α Tubulin (4G1) Antibody sc-58666 from Santa Cruz Biotechnology, Inc.

Product Specs
Itemα Tubulin (4G1) Antibody
CompanySanta Cruz Biotechnology, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog Numbersc-58666
Antibody TypePrimary Antibodies
NCBI Gene AliasesTuba1, TUBA1, H2-ALPHA, Tuba-1, ALS22
Clone4G1
Quantity100 µg/ml
Reactivitymouse, rat, human
ApplicationsWB, IP, IF, IHC(P)
Conjugate/TagUnconjugated
ClonalityMonoclonal
FormatPurified
Hostmouse
NCBI Full Gene Nametubulin, alpha 1A, tubulin alpha 4a
Targetalpha Tubulin
IsotypeIgG1

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Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) NCoA3 upregulation in breast cancer‑associated adipocytes elicits an inflammatory profileOncology ReportsApril 3, 2023María Cecilia Lira, Francisco D. Rosa, Ignacio Aiello, Mileni Soares Machado, Alejandra G. Palma, Leonardo Paz, María Cecilia Salazar Güemes, Silvia Burlando, Pablo J. Azurmendi, Natalia Paladino, et al.polyclonal anti-NCoA3 (1:4,000; cat. no. sc-25742) or mouse monoclonal anti-tubulin (1:5,000; cat. no. sc-58666) antibodies (Santa Cruz Biotechnology, Inc.) overnight at 4°C in TBS-0.02%Tween containing 0.5% bovine

(2) A novel diG motif in ORF3a protein of SARS-Cov-2 for intracellular transportFrontiers in Cell and Developmental BiologyNovember 23, 2022Ruth Cruz-Cosme, Jiantao Zhang, Dongxiao Liu, Vidhyanand Mahase, Bhargava Teja Sallapalli, Peixi Chang, Yanjin Zhang, Shaolei Teng, Richard Y. Zhao, Qiyi Tangaccording to the manufacturer’s protocol. Antibodies and reagents Mouse antibodies against Tubulin (4G1, sc-58666), late endosomes (Rab7, B-3, sc-376362), and LAMP1 (E-5, sc-17768) were purchased from Santa Cruz Biotechnology

(3) CRISPR‐Cas9 –Mediated Genome Editing Confirms EPDR1 as an Effector Gene at the BMD GWAS ‐Implicated ‘ STARD3NL ’ LocusJBMR PlusSeptember 1, 2021James A Pippin, Alessandra Chesi, Yadav Wagley, Chun Su, Matthew C Pahl, Kenyaita M Hodge, Matthew E Johnson, Andrew D Wells, Kurt D Hankenson, Struan F A Grantdry milk and incubated overnight at 4°C with EPDR1 antibody (ab197932; Abcam) and α‐tubulin antibody (sc‐58666; Santa Cruz Biotechnology). On the following day, membranes were washed, incubated with horseradish‐peroxidase–linked

(4) Increased S1P expression in osteoclasts enhances bone formation in an animal model of Paget's diseaseJournal of cellular biochemistryApril 1, 2021Nagata Y, Miyagawa K, Ohata Y, Petrusca DN, Pagnotti GM, Mohammad KS, Guise TA, Windle JJ, David Roodman G, Kurihara N.(sc‐10758), NFATc‐1 (sc‐7294, clone 7A6), EDG‐3 target for S1PR3 (sc‐30024, clone [H‐70]), and α‐tubulin (sc‐58666, clone4G1) antibodies were from Santa Cruz. Anti‐osterix antibody (ab22552) was purchased from Abcam

(5) GPR75 receptor mediates 20-HETE-signaling and metastatic features of androgen-insensitive prostate cancer cellsBiochimica et biophysica acta. Molecular and cell biology of lipidsFebruary 1, 2020Cárdenas S, Colombero C, Panelo L, Dakarapu R, Falck JR, Costas MA, Nowicki S.immunofluorescence assays, rhodamine conjugated phalloidin (#P1951, 1/200, Sigma-Aldrich) or anti α-tubulin (ID#sc58666, 1/200, Santa Cruz Biotechnology) were used. 4′,6′-diamidino-2-phenylindole (DAPI) was from Thermo Fisher

