Fig 2: PYGO2 is the target gene of miR-335-5p. A The level changes of candidate genes were detected by RT-qPCR assay in TNBC cells when miR-335-5p was overexpressed. B PYGO2 expression in TNBC cells compared with that in normal cells was detected by RT-qPCR assay. C SNHG8, miR-335-5p and PYGO2 were significantly enriched in Anti-Ago2 groups, according to the result of RIP assay. D The binding capacity of SNHG8 or PYGO2 with miR-335-5p biotin probes was examined using RNA pull down assay. E, F As illustrated by luciferase reporter assay, the luciferase activities of pmirGLO/SNHG8-WT and pmirGLO/3’UTR-WT were reduced by miR-335-5p overexpression in MDA-MB-231, BT549 or HEK-293 T cells. G The luciferase activities of different reporters in TNBC cells respectively transfected with miR-NC, miR-335-5p mimics and miR-335-5p mimics + SNHG8 were separately detected. *P < 0.05, **P < 0.01

Fig 3: SNHG8 serves as a sponge for miR-335-5p. A, B Subcellular separation assay and FISH assay were conducted to confirm the distribution of SNHG8. C RIP experiment was conducted and it was verified that SNHG8 and miR-335-5p were enriched in the groups of Anti-Ago2. **P < 0.01