Brilliant Violet 421(TM) anti-mouse CD4 100437 from BioLegend

Product Specs
ItemBrilliant Violet 421(TM) anti-mouse CD4
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number100437
CloneGK1.5
NCBI Gene AliasesL3T4, Ly-4
HostRat
ReactivityMouse
Quantity125 µL
ApplicationsFlow Cytometry (FC), Immunocytochemistry (ICC), Immunohistochemistry (IHC)-F
IsotypeIgG2b, Kappa
Conjugate/TagBrilliant Violet 421™
Antibody TypePrimary
NCBI Full Gene NameCD4 antigen
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
ImmunogenMouse CTL clone V4
ClonalityMonoclonal
TargetCD4

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) A photo-triggered self-accelerated nanoplatform for multifunctional image-guided combination cancer immunotherapyNature communicationsAugust 25, 2023Kang X, Zhang Y, Song J, Wang L, Li W, Qi J, Tang BZ.Antibody (1:200, Biolegend, #100306); Brilliant Violet 421™ anti-mouse CD4 Antibody (1:200, Biolegend, #100438); APC anti-mouse CD8a Antibody (1:200, Biolegend, #100712). Characterizations 1H and 13C nuclear magnetic resonance

(2) SMYD3 represses tumor-intrinsic interferon response in HPV-negative squamous cell carcinoma of the head and neckCell reportsJuly 25, 2023Nigam N, Bernard B, Sevilla S, Kim S, Dar MS, Tsai D, Robbins Y, Burkitt K, Sievers C, Allen CT, et al.RRID:AB_492869 Mouse anti-CD3 (145-2C11),Biolegend,Cat# 317317; RRID:AB_1937213 Mouse anti-CD4 (GK1.5),Biolegend,Cat# 100437; RRID:AB_10900241 Mouse anti-CD8 (53–6.7),Biolegend,Cat# 344737 Mouse anti-CD31 (390),Biolegend,Cat#

(3) scRNA-seq profiling of neonatal and adult thymus-derived CD4+ T cells by a T cell origin-time tracing modelJournal of Molecular Cell BiologyApril 20, 2023Yuheng Han, Xinxing Ouyang, Yao Chen, Shujing Lai, Hongxiang Sun, Ningbo Wu, Chun Ruan, Limin Lu, Bing Suantibodies used in this study were as follows: anti-TCRγδ (Thermo Fisher, 12-5711-81), anti-TCRβ (Biolegend, 100438), anti-CD4 (Biolegend, 100438), anti-CD8 (Biolegend, 100752), anti-CD25 (Thermo Fisher Scientific, 17-0251-82),

(4) mRNAs encoding IL-12 and a decoy-resistant variant of IL-18 synergize to engineer T cells for efficacious intratumoral adoptive immunotherapyCell Reports MedicineMarch 17, 2023Irene Olivera, Elixabet Bolaños, Jose Gonzalez-Gomariz, Sandra Hervas-Stubbs, Karina V. Mariño, Carlos Luri-Rey, Iñaki Etxeberria, Assunta Cirella, Josune Egea, Javier Glez-Vaz, et al.use,BioXcell,Clone 3H3; Cat# BE0239; RRID:AB_2687721 Anti-mouse CD4 BV421,BioLegend,Clone GK1-5; Cat# 100438; RRID: AB_11203718 Anti-mouse IL-12/IL-23 p40 PE,BioLegend,Clon C15.6; Cat# 505204; RRID: AB_315368 Anti-mouse

(5) Adenosine deaminase augments SARS-CoV-2 specific cellular and humoral responses in aged mouse models of immunization and challengeFrontiers in immunologyJanuary 1, 2023Gary EN, Tursi NJ, Warner BM, Cuismano G, Connors J, Parzych EM, Griffin BD, Bell MR, Ali AR, Frase D, et al.30 minutes with the following fluorochrome-conjugated anti-mouse antibodies: CD4 (Clone: GK1.5; Cat: 100438), CD8 (Clone: 53-6.7; Cat: 100714), IL-4 (Clone: 11B11; Cat: 504133), IFN-γ (Clone: XMG1.2; Cat: 505810),

(6) An IBD-associated pathobiont synergises with NSAID to promote colitis which is blocked by NLRP3 inflammasome and Caspase-8 inhibitorsGut microbesJanuary 1, 2023Singh R, Rossini V, Stockdale SR, Saiz-Gonzalo G, Hanrahan N, D' Souza T, Clooney A, Draper LA, Hill C, Nally K, et al.anti-mouse CD45 Antibody,Biolegend,Cat# 103128 Brilliant Violet 421™ anti-mouse CD4 Antibody,Biolegend,Cat# 100437 Anti-mouse CD8 PerCP Cy5.5,Biolegend, APC anti-mouse CD69 Antibody,Biolegend,Cat# 104514 Anti-mouse/human

