Anti-3-Nitrotyrosine antibody [7A12AF6] ab110282 from Abcam

Product Specs
ItemAnti-3-Nitrotyrosine antibody [7A12AF6]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab110282
IsotypeIgG2b
Clone7A12AF6
ClonalityMonoclonal
Quantity100 UG
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, In-Cell ELISA, Western blot
Conjugate/TagUnconjugated
HostMouse, Nude mouse
Antibody TypePrimary
FormatThe antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation
Concentration1 mg/ml
ReactivitySpecies independent
Target3-Nitrotyrosine

Add to Compare List

Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) The NO Answer for Autism Spectrum DisorderAdvanced ScienceMay 22, 2023Manish Kumar Tripathi, Shashank Kumar Ojha, Maryam Kartawy, Wajeha Hamoudi, Ashwani Choudhary, Shani Stern, Adi Aran, Haitham Amalcocktail (#5872) were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti‐3Ntyr antibody (ab110282), Anti‐homer 1 (ab184955), Anti‐NMDAR1 antibody (ab109182), Anti‐SLC32A1/VGAT antibody (1b235952), and

(2) Human sperm proteome reveals the effect of environmental borne seminal polyaromatic hydrocarbons exposome in etiology of idiopathic male factor infertilityFrontiers in Cell and Developmental BiologyMay 16, 2023Jasmine Nayak, Soumya Ranjan Jena, Sugandh Kumar, Sujata Kar, Anshuman Dixit, Luna SamantaHSP90β (sc-59578, Santacruz, Mouse), anti-4-HNE (ab46545, Rabbit, Abcam) and anti-3-nitro-tyrosine (ab110282, Mouse, Abcam) For maintaining biological and technical variability, triplicate tests were performed

(3) Apoptotic vesicles resist oxidative damage in noise-induced hearing loss through activation of FOXO3a-SOD2 pathwayStem cell research & therapyApril 15, 2023Huang X, Kou X, Zhan T, Wei G, He F, Mao X, Yang H.incubated with anti-4-hydroxynonenal (4-HNE, Abcam, #ab46545, 1:200), anti-3-nitrotyrosine (3-NT, Abcam, #ab110282, 1:400) overnight at 4 °C and secondary antibody (CST, #8889, 1:400) for 1 h at room temperature. Then

(4) AIF Overexpression Aggravates Oxidative Stress in Neonatal Male Mice After Hypoxia–Ischemia InjuryMolecular NeurobiologyNovember 1, 2022Tao Li et al.donkey serum in PBS for 30 min, the monoclonal mouse anti-3-NT (1.5:1000 dilution, 7A12AF6, Abcam, ab110282), polyclonal rabbit anti-DCX (1:500 dilution, Abcam, ab18723), or monoclonal mouse anti-BrdU (1:200

(5) Mitochondrial-Targeted Therapies Require Mitophagy to Prevent Oxidative Stress Induced by SOD2 Inactivation in Hypertrophied CardiomyocytesAntioxidants (Basel, Switzerland)April 6, 2022Peugnet V, Chwastyniak M, Mulder P, Lancel S, Bultot L, Fourny N, Renguet E, Bugger H, Beseme O, Loyens A, et al.software (Zen 2.3) (Zeiss). 2.7. Antibodies The primary antibodies used were 3-nitrotyrosine (mouse, ab110282, Abcam, Cambridge, UK, 1/1000 WB); aconitase 2 (rabbit, GTX109736, CliniSciences, Nanterre, Paris, France,

(6) Treatment with the Ferroptosis Inhibitor Ferrostatin-1 Attenuates Noise-Induced Hearing Loss by Suppressing Ferroptosis and ApoptosisOxidative medicine and cellular longevityJanuary 1, 2022Ma PW, Wang WL, Chen JW, Yuan H, Lu PH, Gao W, Ding XR, Lun YQ, Liang R, He ZH, et al.antibodies at 4°C overnight: 4-HNE antibody (GeneTex, GTX17571; 1 : 100) and 3-NT antibody (Abcam, ab110282; 1 : 100). After 3 washes with PBS, the specimens were stained with Alexa Fluor 594-conjugated donkey

