Nitrotyrosine Antibody MAB3248 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemNitrotyrosine Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberMAB3248
ApplicationsWestern Blot, Immunocytochemistry
Quantity100 ug (also 25 ug)
TargetNitrotyrosine
Clone306507
IsotypeIgG3
ReactivityMulti-Species
HostMouse
Conjugate/TagUnconjugated
ClonalityMonoclonal
FormatLyophilized from a 0.2 µm filtered solution in TBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ImmunogenNitrotyrosine-modified KLH
ConcentrationLYOPH
PurityProtein A or G purified from hybridoma culture supernatant
Brand/TrademarkR&D Systems, a Bio-Techne Brand
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Unconjugated

Specificity: Detects Nitrotyrosine adducts on proteins in Western blots. It does not cross-react with phosphotyrosine or 4-hydroxynonenal adducts. Unfixed cells, tissues, and proteins can be treated with Peroxynitrite (Catalog # AR006 ) for use as positive controls with this antibody

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Multi-omic screening of invasive GBM cells in engineered biomaterials and patient biopsies reveals targetable transsulfuration pathway alterationsbioRxivFebruary 28, 2023Joseph H. Garcia, Erin A. Akins, Saket Jain, Kayla J. Wolf, Jason Zhang, Nikita Choudhary, Meeki Lad, Poojan Shukla, Sabraj Gill, Will Carson, et al.hydrogel devices and patient tumors. Samples were probed with mouse anti-Nitrotyrosine (R&D Systems, MAB3248, 1:20) or mouse anti-Malondialdehyde (Thermo Fisher Sci, MA527559, 1:100) and later probed with goat

(2) Inflammation Promotes Oxidative and Nitrosative Stress in Chronic Myelogenous LeukemiaBiomoleculesFebruary 3, 2022Đikić D, Bogdanović A, Marković D, Mitrović-Ajtić O, Subotički T, Diklić M, Vukotić M, Dragojević T, Živković E, Santibanez JF, et al.

(3) Cellular Response against Oxidative Stress, a Novel Insight into Lupus Nephritis PathogenesisJournal of personalized medicineJuly 22, 2021Ene CD, Georgescu SR, Tampa M, Matei C, Mitran CI, Mitran MI, Penescu MN, Nicolae I.between 78 and 5000 pg/mL. Circulant 3-NT was determined by ELISA method using the R&D systems reactive (MAB3248 kit) and a TECAN analyzer (Tecan, Switzerland). Carbonyl groups were assessed by spectrophotometric

(4) Posttranslational Modifications Pattern in Clear Cell Renal Cell CarcinomaMetabolitesDecember 27, 2020Ene CD, Penescu MN, Georgescu SR, Tampa M, Nicolae I.from proteins. Circulant 3-NT was assessed immunoenzymatically by ELISA using the R&D systems reactive (MAB3248 kit) and a TECAN analyzer (Tecan, Switzerland). Carbonyl groups, as result of oxidative degradation

(5) Sex-dimorphic moderate hypoglycemia preconditioning effects on Hippocampal CA1 neuron bio-energetic and anti-oxidant functionMolecular and cellular biochemistryOctober 1, 2020Uddin MM, Ibrahim MMH, Aryal D, Briski KP.ProSci.), ATPs (CA-42704, 1:1500; ProSci.), 4-HNE (NB100–63093, 1:3000, Novus Biol.), or mouse [NT (MAB3248; 1:2000; R&D Research, MN)], Membranes were incubated for 1 hr with peroxidase-conjugated rabbit anti-goat

(6) Nitrosative Stress in the Frontal Cortex From Dogs With Canine Cognitive DysfunctionFrontiers in veterinary scienceJanuary 1, 2020Prpar Mihevc S, Zakošek Pipan M, Štrbenc M, Rogelj B, Majdič G.Novus Biologicals" 3-NT,Rabbit polyclonal,1:200,"06-284, Millipore" 3-NT,Mouse monoclonal,1:200,"MAB3248, R&D Systems" TUBB3,Mouse monoclonal,1:50,"sc-80005, Santa Cruz Biotechnology" NEUN,Mouse monoclonal,1:200,"MAB377,

(7) Deleterious effects of phosphate on vascular and endothelial function via disruption to the nitric oxide pathwayNephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal AssociationOctober 1, 2017Stevens KK, Denby L, Patel RK, Mark PB, Kettlewell S, Smith GL, Clancy MJ, Delles C, Jardine AG.

(8) Apolipoprotein D modulates amyloid pathology in APP/PS1 Alzheimer's disease miceNeurobiology of AgingMay 1, 2015Hongyun Li et al.HNE (recognizes proteins modified by the aldehydic lipid peroxidation end product, 4-hydroxynonenal) (MAB3248, R&D Systems, 1:2,000) and β-actin (A5060, Sigma, 1:10,000). Signals were detected using species-specific HRP-conjugated secondary 11 antibodies (Dako) and enhanced chemiluminescence

(9) Phytoestrogen Î±-Zearalanol attenuates homocysteine-induced apoptosis in human umbilical vein endothelial cellsBioMed Research InternationalOctober 1, 2013Teng Liu, Dan-dan Hou, Qian Zhao, Wei Liu, Pan-pan Zhen, Jian-ping Xu, Ke Wang, Hai-xia Huang, Xiao Li, Hui Zhang, et al.antibody (C9) were procured from Cell Signaling Technology (Danvers, MA, USA). Anti-3-nitrotyrosine (MAB3248) was purchased from R&D Systems (Minneapolis, MN, USA). Rabbit anti-NOS3 (endothelial nitric oxide synthase,

(10) Age-related retinopathy in NRF2-deficient micePloS oneApril 29, 2011Zhao Z, Chen Y, Wang J, Sternberg P, Freeman ML, Grossniklaus HE, Cai J.

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Nitrotyrosine Antibody

Nitrotyrosine Antibody

Nitrotyrosine Antibody

Nitrotyrosine Antibody

Nitrotyrosine Antibody

Nitrotyrosine Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (10)

Reviews

Nitrotyrosine Antibody from R&D Systems, a Bio-Techne Brand

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