cell proliferation independent of telomerase activityNucleic acids researchApril 22, 2022Wu S, Ge Y, Lin K, Liu Q, Zhou H, Hu Q, Zhao Y, He W, Ju Z.for, p-S473 AKT (4060, Cell Signaling Technology), p-T308 AKT (13038, Cell Signaling Technology), AKT (ab32505, Abcam), p-S9 GSK3β (9322, Cell Signaling Technology), GSK3β (9315, Cell Signaling Technology), PDPK1

(6) Chemoprevention of 4NQO-Induced Mouse Tongue Carcinogenesis by AKT Inhibitor through the MMP-9/RhoC Signaling Pathway and AutophagyAnalytical cellular pathology (Amsterdam)January 1, 2022Yin P, Chen J, Wu Y, Gao F, Wen J, Zhang W, Su Y, Zhang X.which were then blocked with 5% skimmed milk and incubated with primary antibodies (anti-AKT, 1 : 5000, ab32505, Abcam, Cambridge, MA; anti-phospho-AKT (S473), 1 : 5000, ab81283, Abcam; anti-MMP-9, 1 : 1000, ab137867,

(7) KLF5 activates lncRNA DANCR and inhibits cancer cell autophagy accelerating gastric cancer progressionnpj Genomic MedicineDecember 1, 2021Zhiyi Cheng, Guiyuan Liu, Chuanjiang Huang, Xiaojun Zhaofor 1 h. The blots were probed with dilute primary antibody rabbit anti-KLF5 (ab137676, 1:100), AKT2 (ab32505, 1:3000), LC3B (ab51520, 1:3000, Abcam, UK), Beclin-1 (ab207612, 1:2000, Abcam, UK), and GAPDH (ab181602,

(8) Andrographolide Attenuates Established Pulmonary Hypertension via Rescue of Vascular RemodelingBiomoleculesNovember 30, 2021Nie X, Shen C, Tan J, Yang X, Wang W, Dai Y, Sun H, Wu Z, Chen J.ab7970), histone (1:1000; Abcam Cat# ab13924), p-AKT (1:1000; Abcam Cat# ab81283), AKT (1:1000; Abcam Cat# ab32505), p-ERK1/2 (1:2000, Cell Signaling Technology Cat #4370, Danvers, MA, USA), ERK1/2 (1:2000, Cat # 4695,

(9) Induction of mitochondrial-dependent apoptosis by essential oil of Toona sinensis root through Akt, mTOR and NF-κB signalling pathways in human renal cell carcinoma cellsJournal of Food and Drug AnalysisSeptember 15, 2021Yu-Chi Chen, Chiao-Ling Hsieh, Bu-Miin Huang, Yung-Chia ChenPrimary antibodies,dilution,Company "pAkt1 (Ser473) (ab81283) Akt1 (ab32505), mTOR (ab134903), cleaved PARP-1 (ab32064), NF-κB (ab32536), Hsp90 (ab203126)",1:1000,"Abcam Plc. (Cambridge,

(10) Dexmedetomidine ameliorates lipopolysaccharide-induced acute lung injury by inhibiting the PI3K/Akt/FoxO1 signaling pathwayJournal of AnesthesiaJune 1, 2021Haibin Cui, Qian ZhangUSA" PI3K,1:1000,ab186612,"Abcam, UK" p-PI3K,1:1000,4228S,"Cell Signaling Technology, USA" Akt,1:2000,ab32505,"Abcam, USA" p-Akt,1:2000,4051S,"Cell Signaling Technology, USA" FoxO1,1:1000,2880,"Cell Signaling Technology

(11) Silencing miRNA‑1297 suppresses the invasion and migration of prostate cancer cells via targeting modulation of PTEN and blocking of the AKT/ERK pathwayExperimental and Therapeutic MedicineMay 17, 2021Lei Wang, Jing Gao, Yu Zhang, Shaosan Kangantibody (dilution, 1:1,000; cat. no. ab38449; Abcam), anti-Akt antibody (dilution, 1:1,000; cat. no. ab32505; Abcam), anti-Erk antibody (dilution, 1:1,000; cat. no. 9102; Cell Signaling Technology, Inc.), anti-p-Erk

(12) Bcl-2 Is Involved in Cardiac Hypertrophy through PI3K-Akt PathwayBioMed Research InternationalMarch 12, 2021Xianwei Meng, Jun Cui, Guibin Heperformed as described in the literature [22]. The primary antibodies used in this study were anti-AKT (ab32505, Abcam), anti-phospho-AKT (ab81283, Abcam), anti-GSK3β (ab32391, Abcam), anti-phospho-GSK3β (ab131097,

(13) Repurposing of Pirfenidone (Anti-Pulmonary Fibrosis Drug) for Treatment of Rheumatoid ArthritisFrontiers in pharmacologyJanuary 1, 2021Gan D, Cheng W, Ke L, Sun AR, Jia Q, Chen J, Lin J, Li J, Xu Z, Zhang P.no. 7074) were purchased from CST (United States). Anti-VEGF (Cat. no. ab46154), anti-AKT (Cat. no. ab32505), and anti-MMP-3 (Cat. no. ab53015) antibodies and DAB substrate kit (Cat. no. ab64238) were obtained