(6) The SIRT2-mediated deacetylation of AKR1C1 is required for suppressing its pro-metastasis function in Non-Small Cell Lung CancerTheranosticsJanuary 1, 2020Zhu H, Hu Y, Zeng C, Chang L, Ge F, Wang W, Yan F, Zhao Q, Cao J, Ying M, et al.db2603), GAPDH (Diag Biotechnology, db1209), β-Actin (Santa Cruz, sc-1615), α-tubulin (Santa Cruz, sc-58666), Flag (Genescript, A00187-100), Sox2 (Santa Cruz, sc-365964), Vimentin (Santa Cruz, sc-80975). In Vitro

(7) Sirtuin 6 Attenuates Kaposi's Sarcoma-Associated Herpesvirus Reactivation by Suppressing Ori-Lyt Activity and Expression of RTAJournal of VirologyApril 1, 2019Min Hu, Najealicka Armstrong, Edward Seto, Wenwei Li, Fanxiu Zhu, Paul C. Wang, Qiyi Tang(M2) were purchased from Sigma-Aldrich (St. Louis, MO). The monoclonal antibodies against tubulin (sc-58666) and GFP (sc-9996) were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Anti-RTA monoclonal

(8) Spectrin is a mechanoresponsive protein shaping fusogenic synapse architecture during myoblast fusionNature Cell BiologyJune 1, 2018Rui Duan et al.sc-377460), Myogenin (1:100; sc-52903), skeletal muscle myosin (1:100; sc-32732) and α-tubulin (1:100; sc-58666) from Santa Cruz Biotechnology. Protein quantification was performed using the photoshop image software

(9) PPARγ deacetylation dissociates thiazolidinedione's metabolic benefits from its adverse effectsThe Journal of clinical investigationJune 1, 2018Kraakman MJ, Liu Q, Postigo-Fernandez J, Ji R, Kon N, Larrea D, Namwanje M, Fan L, Chan M, Area-Gomez E, et al.anti-perilipin (Cell Signaling Technology, catalog 9349), anti-tubulin (Santa Cruz Biotechnology Inc., catalog sc-58666), anti-actin (Cell Signaling Technology, catalog 8457), and anti-Ucp1 (Abcam, catalog 10983). SVF isolation.

(10) Transcriptional coactivator PGC-1α contains a novel CBP80-binding motif that orchestrates efficient target gene expressionGenes & developmentApril 1, 2018Cho H, Rambout X, Gleghorn ML, Nguyen PQT, Phipps CR, Miyoshi K, Myers JR, Kataoka N, Fasan R, Maquat LE.(Abcam, ab21060), anti-eIF4E (Bethyl Laboratories, A301-153A), anti-α-Tubulin (Santa Cruz Biotechnology, sc-58666), anti-P62 (BabCO, mAb414), anti-vimentin (Santa Cruz Biotechnology, sc-6260), anti-pSer2-RNAPII (Abcam,

(11) Molecular cloning and characterization of the genes encoding the proteins of Zika virusGeneSeptember 10, 2017Wangheng Hou, Ruth Cruz-cosme, Najealicka Armstrong, Lilian Akello Obwolo, Fayuan Wen, Wenhui Hu, Min-Hua Luo, Qiyi TangLC3beta (sc-271625) to visualizing Autophgosomes, anti-Lamin A (h-102, sc-20680), and anti-Tubulin (4G1, sc-58666) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). The anti-ZIKV antibody for immunocytochemistry

(12) BAP1 regulates IP3R3-mediated Ca²⁺ flux to mitochondria suppressing cell transformationNatureJune 22, 2017Bononi A, Giorgi C, Patergnani S, Larson D, Verbruggen K, Tanji M, Pellegrini L, Signorato V, Olivetto F, Pastorino S, et al.procedures. Antibodies Primary antibodies used were: α-Tubulin (4G1) (Santa Cruz Biotechnology, cat. no. sc-58666), BAP1 (C-4) (Santa Cruz Biotechnology, cat. no. sc-28383), BAP1 (D7W7O) (Cell Signaling, cat. no. 13271),

(13) Endothelial Mesenchymal Transition: Comparative Analysis of Different Induction MethodsBiological Procedures OnlineDecember 1, 2016Mariana T. Pinto, Dimas T. Covas, Simone Kashima, Claudia O. Rodriguesconfirmed by Ponceau Staining and probing with endogenous control antibodies anti-α-Tubulin (Santa Cruz, SC-58666) and anti-Gapdh (Cell Signaling, #2118). The same blot was probed with different antibodies without