(7) Decreased proteasomal cleavage at nitrotyrosine sites in proteins and peptidesRedox BiologyOctober 1, 2021Christiane Ott, Florencia Tomasina, Nicolás Campolo, Silvina Bartesaghi, Mauricio Mastrogiovanni, Alejandro Leyva, Carlos Batthyány, Walter Meinl, Tilman Grune, Rafael Radifollowing antibodies were used: anti-Cyt c antibody (#136F3, Cell Signaling) and anti-NO2Y antibody (ab110282, Abcam). Fluorescent-conjugated secondary antibodies (Li-COR Biosciences) were used according to the

(8) Deficiency of Klc2 Induces Low-Frequency Sensorineural Hearing Loss in C57BL/6 J Mice and HumanMolecular neurobiologySeptember 1, 2021Fu X, An Y, Wang H, Li P, Lin J, Yuan J, Yue R, Jin Y, Gao J, Chai R.mab1622), anti-caspase3 (CST, 9661S), anti-Lc3b(CST,2775S), anti-prestin (sc-22692), anti-3NT (Abcam, ab110282), anti-4HNE (Abcam, ab46545), anti-Kcnma1 (Alomone, APC-021), anti-HAtag (CST, 3724S). AAV preparation

(9) Central Acting Hsp10 Regulates Mitochondrial Function, Fatty Acid Metabolism, and Insulin Sensitivity in the HypothalamusAntioxidants (Basel)April 30, 2021Kristina Wardelmann et al.Total OXPHOS Rodent WB Antibody Cocktail (Abcam #ab110413, 1:2000), GFAP (Abcam, Cambridge, UK #7260), 3-Nitrotyrosine (Abcam #ab110282, 1:1000), β Actin+HRP (Sigma-Aldrich A3854, 1:10,000). Membranes were incubated with peroxidase-conjugated

(10) Ebselen reduces cigarette smoke‐induced endothelial dysfunction in miceBritish Journal of PharmacologyApril 1, 2021Kurt Brassington, Stanley M. H. Chan, Huei Jiunn Seow, Aleksandar Dobric, Steven Bozinovski, Stavros Selemidis, Ross Vlahosnitroprusside (Thermo Fisher, USA); eNOS/NOS3 RRID: AB_2533121 (Thermo Fisher Scientific, USA), 3‐NT RRID: AB110282 (Abcam, USA); Goat anti‐mouse IgG (H + L) secondary antibody, Alexa Flour Plus 488 RRID: AB_2633275

(11) Effect of TTLL12 on tubulin tyrosine nitration as a novel target for screening anticancer drugs in vitroOncology LettersOctober 8, 2020Lingli Xue, Yan Zeng, Chuan Fang, Wei Cheng, Yadong LiAbcam), tributyl phosphate (TBP) (1:800; cat. no. ab220788; Abcam) and nitrotyrosine (1:1,000; cat. no. ab110282; Abcam) at a temperature of 37°C for 3 h. Following the primary incubation, membranes were incubated

(12) TGF-β1 attenuates mitochondrial bioenergetics in pulmonary arterial endothelial cells via the disruption of carnitine homeostasisRedox biologySeptember 1, 2020Sun X, Lu Q, Yegambaram M, Kumar S, Qu N, Srivastava A, Wang T, Fineman JR, Black SM.anti-nitrotyrosine antibody was obtained from EMD Millipore (Billerica, MA). The anti-3-Nitrotyrosine antibody (cat#:ab110282) was obtained from Abcam (Cambridge, MA). The anti-CrAT was obtained from Proteintech (Rosemont, IL).

(13) Age-Related Maintenance of the Autophagy-Lysosomal System Is Dependent on Skeletal Muscle TypeOxidative Medicine and Cellular LongevityJuly 24, 2020Raquel Fernando, José Pedro Castro, Tanina Flore, Stefanie Deubel, Tilman Grune, Christiane OttK63-linkage specific polyubiquitin (Cell Signaling no. 5621), rabbit polyclonal 3-nitrotyrosine (Abcam, ab110282), mouse monoclonal pentosidine (BioLogo, PEN012) and rabbit monoclonal LAMP1 (Cell Signaling no. 3243).

