Fig 1: Quantitative proteomic analysis of EZH2 targets.a Quantitative mass spectrometry analysis of cells treated with GSK343 (10 µM) for 48 h (n = 3). A heat map presents the relative proportion of downregulated and upregulated proteins. Upregulated proteins have been classified according to different biologic pathways using the PATTERN software. See also supplementary Table 1. b Relative quantification and significance of GAK, AAK1 and AP2M1 expressions obtained by mass spectrometry analysis. c Western blot analysis of the expression of GAK, AAK1, AP2M1 and of its Thr 156 phosphorylated form following GSK343 treatment (n = 4). d Analysis of GAK, AAK1 and AP2M1 mRNA expressions by RT-QPCR (n = 3). e LS174T cells have been stimulated with sn38 for 96 h and emergence was induced after 4 days by serum stimulation. AP2M1 expression was evaluated by western blot analysis at the indicated time (n = 3). f Emergent cells were sorted by flow cytometry after 7 days according to low (PLD) or high (PLS) FSC/SSC values. AP2M1 expression was evaluated by western blot analysis (n = 6), either in the total emergent population (PLC), in the senescent cells (PLS) or in dividing clones (PLD)