Fig 1: Knockdown of XPB mimics the effect of TPL on AR activity in PCa cells. (A) Western blotting analysis of AR phosphorylation and several related proteins in C4-2 cells with XPB depletion. C4-2 cells were infected with lentivirus carrying control shRNA or XPB shRNA. The transduced cells were lysed and subjected to western blotting analysis with the indicated antibodies. (B) Effect of XPB depletion on the AR transactivation activity. 293T or C4-2 cells with stable knockdown of XPB were transiently co-transfected with pGL3.PSA-Luc and pcDNA3.1-AR/WT or -AR/S515E plasmids for 24 h. Luciferase activity was then measured. (C) Effect of XPB depletion on the DNA-binding activity of AR and RNA pol II. C4-2 cells with stable knockdown of XPB were lysed and subjected to ChIP assays with control IgG, anti-AR antibody and anti-RNA pol II S5 antibody. The results are represented as means ±SD of 3 experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001.