(14) Genetically modified macrophages accomplish targeted gene delivery to the inflamed brain in transgenic Parkin Q311X(A) mice: importance of administration routesScientific reportsJuly 16, 2020Haney MJ, Zhao Y, Fay J, Duhyeong H, Wang M, Wang H, Li Z, Lee YZ, Karuppan MK, El-Hage N, et al.separation. Immunoblots were labeled with primary antibodies against 3-Nitrotyrosine (NT)—1:200 (Abcam, ab110282), PSD-95—1:200 (Abcam, ab76115), NMDAR2B—1:200 (Abcam, ab65783), NF-kB—1:200 (Santa Cruz Biotechnology,

(15) Aging affects sex- and organ-specific trace element profiles in miceAging (Albany NY)July 3, 2020Kristina Lossow et al.blocking solution with 0.1 % Tween 20 (Merck) and incubated overnight at 4°C. Mouse anti-3-NT (Abcam, ab110282, 1:1,000), rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH; Abcam, ab37168, 1:10,000), mouse

(16) Exogenous PP2A inhibitor exacerbates the progression of nonalcoholic fatty liver disease via NOX2-dependent activation of miR21American Journal of Physiology-Gastrointestinal and Liver PhysiologyOctober 1, 2019Muayad Albadrani et al.The primary antibody for CD68 (1:300, ab125212, Abcam), ␣-SMA (1:250, ab32575, Abcam), 3NT (1:250, ab110282, Abcam), P-65 (1:300, SC-8008, Santa Cruz Biotechnology), and p-AKT (1:300, SC-293125, Santa Cruz Biotechnology)

(17) A Delphinidin-Enriched Maqui Berry Extract Improves Bone Metabolism and Protects against Bone Loss in Osteopenic Mouse ModelsAntioxidants (Basel, Switzerland)September 10, 2019Nagaoka M, Maeda T, Chatani M, Handa K, Yamakawa T, Kiyohara S, Negishi-Koga T, Kato Y, Takami M, Niida S, et al.anti-I-κB antibody (E130, ab32518, Abcam, Cambridge, UK), and mouse anti-nitrotyrosine antibody (7A12AF6, ab110282; recognize only protein-bound nitrotyrosine, Abcam) followed by appropriate Alexa Fluor 488® or 594®-labeled

(18) Low-protein diet supplemented with ketoacids delays the progression of diabetic nephropathy by inhibiting oxidative stress in the KKAy mice modelBritish Journal of NutritionJanuary 14, 2018Dongmei Liu, Ming Wu, Lin Li, Xiang Gao, Bo Yang, Shuqin Mei, Lili Fu, Changlin Meipolyvinylidene diﬂuoride membrane, and then incubated with the primary antibody against nitrotyrosin (NT) (ab110282; Abcam)(40). The enhancedchemiluminescence system was used to detect the signals. The concentration

(19) Age-related oxidative changes in pancreatic islets are predominantly located in the vascular systemRedox BiologyDecember 29, 2017Richard Kehm, Jeannette König, Kerstin Nowotny, Tobias Jung, Stephanie Deubel, Sabrina Gohlke, Tim Julius Schulz, Annika Höhnanti-iNOS antibody (ab178945, Abcam, Cambridge, United Kingdom) and mouse anti-3-Nitrotyrosine antibody (ab110282, Abcam, Cambridge, United Kingdom) were used as primary antibodies for IF staining of PDX-1, p16Ink4a,

(20) Dietary Vitamin D3 Restriction Exacerbates Disease Pathophysiology in the Spinal Cord of the G93A Mouse Model of Amyotrophic Lateral SclerosisPloS oneJanuary 1, 2015Moghimi E, Solomon JA, Gianforcaro A, Hamadeh MJ.3-NY, GPx1, Bax, Bcl-2, pro-caspase 3) against 4-HNE (1:800; Abcam, ab46545), 3-NY (1:1000; Abcam, ab110282), SOD2 (1:8000; Abcam, ab13533), catalase PLOS ONE | DOI:10.1371/journal.pone.0126355 May 28, 2015 4

Be the First to Write a Review!

Anti-3-Nitrotyrosine antibody [7A12AF6] from Abcam

Abcam

Citations (24)

Reviews

Anti-3-Nitrotyrosine antibody [7A12AF6] from Abcam

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map