Fig 1: E. coli induced neutrophil infiltration in the fetal membranes.A. Immunohistochemistry analysis of mice fetal membranes collected from 6 and 24 hr after 1011 CFU of E. coli vaginal administration (N = 3). Neutrophil infiltration detected at 24 hr after the bacterial infection in the fetal membrane tissues (white arrows pointing to Ly-6G positive cells). However, 6 hr after bacterial infection, neutrophil detection was comparable to non-infected controls. (Scale bar, 100 µm). B. Quantification of neutrophils in fetal membrane in (A). Number of Ly-6G positive cells per random fields (N = 3). The data are presented as means ± SEM.* P = 0.0219, paired t-test.