(14) Glycogen synthase kinase 3β ubiquitination by TRAF6 regulates TLR3-mediated pro-inflammatory cytokine productionNature communicationsApril 1, 2015Ko R, Park JH, Ha H, Choi Y, Lee SY.dilution 1:1,000), ubiquitin (sc-8017, dilution 1:1,000), β-actin (sc-87778, dilution 1:1,000), α-tubulin (sc-58666, dilution 1:1,000) and GAPDH (sc-87724, dilution 1:1,000) were from Santa Cruz Biotechnology. A GSK3α/β-specific

(15) Arsenic Trioxide Reduces Global Histone H4 Acetylation at Lysine 16 through Direct Binding to Histone Acetyltransferase hMOF in Human CellsPloS oneJanuary 1, 2015Liu D, Wu D, Zhao L, Yang Y, Ding J, Dong L, Hu L, Wang F, Zhao X, Cai Y, et al.Montgomery, TX). Anti-HDAC4 (17449-1-AP) polyclonal antibodies were from Boster (China, Wuhan). Antitubulin (sc-58666) was acquired from Santa Cruz (Dallas, TX). Anti-GAPDH (NM_002046) rabbit polyclonal antibodies were

(16) Negative Regulation of p21Waf1/Cip1 by Human INO80 Chromatin Remodeling Complex Is Implicated in Cell Cycle Phase G2/M Arrest and Abnormal Chromosome StabilityPloS oneJanuary 1, 2015Cao L, Ding J, Dong L, Zhao J, Su J, Wang L, Sui Y, Zhao T, Wang F, Jin J, et al.Methods Antibodies Anti-YY1 (H414, sc-1703X) rabbit polyclonal antibody, anti-Myc (9E10) and anti-α-tubulin (sc-58666) monoclonal antibodies, rabbit total IgG (sc-2027) and mouse total IgG (sc-2025) were obtained from

(17) Characterization of M. tuberculosis SerB2, an Essential HAD-Family Phosphatase, Reveals Novel PropertiesPLoS ONEDecember 18, 2014Gaya Prasad Yadav, Sonal Shree, Ruchi Maurya, Niyati Rai, Diwakar Kumar Singh, Kishore Kumar Srivastava, Ravishankar Ramachandranincomplete medium and fixed with ice-cold methanol. Cells were further incubated with α-Tubulin (4G1: sc-58666, Santa Cruz inc.) antibody at 1∶1000 dilution for 1 h at 37°C, followed by secondary antibody goat anti-mouse

(18) Novel transcriptional regulation of VEGF in inflammatory processesJournal of Cellular and Molecular MedicineFebruary 18, 2013Xiaoren Tang, Yu Yang, Huaiping Yuan, Jian You, Marina Burkatovskaya, Salomon AmarInc., Lewisville, TX, USA), TBP (sc-34862; Santa Cruz), or actin (sc-1615; Santa Cruz) and tubulin (sc-58666; Santa Cruz) as control for Western blot analysis. siRNAs The sequences of siRNA were designed by

(19) Nitric oxide produced by cytochrome c oxidase helps stabilize HIF-1α in hypoxic mammalian cellsBiochemical and biophysical research communicationsApril 20, 2012Ball KA, Nelson AW, Foster DG, Poyton RO.following antibodies were used: anti-HIF-1α (BD-Bioscience 610958 at 1:1000); anti-tubulin (Santa Cruz sc-58666 at 1:2,000 or NeoMarkers at 1:8000); anti-Histone H2B (Cell Signaling 2934S at 1:30,000); and anti-mouse

(20) Characterization of Basigin Isoforms and the Inhibitory Function of Basigin-3 in Human Hepatocellular Carcinoma Proliferation and InvasionMolecular and Cellular BiologyJuly 1, 2011Cheng-Gong Liao et al.against green fluorescent protein (GFP) (sc-9996), MMP-2 (sc-10736), MMP-9 (sc-10737), and ␣-tubulin (sc-58666) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Proliferating cell nuclear antigen (PCNA)

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α Tubulin (4G1) Antibody from Santa Cruz Biotechnology, Inc.

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Santa Cruz Biotechnology, Inc.

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α Tubulin (4G1) Antibody from Santa Cruz Biotechnology, Inc.

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