Fig 2: Histological results of hepatocellular carcinoma tumors treated with U, anti-alpha-fetoprotein-ultra-small superparamagnetic iron oxide, anti-glypican-3-ultra-small superparamagnetic iron oxide, and anti-alpha-fetoprotein-ultra-small superparamagnetic iron oxide-anti-glypican-3 probes. UA: Anti-AFP-USPIO; UG: Anti-GPC3-USPIO; UAG: Anti-AFP-USPIO-anti-GPC3; USPIO: Ultra-small superparamagnetic iron oxide; AFP: Alpha-fetoprotein; GPC3: Glypican-3.

Fig 3: Experiment workflow. Probe treatment and T1- and T2-weighted magnetic resonance imaging were performed on day 10 after establishment of the orthotopic hepatocellular carcinoma mouse model. MRI: Magnetic resonance imaging; HCC: hepatocellular carcinoma; UA: Anti-AFP-USPIO; UG: Anti-GPC3-USPIO; UAG: Anti-AFP-USPIO-anti-GPC3.

Fig 4: T1-weighted images of hepatocellular carcinoma-model mice administered with ultra-small superparamagnetic iron oxide probes at various time points. Columns represent livers treated with different probes, and the first row shows pre-injection (0 h) T2-weighted images as references for the tumor location indicated by yellow arrowhead. UA: Anti-AFP-USPIO; UG: Anti-GPC3-USPIO; UAG: Anti-AFP-USPIO-anti-GPC3.

Fig 5: T2-weighted images of hepatocellular carcinoma-model mice administered ultra-small superparamagnetic iron oxide probes at various time points. Columns represent livers treated with different probes. (Yellow arrowhead: tumor location). UA: Anti-AFP-USPIO; UG: Anti-GPC3-USPIO; UAG: Anti-AFP-USPIO-anti-GPC3.