Fig 2: Protein expression of pluripotency markers in uterine cells isolated from control cows and from cows with adenomyosis. NANOG a OCT4 b and SOX2 c protein expression in uterine stromal cells. NANOG d OCT4 e and SOX2 f protein expression in uterine myometrial cells. Data were normalized against glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Bars represent the mean ± SEM. Asterisks indicate statistical differences between uterine normal and adenomyotic tissue (*P < 0.05; **P < 0.01), as determined by Student’s t-test. Representative blots for NANOG, OCT4, SOX2 and GAPDH are shown below the graphs g. MM – molecular weight marker, C – cells obtained from control cows, ADENO – cells obtained from cows with adenomyosis

Fig 3: Protein expression of NANOG a, OCT4 b and SOX2 c in bovine uterine tissues obtained from control cows and from cows with adenomyosis. Data were normalized against glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Bars represent the mean ± SEM. There were no statistical differences between uterine normal and adenomyotic tissues (P > 0.05), as determined by Student’s t-test. Representative blots for NANOG, OCT4, SOX2 and GAPDH are shown below the graphs d. MM – molecular weight marker, C – tissues obtained from control cows, ADENO – tissues obtained from cows with adenomyosis

Fig 4: mRNA expression of pluripotency markers NANOG a, OCT4 b and SOX2 c in uterine tissues obtained from control cows and from cows with adenomyosis. Data were normalized against glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Bars represent the mean ± SEM. Asterisks indicate statistical difference between uterine normal and adenomyotic tissues (P < 0.05), as determined by Student’s t-test. C – tissues obtained from control cows, ADENO – tissues obtained from cows with adenomyosis

Fig 5: mRNA expression of pluripotency markers in uterine cells isolated from control cows and from cows with adenomyosis. NANOG a OCT4 b and SOX2 c mRNA expression in uterine stromal cells. NANOG d OCT4 e and SOX2 f mRNA expression in uterine myometrial cells. Data were normalized against glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Bars represent the mean ± SEM. Asterisks indicate statistical differences between uterine normal and adenomyotic tissue (*P < 0.05; **P < 0.01), as determined by Student’s t-test. C – cells obtained from control cows, ADENO – cells obtained from cows with adenomyosis