Fig 1: Evaluation of rabbit IPFSCs' proliferation and chondrogenic capacity after expansion on dECMs deposited by human SDSCs (SECM), DFs (DECM), ADSCs (AECM), and UDSCs (UECM). The Plastic group serves as a control culture substrate. (A) Human UDSC cell morphology. (B) Rabbit IPFSCs' morphology after expansion on C-dECMs and Plastic. Cell proliferation was evaluated using relative EdU incorporation (C) and combination with cell growth (D), as well as CD146 surface marker expression (E) after expansion on C-dECMs and Plastic. (F) The size of tissue constructs (expanded cells + PLGA scaffolds) was measured at Days 0, 10, and 21 following chondrogenic induction. (G) Expanded cells (fold change adjusted by the data collected from the Plastic group) and Day 10 tissue constructs (fold change adjusted by the data collected from the corresponding cell samples) were used to evaluate chondrogenic marker gene expression including SOX9, COL2A1, ACAN, COL1A1, and COL10A1. Data are presented as average ± SD for n = 3. In each comparison, groups not connected by the same letter are significantly different (p < 0.05). (H) Representative tissue constructs were shown following 10-day chondrogenic induction.