Fig 1: Confirmation of APOA1, APOA4, EIF4G1, and AZGP1 expressions by Western blot analysis. All peritoneal dialysate samples of CGN and DM patients were confirmed by Western blot analysis and the representatives are shown in this figure.
Fig 2: Secretome analysis in sera and MAC cultureMass spectroscopy analysis exclusively detected APN A. or AZGP1 B. expression only in the breast cancer patients with nontuberculous Mycobacterium (BCa-NTMnb) infection. The spectra (panel 2) and 2 unique peptide sequences of APN (a, shaded yellow, panel 3) and AZGP1 (b, yellow, panel 3) was detected in the exosomes. C. ECM1 expression was not detectable in the MAC culture derived exosomes of NTMnb cases (N=7) but detectable in all BCa-NTMnb subjects (N=10). Human breast cancer cell lines MDA-MB-231, SUM-149 and one BCa-NTMnb subject were used as positive control for ECM1 detection. One healthy and one NTMnb subject were used as negative controls. Syntenin was used as an exosome marker and loading control. D. Model suggesting the possible role of ECM1, APOC4, APN and AZGP1. Exosome derived ECM1 in concert with other secretory and lypolysis associated proteins APOC4, APN and AZGP1 might have influenced BCa development and immune suppression leading to increased NTM susceptibility.
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