Fig 1: SMYD3 mediates the HR pathway. (a) Relative HR activity of shLuc, shSMYD3, and shEXO1 MCF7 cells, which was indicated by the percentage of GFP-positive cells relative to the shLuc control. The EXO1 knockdown cells were used as a positive control. ***P < 0.001 vs. shLuc control. (b) Relative NHEJ activity of shLuc, shSMYD3 and shKu70 cells. NHEJ activity was measured by normalizing the luciferase activity to the renilla activity. The Ku70-knockdown cells were used as a positive control. ***P < 0.001 vs. shLuc control. (c) Relative MMEJ activity of shLuc, shSMYD3 and shPOLQ MCF7 cells, which was indicated by the percentage of GFP-positive cells relative to the shLuc control. The POLQ-knockdown cells were used as a positive control. ***P < 0.001 vs. shLuc control. (d) Exogenous expression of SMYD3, but not the mutant SMYD3Y239F, restored the HR activity. ***P < 0.01 vs. shLuc with the expression of the vector plasmid control. N.S.: not significant.