(14) A Novel Long Noncoding RNA, Lnc-OAD, Is Required for Bone Morphogenetic Protein 2- (BMP-2-) Induced Osteoblast DifferentiationBioMed research internationalJanuary 1, 2021Wang Z, Zhou Y, Dai Z, Chen X, Li C, Lin Z, Wu H, Li S, Zuo C.(Thr180/Tyr182, #9215), and Runx2 (#12556) were obtained from Cell Signaling Technology (Danvers, MA USA); AKT (ab32505), p-AKT (Ser473, ab81283), and ALPL (ab108337) were purchased from Abcam (Cambridge, MA, USA); Osteocalcin

(15) Effect of Calomelanone, a Dihydrochalcone Analogue, on Human Cancer Apoptosis/Regulated Cell Death in an In Vitro ModelBioMed Research InternationalDecember 19, 2020Wasitta Rachakhom, Ratana Banjerdpongchaithe primary antibodies against Bax (ab53154), Bak (ab32371), Bcl-xL (ab98143), Atg5 (ab109490), Akt (ab32505), and β-actin (ab8227); and the secondary antibodies for goat anti-rabbit IgGhorseradish peroxidase

(16) A panel of protein kinase high expression is associated with postoperative recurrence in cholangiocarcinomaBMC CancerDecember 1, 2020Sureerat Padthaisong, Malinee Thanee, Nisana Namwat, Jutarop Phetcharaburanin, Poramate Klanrit, Narong Khuntikeo, Attapol Titapun, Watcharin LoilomeVEGF-C (1:50; # ab135506), Wnt5a (1:100; # ab72583), Beta-Catenin (1:100; # ab32572), p-Akt1 (1:100; # ab32505), Akt1 (1:50; #ab59380) were purchased from Abcam company, UK. HER2 (1:100, #4290) was purchased from

(17) miRNA-425-5p enhances lung cancer growth via the PTEN/PI3K/AKT signaling axisBMC Pulmonary MedicineDecember 1, 2020Jin-shan Zhou, Ze-shan Yang, Si-yang Cheng, Jiang-hao Yu, Chao-Jun Huang, Qiang Fengab32089; Abcam), p-AKT (dilution, 1: 8000; cat. no. ab81283; Abcam), AKT (dilution, 1: 8000; cat. no. ab32505; Abcam), p-AKT (dilution, 1: 8000; cat. no. ab81283; Abcam), ACTIN (dilution, 1: 8000; cat. no. ab8226;

(18) HGF protected against diabetic nephropathy via autophagy-lysosome pathway in podocyte by modulating PI3K/Akt-GSK3β-TFEB axisCellular signallingNovember 1, 2020Hou B, Li Y, Li X, Zhang C, Zhao Z, Chen Q, Zhang N, Li H.1995). Antibodies and reagents Antibodies against NPHS2/Podocin (ab181143), SQSTM1/p62 (ab109012), Akt (ab32505), GSK3β (ab32391), GAPDH (ab181602), rabbit IgG H&L (Alexa Fluor 488) (ab150073) and goat IgG H&L (Alexa Fluor

(19) M2 macrophages reduce the effect of gefitinib by activating AKT / mTOR in gefitinib‐resistant cell lines HCC827 / GRThoracic CancerNovember 1, 2020Fengqi Xiao, Ni Liu, Xinchun Ma, Jing Qin, Yanguo Liu, Xiuwen Wanganti‐GAPDH (Abcam, ab181602), mTOR (CST,mAb #2983), Phospho‐mTOR (Ser2448) (CST, mAb #5536). AKT (Abcam, ab32505), p‐AKT (Abcam, ab192623). Whole‐exome sequencing Whole‐exome sequencing of DNA was performed from

(20) 16-Hydroxycleroda-3,13-dien-15,16-olide Induces Apoptosis in Human Bladder Cancer Cells through Cell Cycle Arrest, Mitochondria ROS Overproduction, and Inactivation of EGFR-Related Signalling PathwaysMolecules (Basel, Switzerland)August 30, 2020Chen YC, Wang PY, Huang BM, Chen YJ, Lee WC, Chen YC.(GTX103436)",1:1000,"GeneTex (Irvine, SC, USA)" "pEGFR (Tyr1173) (ab32578), pAkt1 (Ser473) (ab81283)Akt1 (ab32505), mTOR (ab134903)",1:1000,"Abcam Plc. (Cambridge, MA, USA)" "P70S6K1 (ARG51221), EGFR (ARG66204)",1:1000,"arigo

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Anti-AKT3 + AKT2 + AKT1 antibody [Y89] from Abcam

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Anti-AKT3 + AKT2 + AKT1 antibody [Y89] from Abcam

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