antibody dilution: 1 : 1000 sample: total cell lysate from fibroblasts transfected with a GFP fusion construct.
Indirect immunostaining of PFA fixed mouse 3T3 fibroblasts transfected with a Tom70-GFP fusion construct (green) with Guinea pig anti-GFP (dilution 1 : 500; red) and recombinant rabbit anti-α-Tubulin (cat. no. 302 008, dilution 1 : 500; grey). Nuclei have been visualized by DAPI staining (blue).
Indirect immunostaining of PFA fixed brain section from a VGAT-Cre x AAV-floxed-Chann-YFP mouse (GFP in green) and Guinea pig anti-GFP (dilution 1 : 500; red). Courtesy: Elisa Galliano, PhD, MRC Centre for Developmental Neurobiology, King's College London.
Indirect immunostaining of PFA fixed brain section of a transgenic Cx3CR1-GFP mouse with Guinea pig anti-GFP (cat. no. 132 005, dilution 1 : 400; DAB). Courtesy of Dr. med. Martin Krüger, Institute for Anatomy, University of Leipzig.
Indirect immunostaining of a PFA fixed cortex section from a transgenic CX3CR1-GFP mouse expressing GFP in microglia (green). GFP has been detected with Guinea pig anti-GFP (cat. no. 132 005, dilution 1 : 500, red). Nuclei have been visualized by DAPI staining (blue).
Supplier Page from Synaptic Systems GmbH for GFP Antibody