Fig 1: Cardiac marker expression on hESC-MPs and 5-AZA or TGF-ß1 treated cells.A. Phase contrast microscopy of hESC-NKX2.5 cell line submitted to our MP derivation protocol demonstrating GFP expression reporting NKX2.5 in our MPs. 5-AZA and TGF-ß1 treatments do not modify the GFP expression (last two panels). B. Overlay representing GFP expression (reporting NKX2.5) at day 30 of treatment by 5-AZA or TGF-ß1. C. Overlay representing flow cytometry analysis of cardiac myosin heavy chain (CMH), Cardiac Troponin T (CTNT), Homeobox protein NKX2.5, The sodium-calcium exchanger (NCX1), alpha smooth muscle actin (a-SMA), Cardiac Troponin I (CTNI) and Myosin regulatory light chain (MRCL3) in hES-MPs untreated or treated for 30 days by 5-AZA or TGF-ß1. D. Confocal microscopy imaging of the expression of cardiac marker genes. MHC, MRCL3, aSMA demonstrate a striated pattern. CTNI demonstrated a weak striated pattern. NKX2.5 is clearly labeled in the nucleus. (x 600).