Anti-BRAF antibody [EP152Y] ab33899 from Abcam

Product Specs
ItemAnti-BRAF antibody [EP152Y]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab33899
Antibody TypePrimary
IsotypeIgG
CloneEP152Y
ClonalityMonoclonal
ApplicationsImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunoprecipitation, Western blot, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunoprecipitation, Western blot, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunoprecipitation, Western blot
HostRabbit
Quantity10 UL, 40 UL, 100 UL
Conjugate/TagUnconjugated
Research AreaImmuno-oncology
Gene NameBRAF
ValidationKnockOut Validated
ReactivityHuman, Mouse, Rat
TargetBRAF

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) BRAF, TERT and HLA-G Status in the Papillary Thyroid Carcinoma: A Clinicopathological Association StudyInt J Mol SciAugust 5, 2023Bruna C. Bertol, Juliana D. Massaro, Guilherme Debortoli, André L. P. Santos, Jéssica N. G. de Araújo, Tatiana M. V. Giorgenon, Matheus Costa E Silva, Nathalie L. de Figueiredo-Feitosa, Cristhianna V. A. Collares, Luiz Carlos C. de Freitas, et al.recognizes all soluble and membrane-bound HLA-G molecules, diluted at 1:100, and (ii) the anti-BRAF EP152Y (ab33899, ABCAM, Cambridge, UK), which recognizes a peptide within the human BRAF 50-150 residues, diluted at

(2) Erianin suppresses constitutive activation of MAPK signaling pathway by inhibition of CRAF and MEK1/2Signal transduction and targeted therapyMarch 6, 2023Wang P, Jia X, Lu B, Huang H, Liu J, Liu X, Wu Q, Hu Y, Li P, Wei H, et al.Cell Signaling Technology (Danvers, MA), BRAF V600E (#A1137-25) from Biovision (San Francisco, USA), and BRAF (#ab33899), Ki67 (#ab16667) obtained from Abcam (Cambridge, USA). β-Actin (#sc47778) provided by Santa Cruz Biotechnology

(3) Whole genome sequencing delineates regulatory, copy number, and cryptic splice variants in early onset cardiomyopathynpj Genomic MedicineDecember 1, 2022Robert Lesurf et al.rabbit polyclonal TGFβ3 antibody (ab15537, Abcam, Cambridge, UK), rabbit monoclonal BRAF antibody (ab33899, Abcam, Cambridge, UK) or NRAP polyclonal antibody (PAS-88772; Invitrogen, Carlsbad, California, USA)

(4) PPP1R14D promotes the proliferation, migration and invasion of lung adenocarcinoma via the PKCÎ±/BRAF/MEK/ERK signaling pathwayInternational Journal of OncologyOctober 19, 2022Huijun Cao, Zhiqiang Wang, Ying Wang, Lijuan Ye, Ruilei Li, Yuanbo Xue, Ke Li, Tiannan Di, Tao Li, Zonglin Fan, et al.ab92547), Kras (1:1,000; cat. no. ab275876), PKCα (1:1,000; cat. no. ab32376), BRAF (1:1,000; cat. no. ab33899), MEK1 (1:2,000; cat. no. ab32576), and phosphorylated (p)-MEK1 (1:1,000; cat. no. ab32088; all from

(5) Identification of a BRAF/PA28γ/MEK1 signaling axis and its role in epithelial-mesenchymal transition in oral submucous fibrosisCell death & diseaseAugust 12, 2022Xie C, Li Z, Hua Y, Sun S, Zhong L, Chen Q, Feng H, Ji N, Li T, Zhou X, et al.for western blot analysis are listed below. PA28γ (PA5-21789, Invitrogen, Carlsbad, USA), 1:2000. BRAF (ab33899, Abcam, Cambridge, UK), 1:2000. MEK1 (ab32091, Abcam, Cambridge, UK), 1:2000. p-MEK1 (ab96379, Abcam,

(6) Correlation of LAGE3 with unfavorable prognosis and promoting tumor development in HCC via PI3K/AKT/mTOR and Ras/RAF/MAPK pathwaysBMC CancerMarch 21, 2022Yun Li, Hui Xiong2352,1:1000,45 p-Erk,CST 4376,1: 1000,"42, 44" Erk (MAPK1),CST 4695,1: 1000,"42, 44" Braf,Abcam ab33899,1: 2000,90 GAPDH,Abcam ab181602,1: 10000,36 The information of primary antibodies in Western Blotting