(7) Proximity of immune and tumor cells underlies response to BRAF/MEK-targeted therapies in metastatic melanoma patientsnpj Precision OncologyDecember 1, 2022Chi Yan, Sheau-Chiann Chen, Gregory D. Ayers, Caroline A. Nebhan, Joseph T. Roland, Vivian L. Weiss, Douglas B. Johnson, Ann Richmond(BioLegend, #103116, 1:250), CD3-PerCP/eFluor 710 (eBioscience, #460032-80, 1:200), CD4-BV421 (BioLegend, #100438, 1:200), CD8-AlexaFlour700 (BioLegend, #100729, 1:500), and CD69-APC (BioLegend, #104513, 1:100). After

(8) Enhancement of antitumor immunotherapy using mitochondria-targeted cancer cell membrane-biomimetic MOF-mediated sonodynamic therapy and checkpoint blockade immunotherapyJournal of NanobiotechnologyDecember 1, 2022Jiali Luo, Xue Wang, Zhan Shi, Yiqing Zeng, Liangcan He, Jing Cao, Yu Sun, Tao Zhang, Pintong HuangBiolegend, UK), PE/Cyanine7 anti-mouse CD3 (100220, Biolegend, UK), Brilliant Violet 421™ anti-mouse CD4 (100438, Biolegend, UK), Alexa Fluor® 647 anti-mouse Foxp3 (126408, Biolegend, UK). Cells collected from DLNs

(9) A Novel Selective Axl/Mer/CSF1R Kinase Inhibitor as a Cancer Immunotherapeutic Agent Targeting Both Immune and Tumor Cells in the Tumor MicroenvironmentCancers (Basel)October 2, 2022Yeejin Jeon, Hwankyu Kang, Yeongin Yang, Dongsik Park, Baejung Choi, Jeongjun Kim, Jaeseung Kim, Kiyean Nam(BioLegend; #103149, clone 30-F11), CD3-BUV395 (BD Biosciences; #740268, clone 17A2), CD4-BV421 (BioLegend; #100438, clone GK1.5), CD8-FITC (MBL International Corporation, Woburn, MA, USA; #D271-4, clone KT15), and SIINFEKL-H-2Kb

(10) Evaluation of Glutaminolysis in T CellsCurr ProtocSeptember 1, 2022Tajima M, Strober W.(Thermo Fisher, cat. # 00–5523-00) Brilliant Violet 421™ anti-mouse CD4 Antibody (Biolegend, cat # 100437) FITC anti-mouse IL-17A Antibody (Biolegend, cat # 506907) PE anti-mouse IL-4 Antibody (Biolegend,

(11) B7H3-dependent myeloid-derived suppressor cell recruitment and activation in pulmonary fibrosisFrontiers in ImmunologyAugust 15, 2022Tianju Liu, Francina Gonzalez De Los Santos, Andrew E. Rinke, Chuling Fang, Kevin R. Flaherty, Sem H. PhanrmIL2 (R&D Systems) for 3 days. The cells were collected and stained with CD4-BV421 (BioLegend, Cat# 100437, clone GK1.5) and CD8-APC (BioLegend, Cat# 100711, clone 53-6.7). The intensity of incorporated CFSE

(12) Nanoliposome C6‐Ceramide in combination with anti‐CTLA4 antibody improves anti‐tumor immunity in hepatocellular cancerThe FASEB JournalJuly 15, 2022Xiaoqiang Qi et al.manufacturer's instruction. Fluorochrome‐labeled Abs for CD3 (CAT#100236), CD8 (CAT#100748), CD4 (CAT#100438), CD45 (CAT#147708), CD69 (CAT#104530), CD25 (CAT#101908), NK 1.1 (CAT#156507) and CD49b (CAT#103515)

(13) Inhibition of myeloid-derived suppressor cell arginase-1 production enhances T-cell-based immunotherapy against Cryptococcus neoformans infectionNature communicationsJuly 14, 2022Li YN, Wang ZW, Li F, Zhou LH, Jiang YS, Yu Y, Ma HH, Zhu LP, Qu JM, Jia XM.APC-anti-mouse CD8 (Clone 53-6.7, Biolegend, 100712, 1:100); BV421-anti-mouse CD4 (Clone GK1.5, Biolegend, 100438, 1:100); APC-anti-mouse IFNγ (Clone XMG1.2, Biolegend, 505810, 1:100); PE-anti-mouse IL-17A (Clone ebio17B7,

(14) Poroptosis: A form of cell death depending on plasma membrane nanopores formationiScienceJune 17, 2022Hao Li, Zihao Wang, Xiaocui Fang, Wenfeng Zeng, Yanlian Yang, Lingtao Jin, Xiuli Wei, Yan Qin, Chen Wang, Wei LiangCD11c APC,Biolegend,Cat # 117310; RRID:AB_313779 Anti-mouse CD4 Brilliant Violet 421™,Biolegend,Cat # 100437; RRID: AB_10900241 Anti-mouse CD45 Brilliant Violet 605™,Biolegend,Cat # 103140; RRID: AB_2562342 Anti-mouse

