Fig 1: Increased numbers of ILCs in blood of psoriatic patients with metabolic disorders. (A) Gating strategy to define subpopulations of innate lymphoid cells ILC1, ILC2, ILC3. Analysis was performed by flow cytometry by using lineage Cocktail-Pacific Blue (Biolegend), CD11c-BV421, CD34-BV421, CD94-BV421, FcERIa-BV421, CD45-VioGreen, CD117-PE (c-Kit), IL7RA-APC (CD127), CRTH2-APC-Cy7 (CD294). ILCs are pregated as CD45 positive cells that are further defined by negative expression of the lineage cocktail and positive staining for IL7RA ( Supplementary Figure 2 ). The figure is presenting cells covering these criteria and their further subdivision by individual expression of surface antigens CD117 and CRTH2. ILC1 are blue bordered (CD117-, CRTH2-), ILC2 red (CRTH2+) and ILC3 orange (CD117+, CRTH2-). Total ILCs are colored in green. (B) Number of ILCs of healthy controls (unfilled dots) and patients with psoriasis vulgaris (filled dots) in blood, mean and SD, each dot represents an individual patient, Mann-Whitney test. (C) Number of ILCs in blood of normal-weight psoriatic patients (body mass index, BMI<30 kg/m2; unfilled triangle; n=8) and obese patients (BMI =30 kg/m2; filled triangle; n=8) are shown, mean and SD, Mann-Whitney test. (D) Number of ILCs in blood of psoriatic patients without atherosclerosis (unfilled trapezoid; n= 9) and with atherosclerosis (filled trapezoid; n=7) are shown, mean and SD, Mann-Whitney test. (E) Number of ILCs of psoriatic patients without hepatic remodeling (cross symbol; n=7) and patients with steatosis hepatis (star; n=9), mean and SD, Mann-Whitney test. (F) Linear correlation of the number of circulating ILC and BMI (n=15), Pearson correlation. (G) Linear correlation of the number of circulating ILC1 and PROCAM score [%] (n=16), Pearson correlation. *p < 0.05; **p < 0.01; ***p < 0.001. ns, not significant.