(7) The Oncogenic Signaling Pathways in BRAF-Mutant Melanoma Cells are Modulated by Naphthalene Diimide-Like G-Quadruplex LigandsCellsOctober 18, 2019Marta Recagni, Martina Tassinari, Filippo Doria, Graziella Cimino-Reale, Nadia Zaffaroni, Mauro Freccero, Marco Folini, Sara N. RichterCambridge, United Kingdom); rabbit polyclonal anti-AKT(S473) (sc-9271, Santa Cruz Biotechnology), anti-BRAF (ab33899, Abcam,), anti-ERK1/2 (T202/Y204) (#9101, Cell Signaling Technology), anti-γ-H2AX (ab11174, Abcam),

(8) Anticancer effects of combinational treatment with BRAFV600E siRNA and PI3K pathway inhibitors in melanoma cell lines harboring BRAFV600EOncology LettersMay 2, 2018Hongyan He, Xiyan Nan, Shuang Liu, Liangren Zhang, Zhenjun Yang, Yun Wu, Lihe Zhangand methods Reagents. Antibodies used in the present study were as follows: Anti‑BRAF (catalog no., Ab33899; Abcam, Cambridge, UK); anti‑MEK1 (catalog no., 9146), anti‑MEK2 (catalog no.,), anti‑ERK1/2 (catalog

(9) MicroRNA‑195 is associated with regulating the pathophysiologic process of human laryngeal squamous cell carcinomaMolecular medicine reportsApril 1, 2018Pang H, Xu X, Dai L, Wang K, Yao X.primary antibodies: VEGFR2 (9698S, 1:1,000; Cell Signaling Technology, Inc., Danvers, MA, USA), Raf (ab33899, 1:1,000; Abcam, Cambridge, UK), extracellular signal‑regulated kinase (ERK, sc‑514302, 1:1,000), phosphorylated

(10) Electroacupuncture serum inhibits TNF‑α‑mediated chondrocyte inflammation via the Ras‑Raf‑MEK1/2‑ERK1/2 signaling pathwayMolecular medicine reportsNovember 1, 2017Chen H, Shao X, Li L, Zheng C, Xu X, Hong X, Li X, Wu M.The membranes were then incubated with rat monoclonal antibodies against Ras (ab52939; 1:5,000), Raf (ab33899; 1:2,000), MEK1/2 (ab178876; 1:5,000) and β-actin (ab6276; 1:1,000) (Abcam, Cambridge, UK), and ERK1/2

(11) Targeting of the MAPK and AKT pathways in conjunctival melanoma shows potential synergyOncotargetAugust 29, 2017Jinfeng Cao, Renier C. Heijkants, Aart G. Jochemsen, Mehmet Dogrusöz, Mark J. de Lange, Pieter A. van der Velden, Sjoerd H. van der Burg, Martine J. Jager, Robert M. VerdijkUSA) and exposed to X-ray films (Fuji Super RX, Japan). Antibodies are listed below: BRAF (1:1000; ab33899, Abcam, Cambridge, UK); anti-MAP kinase diphosphorylated ERK 1/2 (1:1000; M8159, Sigma-Aldrich); p44/42

(12) Metformin ameliorates core deficits in a mouse model of fragile X syndromeNature medicineJune 1, 2017Gantois I, Khoutorsky A, Popic J, Aguilar-Valles A, Freemantle E, Cao R, Sharma V, Pooters T, Nagpal A, Skalecka A, et al.Cell 349 Signaling); phospho-Raptor (2083, Cell Signaling); c-Raf (53745, Cell Signaling); b-Raf 350 (ab33899, Abcam); phospho-b-Raf S729 (ab124794, Abcam); phospho-b-Raf S602 (PA5- 351 38412, Thermo Fisher Scientific);