(15) A DNA vaccine candidate delivered by an electroacupuncture machine provides protective immunity against SARS-CoV-2 infectionNPJ vaccinesJune 3, 2022Tzeng TT, Chai KM, Shen KY, Yu CY, Yang SJ, Huang WC, Liao HC, Chiu FF, Dou HY, Liao CL, et al.the surface stains CD3 (1:100; Biolegend, cat# 100320, clone 145-2C11), CD4 (1:100, Biolegend, cat# 100437, clone GK1.5), CD8 (1:100, Biolegend, cat# 100752, clone 53-6.7), CD44 (Biolegend, cat# 103028, clone

(16) Chitooligosaccharides Improve the Efficacy of Checkpoint Inhibitors in a Mouse Model of Lung CancerPharmaceuticsMay 12, 2022Johansen AZ, Carretta M, Thorseth ML, Khan S, Fjæstad KY, Brøchner CB, Linder H, Ankjærgaard C, Donia M, Chen I, et al.103107, BioLegend, San Diego, CA, USA), PDGFRα-BV605 (cat. No. 135916, BioLegend), CD4-BV421 (cat. No. 100438, BioLegend), CD8-PerCP-Cy5.5 (cat. No. 551162, BD Pharmingen, San Jose, CA, USA), CD11b-PE/Cy7 (cat.

(17) Germinal center expansion but not plasmablast differentiation is proportional to peptide-MHCII density via CD40-CD40L signaling strengthCell reportsMay 3, 2022Jing Z, McCarron MJ, Dustin ML, Fooksman DR.RA3-6B2),BioLegend,Cat# 103224; RRID:AB_313007 Rat monoclonal anti-CD4 BV421 (clone GK1.5),BioLegend,Cat# 100438; RRID:AB_11203718 Rat monoclonal anti-CD16/CD32 (clone 2.4G2),Bio X Cell,Cat# CUS-HB-197; RRID:AB_2687830 Rat

(18) Negative Immune Checkpoint Protein, VISTA, Regulates the CD4+ Treg Population During Sepsis Progression to Promote Acute Sepsis Recovery and SurvivalFrontiers in ImmunologyMarch 24, 2022Chyna C. Gray, Bethany Biron-Girard, Michelle E. Wakeley, Chun-Shiang Chung, Yaping Chen, Yael Quiles-Ramirez, Jessica D. Tolbert, Alfred Ayalawith the following monoclonal anti-mouse antibodies: CD4-BV421 (BioLegend, San Diego, CA, USA, Cat# 100438, RRID : AB_11203718), CD8a-BV510 (BioLegend Cat# 100752, RRID : AB_2563057), CD69-FITC (Miltenyi Biotec

(19) Maintained partial protection against Streptococcus pneumoniae despite B‐cell depletion in mice vaccinated with a pneumococcal glycoconjugate vaccineClinical & Translational ImmunologyJanuary 1, 2022Giuseppe Ercoli, Elisa Ramos‐Sevillano, Emma Pearce, Sara Ragab, David Goldblatt, Gisbert Weckbecker, Jeremy S Brownstained with the following antibodies: CD19 (BioLegend, 152409), CD3 (BioLegend, 362703), CD4 (BioLegend, 100438), CD8 (BioLegend, 155004), CD11b (BioLegend, 101245), CD11c (BioLegend, 117317), and Ly‐6G (BioLegend,

(20) Engineering Ligand and Receptor Pairs with LIPSTIC to Track Cellâ€Cell InteractionsCurrent ProtocolsDecember 6, 2021Dafne Alberti, Michelle Guarniero, Agnieszka K. Maciola, Enrico Dotta, Giulia PasqualAnti-biotin-APC (e.g., Miltenyi Biotec, cat. no. 130-113-288)Anti-CD4-BV421 (e.g., Biolegend, cat. no. 100437)Anti-CD19-PE-Cy7 (e.g., Biolegend, cat. no. 115520) 96-well U-bottom plates37°C, 5% CO2 incubator for

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(1)

4/21/2020Detection of CD4 T Cells in Mouse Tissues Preeyam PatelCompatible with the use of many different antibodies in a panel. Clean and great detection.
(1)

2/16/2016Good Marker for Sorting Naive CD4 T Cells Adam ColemanWe study T cell responses to antigen presenting cells. This is an excellent antibody for gating populations of CD4+ T cells in murine spleens.

Brilliant Violet 421(TM) anti-mouse CD4 from BioLegend

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Brilliant Violet 421(TM) anti-mouse CD4 from BioLegend

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