Fig 1: PLOD3 expression in CRC and normal tissues from the GSE74602 (A) and GSE113513 (B) cohorts; PLOD3 protein expression difference in CRC and normal tissues and TMT ratio means the protein abundance by TMT-based quantitation (C) and ROC curve of PLOD3 protein for discriminating tumor and normal tissues in proteome dataset (D); (E) Representative microphotographs of PLOD3 immunohistochemical staining in CRC tissue and adjacent normal tissue by IHC. Scale bar = 50 µm. (F) IHC scores in CRC tissues and adjacent tissues. Prognostic value of PLOD3 in the GSE17536 (G) and GSE39582 (H) cohorts; Prognostic value of PLOD3 in the proteome dataset (I, J).
Fig 2: Pan-cancer PLOD3 expression analysis. (A) PLOD3 expression in tumor and normal tissues from pan-cancer data of The Cancer Genome Atlas (TCGA). (B) PLOD3 expression in tumor and normal tissues from CRC obtained from TCGA. (C) PLOD3 expression in paired CRC tumor and normal tissues from TCGA. Data are expressed as the mean ± SD. **p < 0.01, ***p < 0.001, ****p < 0.0001.
Fig 3: Minoxidil inhibited the expression of LHs, collagens, and a-SMA. The protein expression was analyzed by Western blotting. (A) The LH1, LH2, and LH3 protein expression in the lungs of mice on day 21 following BLM administration; (B) The COL I, COL IV and a-SMA protein expression in the lungs of mice on day 21 following BLM administration. The relative expression levels in lung tissue samples were normalized to the level of ß-actin (Actin). Values are given as the means ±SD, n=6. * P<0.05 vs. the NS group, ** P<0.01 vs. the NS group, *** P<0.001 vs. the NS group; # P<0.05 vs. the BLM group, ## P<0.01 vs. the BLM group.
Fig 4: The PLOD expression in human subjects. The expression levels of PLOD1, PLOD2, and PLOD3 mRNA in human serum were determined by qRT-PCR. Data are presented as the means ±SD, n=15, ** P<0.01 vs. the Control group, *** P<0.001 vs. the Control group.
Fig 5: Immunohistochemical staining for COL1 and the LH proteins. The expression of the COLI (A), LH1 (B), LH2 (C), and LH3 (D) proteins. Representative images are shown. All images are 200× magnification.
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