(13) UPLC-PDA-QTOFMS-guided isolation of prenylated xanthones and benzoylphloroglucinols from the leaves of Garcinia oblongifolia and their migration-inhibitory activityScientific ReportsDecember 21, 2016Hong Zhang, Zheng Zheng, Zhi-Jie Ding, Yuan-Zhi Lao, Hong-Sheng Tan, Hong-Xi Xuwere purchased from Cell Signaling Technologies (Danvers, MA, USA). RAS (Cat.ab137739) and B-RAF (Cat.ab33899) were purchased from Abcam (Cambridge, UK). Additional Information How to cite this article: Zhang,

(14) P2A-Fluorophore Tagging of BRAF Tightly Links Expression to Fluorescence In VivoPLOS ONEJune 27, 2016J. Edward van Veen, Daphne R. Pringle, Martin McMahonresuspended in PBT containing primary antibodies used at the following concentrations: Anti-BRAF (Abcam, ab33899) 1:25; Anti-DSRed (Clontech, 632496) 1:200. Cells were then pelleted by centrifugation, washed with

(15) Lauric acid can improve the sensitization of Cetuximab in KRAS/BRAF mutated colorectal cancer cells by retrievable microRNA-378 expressionOncology reportsJanuary 1, 2016Weng WH, Leung WH, Pang YJ, Hsu HH.dilution of anti-KRAS (mouse monoclonal, ab55391), 1:2,500 dilution of anti-BRAF (rabbit monoclonal, ab33899), 1:2,000 dilution of anti-MEK (mouse monoclonal, ab69502), 1:2,500 dilution of anti-ERK (mouse monoclonal,

(16) Griffipavixanthone, a dimeric xanthone extracted from edible plants, inhibits tumor metastasis and proliferation via downregulation of the RAF pathway in esophageal cancerOncotargetDecember 7, 2015Zhijie Ding, Yuanzhi Lao, Hong Zhang, Wenwei Fu, Lunlun Zhu, Hongsheng Tan, Hongxi Xuwere purchased from Cell Signaling Technologies (Danvers, MA, USA). RAS (Cat.ab137739) and B-RAF (Cat.ab33899) were purchased from Abcam (Cambridge, UK). Colony formation assay For the colony formation assay,

(17) Common Oncogene Mutations and Novel SND1-BRAF Transcript Fusion in Lung Adenocarcinoma from Never SmokersScientific reportsMay 18, 2015Jang JS, Lee A, Li J, Liyanage H, Yang Y, Guo L, Asmann YW, Li PW, Erickson-Johnson M, Sakai Y, et al.were blocked with 5% skim milk in TBS-Tween-20 and incubated with the diluted at 1:3000 anti-BRAF (#ab33899, Abcam), anti-MEK (#4694, Cell Signaling), anti-pMEK(#2338, Cell Signaling), anti-ERK (#4370, Cell Signaling),

(18) B-Raf regulation of integrin α4β1-mediated resistance to shear stress through changes in cell spreading and cytoskeletal association in T cellsThe Journal of biological chemistryAugust 15, 2014Brown WS, Khalili JS, Rodriguez-Cruz TG, Lizee G, McIntyre BW.

(19) Ekspresi Protein B-RAF Mutan pada Karsinoma Tiroid Papilifer yang Bermetastasis ke Kelenjar Getah Bening RegionalMajalah Kedokteran BandungDecember 1, 2013Dimyati Achmad, Jeremy Sebastian, Bethy S. Hernowo, Kiki A. Rizkitemperatur ruangan). Untuk menentukan ekspresi B-RAF mutan digunakan antibodi Rabbit monoclonal (EP 152 Y) ab33899 dari Abcam, United Kingdom. Hasil pemeriksaan variabel bebas dalam skala pengukuran semikuantitatif

(20) Alternative splicing of BRAF transcripts and characterization of C-terminally truncated B-Raf isoforms in colorectal cancerInternational journal of cancerAugust 1, 2013Benjamin Hirschi, Frank T Kolligs

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2/14/2017Excellent Antibody For Detection Of Human BRAF Protein Fabrice JaffreThe aim of the study was to detect BRAF protein expression in human iPSC-derived cardiomyocytes by Western Blot.

Anti-BRAF antibody [EP152Y] from Abcam

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Anti-BRAF antibody [EP152Y] from Abcam

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